Figure 1

Flow diagram of the cell cycle signature (CCS) method. (A) CCS_cycling consists of genes which preferentially express in cycling cells and in a cell cycle-regulated manner, representing the entire cell cycle. Each CCS subset consists of genes whose expressions peak at specific stages of the cell cycle, representing the corresponding stages. (B) From the given total gene dataset, the cycling gene dataset is created by extracting the expression values of CCS_cycling genes. Both datasets are independently quantile normalized and the CCS scores are calculated for each.