Figure 1
From: Biocomputational prediction of non-coding RNAs in model cyanobacteria
Pipeline for comparative prediction of RNA elements and ncRNAs. Intergenic sequences of at least 50 nt were gathered from four cyanobacterial genomes and locally aligned using BLASTN. Sequences which directly produced a significant blast hit (E-value < 10-5) or which were connected by a chain of such hits were gathered into clusters ("single-linkage clustering"). After an additional unification step of overlapping sequences within each cluster the resulting clusters and their complement clusters were scored using RNAz [26]. The inset shows how this information is provided in the internet, together with the location within the compared genomes, using the top-scoring cluster (CLID 80) as an example.