Genomic organization and classification of the bovine WC1 genes and expression by peripheral blood gamma delta T cells
© Herzig and Baldwin; licensee BioMed Central Ltd. 2009
Received: 17 December 2008
Accepted: 24 April 2009
Published: 24 April 2009
WC1 co-receptors are group B scavenger receptor cysteine-rich molecules that are found exclusively on γδT cells and are thought to be encoded by a multi-gene family. Previous studies have shown γδT cells that respond to a particular stimulus have unique WC1 molecules expressed. Prior to the onset of the studies described here only one full-length WC1 nucleotide sequence was publicly available, though three WC1 molecules had been distinguished based on monoclonal antibody reactivity. Furthermore, the number of WC1 genes found in the bovine genome and their sequences had not yet been resolved.
By annotating the bovine genome Btau_3.1 assembly, here we show the existence of 13 members in the WC1 gene family and their organization within two loci on chromosome 5 including three distinct exon-intron gene structures one of which coded for a potentially more primitive and smaller WC1 molecule that is similar to the swine WC1 gene. We also provide cDNA evidence as verification for many of the annotated sequences and show transcripts for isoforms derived by alternative splicing.
It is possible that WC1 diversity contributes to functional differences that have been observed between γδT cell populations. The studies described here demonstrate that WC1 molecules are encoded by a large, multi-gene family whose transcripts undergo extensive alternative splicing. Similar to other non-rearranging immunoreceptors, it is likely that the WC1 gene repertoire underwent expansion in order to keep pace with rapidly changing ligands.
γδT cells play important roles in immune responses by their capacity to produce IFN-γ [1, 2], down-modulate immune responses following pathogen clearance [3, 4] and develop recall responses to antigen [1, 5–7]. Thus it is important to understand the manner by which they are engaged in immune responses. Workshop cluster 1 (WC1) co-receptors are group B scavenger receptor cysteine-rich (SRCR) transmembrane glycoproteins that are unique to γδT cells [8–10] of some species including cattle , sheep (T19) [9, 12] and swine , but have not yet been identified for humans or mice. Since different molecular forms of WC1 are found on functionally distinct subpopulations of bovine γδT cells [2, 14], we propose it to be a pattern recognition molecule and/or involved in regulating signaling through the T cell receptor (TCR). Support for this comes from other group B SRCR molecules found on immune system cells including CD5 which is a positive and negative regulator of TCR and B cell receptor signaling  and CD6 which functions as an adhesion molecule  and for activation [17–19]. Also CD163, predominantly expressed on monocytes and macrophages , plays an important role in down-regulating inflammatory responses  while DMBT1 on macrophages functions as a pattern recognition molecule (for review see ).
Diversity among the immune system-associated SRCR molecules can result from alternative splicing of their individual genes. CD5 and CD6 undergo alternative splicing within the cytoplasmic tail region [23–25] but function of these isoforms is unclear. Both CD163 and DMBT1 undergo extensive alternative splicing of the extracellular and cytoplasmic coding regions, and a CD163 isoform lacking the transmembrane region has been described [22, 26, 27]. While the predominant short-tailed CD163 variant mediates ligand internalization and degradation, the function of the long-tail CD163 variant is unknown  as is the role of DMBT1 splice variants. Alternatively spliced variants have also been reported for swine and sheep WC1 orthologs, splicing occurs within both extracellular and cytoplasmic domains, and isoforms lacking the transmembrane region have been identified [29, 30]. Thus while isoform formation appears to be common among SRCR molecules, diversity of immunoreceptors can also result from multiple genes and in this regard WC1 is distinguished from the others.
Although WC1 is unique among group B SRCR molecules because it alone is coded for by a large multi-gene family [23, 24, 26, 31, 32], other non-SRCR immunoreceptors are coded for by multi-gene families including the C-type lectin-like Ly49. Ly49 is a major receptor that regulates natural killer (NK) cells and some T cells. It has 15 functional genes in mice [33, 34], but only a single copy in humans [35, 36] and cattle . Additionally, the killer Ig-like receptor (KIR) multi-gene family found on NK and T cells of primates [38, 39] and cattle  encodes products that are functionally similar to but structurally distinct from those of the Ly49 family. Both the KIR and Ly49 gene families are believed to have resulted from rapid repeated gene duplication [33, 40, 41]. It has been speculated that these families of closely related non-rearranging immunoreceptors have evolved to include large numbers of duplicated genes to keep pace with rapidly changing ligands. This appears to be the case for MHC class I molecules, which comprise a large family and are the ligands for Ly49 receptors in mice and rats and for KIR family members in cattle and primates [33, 37, 42, 43]. We hypothesize that in the case of γδT cells WC1 is similarly important in inducing and perpetuating γδT cell responses thus necessitating the expansion of the WC1 repertoire. This is supported by previous studies showing that antigen responsive WC1+ γδT cells express a restricted set of TCR gamma genes regardless of the WC1 molecule found on the cells  and that subpopulations of γδT cells that respond to different stimuli express different WC1 molecules [2, 14].
While three distinct WC1 molecular forms have been distinguished based on partial sequence and their reactivity with monoclonal antibodies (mAb) , the number of WC1 genes found in the bovine genome and their sequences has yet to be resolved. Only one full-length nucleotide sequence is publicly available (i.e. WC1.1, known as the archetypal WC1) . For sheep WC1 (also known as T19), over 50 genes are predicted [46, 47] based on Southern blot analysis while for cattle many related genes are predicted based on Southern blots  and 13 based on cDNA sequences of the WC1 intracytoplasmic tails . While the number of genes coding for porcine WC1 proteins is unknown, swine have a more abbreviated version of WC1 which has 6 SRCR domains. This suggests swine WC1 genes did not undergo the same internal SRCR domain-duplication that occurred in cattle  since previously described bovine WC1 molecules have 11 SRCR domains . By annotating the bovine genome Btau_3.1 assembly, here we demonstrate the existence of 13 members in the WC1 family and show their organization within two loci including a bovine gene coding for the putatively more primitive and smaller swine-like WC1 molecule. We also provide cDNA evidence as verification for many of the annotated sequences as well as for isoforms derived by alternative splicing.
Annotation of WC1 genes
Chromosomal location of WC1 genes
Deduced amino acid sequences of the 13 annotated WC1 genes described here were aligned and shown compared with the archetypal WC1 sequence (Figures 3, 4, 5 and 6). Genes are grouped according to their distinct gene structure (i.e. Types I – III) and it is evident that genes of a particular type have sequence similarity. However, despite WC1-4 and WC1-9 having different types of gene structures their extracellular regions are very similar although differences within their intracytoplasmic tail regions occur as would be expected since WC1-9 has an extra exon. When WC1 gene distribution on chromosome 5 was evaluated it was found that their relatedness based on type did not correlate entirely with their genomic location (refer to Figure 2).
cDNA evidence for WC1 distinct gene structures
mRNA derived from bovine PBMC was analyzed to confirm the transcription of the multiple genes annotated above. To verify that cDNA sequences (designated with a prefix of 'CH') obtained were indeed representative of multiple genes, instead of genetic variation among animals, all reactions were performed using mRNA from a single animal. Also, because WC1+ γδT cells with different WC1 molecular forms respond to different activation stimuli [2, 14], reactions were performed with pooled cDNA from ConA-activated, leptospira-activated and ex vivo PBMC in order to maximize the number of WC1 transcripts amplified.
Primers used for WC1 transcript amplification and for cDNA clone sequencing reactions
Primers used for gene amplification:
Primers used for sequencing reactions:
cDNA evidence for transcription of all three WC1 distinct gene structure typesa
Domain 1 is the most variable SRCR domain
Deduced amino acid sequence percent identity within individual, non-redundant WC1 domains
Number of sequences compareda
Highest percent identity
Lowest percent identity
WC1 cDNA sequences and classification based on annotated genomic sequences
cDNA clone name
GenBank accession number
Exon-intron structure type
Domain 1 comparisons also provided evidence for transcription of two more genes. CH590 and CH601 Domain 1 sequences (Wang F, Herzig CTA, Baldwin CL, Telfer JC: Response of bovine γδT cells to Leptospira requires WC1 expression. Genes and Immunity, submitted) corresponded to those of the genes designated WC1-5 and WC1-10, respectively, for which corresponding full-length transcripts were not found above. Since the relationship between other cDNA and genomic sequences seen in Table 3 were largely maintained when just Domain 1 sequences were compared here (Figure 7), this is reasonable evidence for transcription of WC1-5 and WC1-10. Finally, one Domain 1 cDNA sequence, CH583, lacked genomic corresponding sequence and thus was named WC1-nd2 and suggests a fifteenth WC1 gene.
Intracytoplasmic region analysis and classification
It could be reasoned that the most divergent of the 11 WC1 SRCR domains, i.e. Domain 1 as shown above, would be the ligand binding region of the WC1 co-receptor and thus that the WC1 Domain 1 sequence associated with a particular γδT cell could influence its response to various stimuli. However, it is also possible that variation in the intracytoplasmic tail regions of WC1 could convey signaling differences which would influence the outcome of receptor ligation. This is supported by previous observations regarding the tail function . Therefore, comparisons of WC1 intracytoplasmic region cDNA sequences with genomic intracytoplasmic sequences were done.
Evidence for isoform generation
There was no apparent pattern to WC1 splicing. It was most common for entire SRCR domains to be missing, though in one case (CH485) half of a domain was missing but it still appeared to result in a viable transcript. Most often five contiguous SRCR domains were missing so that the resulting transcript contained six SRCR domains; however, transcripts lacking anywhere from one to six full SRCR domains were identified. WC1 transcripts lacking the transmembrane regions (thus are putatively secreted molecules) were also identified as otherwise full-length WC1 sequences as well as for WC1 splice variants lacking either four or five SRCR domains. While two WC1 cDNA sequences (CH533 and CH520) have shorter intracytoplasmic domains due to premature stop codons, there was no evidence of alternative splicing within the intracytoplasmic domain.
To better characterize the bovine WC1 co-receptor family we annotated the WC1 genes in the bovine genome Btau_3.1 assembly and identified 13 WC1 genes distributed between two loci on chromosome 5. This included a novel WC1 gene that more closely resembles swine WC1 than it does previously identified ruminant WC1 genes. Work is ongoing to resolve the gaps in the genome assembly of chromosome 5. However, the number of WC1 genes in the multi-gene family agrees reasonably well with previous reports estimating the occurrence of many related WC1 genes based on Southern blots  and of 13 WC1 genes based on cDNA analysis of intracytoplasmic tail region transcripts  but is fewer than the 50 WC1 genes predicted for sheep by Southern blotting [46, 47]. The distribution of WC1 genes between two loci is reminiscent of the two TCR gamma loci for cattle and sheep (TRG1 and TRG2) where it is predicted that a number of duplication events and a subsequent translocation event resulted in the formation of the TRG2 locus . It is possible that similar events contributed to the formation of the WC1 loci because the distribution of WC1 genes based on sequence similarity does not support the idea that a single WC1 locus underwent duplication. Also, interestingly, the distribution of the three distinct exon-intron structures identified here for the WC1 genes (i.e. Type I, II or III) among the two loci also does not support locus duplication alone because the Type II and III genes are found only within a single locus.
We verified the gene annotations by amplifying and sequencing WC1 cDNA from bovine cells derived from a single animal. cDNA evidence confirmed the presence of at least a single functional gene, in an individual animal, for each of the three types of genes differentiated by their exon-intron structure. It is important to note that the profile of WC1 transcripts obtained from this individual animal is not necessarily representative of all WC1 genes expressed. This is despite using an experimental design in which cDNA from several conditions was pooled prior to PCR as well as using a variety of primers in an attempt to avoid biased amplification of particular WC1 transcripts. With regard to this, it was notable that transcripts representative of archetypal WC1.1, the only full-length sequence previously published , were not found to be abundant, to the extent that it was necessary to design a separate primer to preferentially amplify archetypal WC1.1 transcripts. Nevertheless, we confirmed the presence of 8 out of 13 WC1 genes based on cDNA sequences that corresponded to genomic sequences while cDNA evidence for WC1-2, WC1-6, WC1-7, WC1-8 and WC1-12 was not found. (Because many of those gene sequences are partial, it cannot be ruled out that cDNA evidence does exist for those genes but could not be classified as such at this point). Although all cDNA transcript sequences varied to some extent from corresponding genomic sequences, variations found between cDNA and related genomic sequences are most likely attributable to variation between animals due to single nucleotide polymorphisms. Indeed, even within a single animal there was preliminary evidence of allelic polymorphism (C.T.A. Herzig, unpublished data). Only two WC1 sequences derived from RT-PCR, CH525 and CH583, lacked any corresponding genomic sequence and were assigned the gene names of WC1-nd1 and WC1-nd2, respectively. The identification of a cDNA sequence lacking a corresponding genomic sequence could be a consequence of a gap in the genome sequence thus necessarily precluding the annotation of the corresponding gene. There is also evidence for copy number variation of WC1 genes among animals (G. Liu and J. Keele, personal communication, December 7, 2007) and this would also account for the observed differences.
Prior to these studies WC1 sequence corresponding to swine WC1 had not been identified in ruminants. The bovine swine-like WC1-11 reported here is structurally similar to WC1 in swine containing 6 SRCR domains, a transmembrane region and a long intracytoplasmic tail. It has been suggested that swine WC1 is the primitive version of its ruminant ortholog , so it could also be reasoned that in bovine WC1-11 is the most primitive of the ruminant WC1 genes. However, interestingly, the bovine WC1-11 has a very long intracytoplasmic region while swine WC1 genes have intracytoplasmic regions that are approximately the same length as those of the more classical bovine WC1 genes despite much dissimilarity in sequence . Current evidence for the classical bovine WC1 intracytoplasmic tails indicates that both tyrosine and serine phosphorylation is important for activation signals and endocytosis, respectively . It is possible that the presence of a tyrosine kinase phosphorylation motif within the unique portion of bovine WC1-11 intracytoplasmic region could result in a signaling and/or functional role that is distinct from other WC1 genes.
With regard to this, Wijngaard and co-workers identified and designated three distinct WC1 gene products as WC1.1, WC1.2 and WC1.3 based on reactivity with specific mAbs using WC1-transfected cells . Based on those studies, WC1 bearing γδT cells were subsequently defined based on mAb reactivity as WC1.1+, WC1.2+, WC1.1+/WC1.2+ or WC1.1+/WC1.3+ wherein the WC1.3+ population is only found as a subpopulation of WC1.1+ cells. While the sequence for WC1.1 has been reported in its entirety, only limited putative amino acid sequence for Domains 1 and 2 and nucleotide sequence for segments of the intracytoplasmic tails for WC1.2 and WC1.3 have been reported. WC1.3 was unique with regard to its long intracytoplasmic tail  and that sequence can be found to correspond to Type II WC1 tail sequences here. However, based on our annotations no WC1 gene was identified that had the sequence reported by Wijngaard et al.  as that corresponding to Domain 1 of WC1.3; in fact similar sequence was instead found in Domain 6 of WC1-4 and WC1-9, and thus we suggest part of the published WC1.3 sequence is erroneous. Despite this problem it has already been shown that functionally distinct subpopulations of bovine γδT cells can be defined based on the presence of particular WC1 molecules that react with monoclonal antibodies recognizing WC1.1 or WC1.2 [2, 14] and we now know that WC1 intracytoplasmic tails corresponding to the archetypal WC1.1 sequence play a critical role in signal transduction in response to antigen . Thus, it is important to further evaluate the role of the long intracytoplasmic tail regions contained in Type II genes WC1- 9, WC1-10, WC1-12 and the swine-like Type III gene WC1-11. Because Domain 1 is the most diverse among WC1 SRCR domains, as shown here, it is possible that it serves as the pattern recognition portion of the WC1 molecule and could be a region where bacterial products are ligated as occurs for DMBT1 . Therefore, pairings of particular WC1 Domain 1's with particular intracytoplasmic tail regions may be crucial to directing γδT cell responses and functions. Future studies will be targeted towards better understanding those relationships. For instance, transfection experiments with WC1-4 and WC1-9 would enable us to determine whether they bind the same ligands but their intracytoplasmic tails send different signals and thus result in differing functional responses.
Finally, the occurrence of a large variety of bovine WC1 molecules can be explained only in part by the number of WC1 genes since here we report evidence for extensive alternative splicing of bovine WC1 transcripts. In fact, all but one of the expressed WC1 genes we identified had corresponding splice variants. Immunoprecipitation of γδT cell membranes with anti-WC1 mAb results in a variety of bands including 144, 180, 200, 220, 240 and 300 kDa [10, 12, 53–57], lending support to the occurrence of multiple isoforms and/or swine-like WC1-11 on γδT cells. While the possibility remains that what appear to be alternative splice variants are instead genes that were not identified during the annotation process as a result of gaps in the genomic sequence, all but two alternative splice variants can be related to unspliced transcript sequences that are ≥ 98% identical. Moreover, previous reports indicate that swine and sheep WC1 orthologs [29, 46] as well as other SRCR family immune system molecules (i.e. CD5, CD6 and CD163) produce transcripts that are alternatively spliced [22–27]. However, interestingly, unlike for CD6 and CD163 [23–26], WC1 intracytoplasmic tail length appears to be dictated by the particular gene encoding a transcript and not by alternative splicing.
It is notable that Domain 1, the putative ligand-binding portion, was never found to be missing as a result of alternative splicing. While this could be an artifact due to primer design, the forward primer was designed to anneal in the leader sequence and thus that explanation is unlikely. Precedence for multiple isoforms of a T cell co-receptor is shown by the two CD4-like genes in fish which differ from each other structurally [58, 59]. The function of these smaller WC1 molecules with apparently intact Domain 1's and intracytoplasmic tails is unknown but intriguing. Because WC1 serves as a co-receptor on γδT cells, smaller WC1 molecules may be better able to co-cluster in the immune synapse with the shorter TCR chains (each being about 30 kDa). It is also possible that WC1 isoforms differ in their flexibility given that full-length WC1 molecules contain inter-domain or "hinge" regions following SRCR domains 3, 8, and 10, and this could affect interaction with the TCR. Differences in flexibility have been noted for functionally different immunoglobulin heavy chains with IgE and IgM lacking hinge regions. It is yet to be determined whether transcripts of the same gene but with different alternative splice variants are found expressed by an individual cell but perhaps WC1 splicing is initiated following interaction with its ligand.
Based on annotations of the bovine genome we identified 13 members of the WC1 gene family and their organization within two loci. Many of those genes had not been previously described, including a gene coding for a putatively more primitive and smaller swine-like WC1 molecule. Furthermore, we provide cDNA evidence as verification for many of the annotated sequences as well as evidence for isoforms derived by alternative splicing and the suggestion of at least two more WC1 genes. It is possible that WC1 diversity contributes to functional differences that have been observed between γδT cell subpopulations and here we have demonstrated that diversity of WC1 molecules is attributable to a large, multi-gene family as well as to the fact that WC1 transcripts undergo extensive alternative splicing.
In conjunction with the Bovine Genome Sequencing Consortium http://genomes.arc.georgetown.edu/bovine/, manual annotation of the WC1 genes was performed using the Apollo Genome Annotation and Curation Tool, version 1.6.5  and the bovine genome assembly Btau_3.1 . Predicted gene models of putative WC1 genes were identified by performing a BLAST search of archetypal WC1.1 sequence against the Bovine Official Gene Set (called GLEAN). Predicted gene models were then analyzed using the Apollo software and the following actions were performed when necessary based on available EST or cDNA evidence: (i) models were checked for correct exon-intron structure, (ii) initiation and termination codons were identified, (iii) exons were either added or deleted if it was determined that the coding region in the predicted model was incorrect and (iv) predicted gene models were split when a single model encompassed more than one gene or merged when two models coded for a single gene. Predicted gene models identified from the BLAST search were considered pseudogenes when premature stop codons, frameshifts and/or a WC1 coding region exceeding approximately 60 kb occurred in areas where the sequence integrity was deemed adequate.
Animals and cells
Blood was collected via jugular venipuncture into heparin from a single female Belted Galloway between the ages of 11 and 19 months. Animal use complied with federal guidelines and had IACUC approval. Peripheral blood mononuclear cells (PBMC) were isolated via density gradient centrifugation over Ficoll-Paque™ PLUS (GE Healthcare Bio-Sciences, Piscataway, NJ) according to the manufacturer's protocol. PBMC were cultured at 2.5 × 106 cells/ml with Concavalin A (ConA; 1.0 μg/ml; Sigma-Aldrich, St. Louis, MO) or leptospira antigen (, 0.5 μg/ml; sonicated whole cells of L. borgpetersenii serovar hardjo clone RZ33) in RPMI 1640 medium containing 10% heat-inactivated fetal bovine serum (HyClone, Logan, UT), 2 mM L-glutamine, 50 μM 2-mercaptoethanol and 50 μg/ml gentamicin at 37°C with 5% CO2 in air for six days.
RNA isolation and RT-PCR
Pelleted ex vivo, ConA-activated or leptospira antigen-activated cells were resuspended in TRIzol (Invitrogen, Carlsbad, CA) and RNA was isolated according to the manufacturer's protocol. Reverse transcription (RT) was performed using 1 μg of total RNA, oligo dT primers and AMV reverse transcriptase (AMV RT kit; Promega, Madison, WI). 2 μl of pooled cDNA was used as template in subsequent polymerase chain reactions (PCR) using the Elongase Amplification system (Invitrogen) and a final concentration of 1.5 mM Mg2+. Generally, PCR was conducted using a forward primer (designated "WC1atg-for") designed to amplify all WC1 transcripts based on the annotations described here and an additional forward primer (designated "WC1full-forA") was used in separate reactions to preferentially amplify archetypal WC1.1 transcripts. Reverse primers (designated WC1groups1,2-rev, WC1group3-rev and WC1group4-rev) were designed to specifically amplify each of three sequence-related subgroups of WC1 transcripts based on the WC1 genes identified here. Primer sequences are in Table 2. Cycling parameters were 30s at 94°C, 30s at 52°C and 4 m 30s at 68°C for 35 cycles for all reactions. In a limited number of cases, WC1 Domain 1 only was amplified in PCR reactions using the WC1atg-for primer and a reverse primer which was designed within a conserved region to amplify Domain 1 of all known WC1 molecules, designated SRCR1-rev (Table 2). Cycling parameters for those reactions were 30s at 95°C, 1 m at 58°C and 1 m at 72°C for 30 cycles with an expected band size of approximately 530 bp. PCR products were analyzed on 1.2% TAE agarose gels, visualized using SYBR Safe (Invitrogen) or ethidium bromide and cloned into the pCR2.1 vector (Invitrogen) or the pCR-XL vector (Invitrogen) according to the manufacturer's protocol. A total of 35 full-length cDNA clones were sequenced commercially (GeneWiz, South Plainfield, NJ) in order to verify the insert identity and for subsequent sequence analysis.
Sequencing of plasmids containing full-length WC1 cDNA was initially performed using T7 forward and M13 reverse primers. Subsequent sequencing reactions were performed using internal primers designed based on individual plasmid sequences (Table 2). Typically, four to six sequencing reactions were required to fully sequence a single WC1 transcript in the forward and reverse directions. Nucleotide sequences were aligned and consensus sequences were created using BioEdit version 188.8.131.52 . WC1 sequences reported here that were derived from cloned RT-PCR products were submitted to GenBank (http://www.ncbi.nlm.nih.gov/Genbank/index.html see Table 5 for accession numbers), and classified based on annotated genomic sequences described herein. Other sequences used for comparisons in analyses included bovine archetypal WC1.1 (GenBank accession number X63723) and swine WC1 (GenBank accession numbers CAA67706 to CAA67710). Exon-intron structure schematics were based on alignments of cDNA and genomic DNA sequence using SIM4  and visualized with LalnView http://pbil.univ-lyon1.fr/software/lalnview.html.
Multiple sequence alignments were performed using ClustalW2 (http://www.ebi.ac.uk/Tools/clustalw2/index.html; ) and the default parameters, refined by hand when necessary, and were visualized using JalView . SRCR domain organization of alternatively spliced variants was determined using ClustalW2 analysis of deduced amino acid sequences to determine which domains were represented in each clone. Phylogenetic analyses were performed using deduced amino acid sequences of individual WC1 domains or tails, as indicated. Phylogenies were constructed in MEGA4  using the neighbour-joining method  and the p-distance model for amino acid sequences and were tested using bootstrap analysis  with 1000 replications.
This work was conducted as part of the Bovine Genome Annotation Sequencing Consortium. The work was supported by USDA-NRI grants nos. 2006-01691 and 2005-01812 and CSREES-USDA Massachusetts Agricultural Experiment Station under project no. MAS00913 and Multi-State NRSP-008 project no. MAS00955.
- Naiman BM, Alt D, Bolin CA, Zuerner R, Baldwin CL: Protective killed Leptospira borgpetersenii vaccine induces potent Th1 immunity comprising responses by CD4 and gammadelta T lymphocytes. Infect Immun. 2001, 69: 7550-7558. 10.1128/IAI.69.12.7550-7558.2001.PubMed CentralView ArticlePubMed
- Rogers AN, Vanburen DG, Hedblom EE, Tilahun ME, Telfer JC, Baldwin CL: Gammadelta T cell function varies with the expressed WC1 coreceptor. J Immunol. 2005, 174: 3386-3393.View ArticlePubMed
- Carding SR, Egan PJ: Gammadelta T cells: functional plasticity and heterogeneity. Nat Rev Immunol. 2002, 2: 336-345. 10.1038/nri797.View ArticlePubMed
- Hayday A, Tigelaar R: Immunoregulation in the tissues by gammadelta T cells. Nat Rev Immunol. 2003, 3: 233-242. 10.1038/nri1030.View ArticlePubMed
- Blumerman SL, Herzig CT, Baldwin CL: WC1+ gammadelta T cell memory population is induced by killed bacterial vaccine. Eur J Immunol. 2007, 37: 1204-1216. 10.1002/eji.200636216.View ArticlePubMed
- Chen ZW, Letvin NL: Adaptive immune response of Vgamma2Vdelta2 T cells: a new paradigm. Trends Immunol. 2003, 24: 213-219. 10.1016/S1471-4906(03)00032-2.PubMed CentralView ArticlePubMed
- Shen Y, Zhou D, Qiu L, Lai X, Simon M, Shen L, Kou Z, Wang Q, Jiang L, Estep J, et al: Adaptive immune response of Vgamma2Vdelta2+ T cells during mycobacterial infections. Science. 2002, 295: 2255-2258. 10.1126/science.1068819.PubMed CentralView ArticlePubMed
- Clevers H, MacHugh ND, Bensaid A, Dunlap S, Baldwin CL, Kaushal A, Iams K, Howard CJ, Morrison WI: Identification of a bovine surface antigen uniquely expressed on CD4-CD8- T cell receptor gamma/delta+ T lymphocytes. Eur J Immunol. 1990, 20: 809-817. 10.1002/eji.1830200415.View ArticlePubMed
- Mackay CR, Beya MF, Matzinger P: Gamma/delta T cells express a unique surface molecule appearing late during thymic development. Eur J Immunol. 1989, 19: 1477-1483. 10.1002/eji.1830190820.View ArticlePubMed
- Morrison WI, Davis WC: Individual antigens of cattle. Differentiation antigens expressed predominantly on CD4- CD8- T lymphocytes (WC1, WC2). Vet Immunol Immunopathol. 1991, 27: 71-76. 10.1016/0165-2427(91)90082-N.View ArticlePubMed
- Wijngaard PL, Metzelaar MJ, MacHugh ND, Morrison WI, Clevers HC: Molecular characterization of the WC1 antigen expressed specifically on bovine CD4-CD8- gamma delta T lymphocytes. J Immunol. 1992, 149: 3273-3277.PubMed
- Mackay CR, Maddox JF, Brandon MR: Three distinct subpopulations of sheep T lymphocytes. Eur J Immunol. 1986, 16: 19-25. 10.1002/eji.1830160105.View ArticlePubMed
- Carr MM, Howard CJ, Sopp P, Manser JM, Parsons KR: Expression on porcine gamma delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma delta T lymphocytes. Immunology. 1994, 81: 36-40.PubMed CentralPubMed
- Lahmers KK, Norimine J, Abrahamsen MS, Palmer GH, Brown WC: The CD4+ T cell immunodominant Anaplasma marginale major surface protein 2 stimulates gammadelta T cell clones that express unique T cell receptors. J Leukoc Biol. 2005, 77: 199-208. 10.1189/jlb.0804482.View ArticlePubMed
- Lozano F, Simarro M, Calvo J, Vila JM, Padilla O, Bowen MA, Campbell KS: CD5 signal transduction: positive or negative modulation of antigen receptor signaling. Crit Rev Immunol. 2000, 20: 347-358.View ArticlePubMed
- Patel DD, Wee SF, Whichard LP, Bowen MA, Pesando JM, Aruffo A, Haynes BF: Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells. J Exp Med. 1995, 181: 1563-1568. 10.1084/jem.181.4.1563.View ArticlePubMed
- Bott CM, Doshi JB, Morimoto C, Romain PL, Fox DA: Activation of human T cells through CD6: functional effects of a novel anti-CD6 monoclonal antibody and definition of four epitopes of the CD6 glycoprotein. Int Immunol. 1993, 5: 783-792. 10.1093/intimm/5.7.783.View ArticlePubMed
- Gangemi RM, Swack JA, Gaviria DM, Romain PL: Anti-T12, an anti-CD6 monoclonal antibody, can activate human T lymphocytes. J Immunol. 1989, 143: 2439-2447.PubMed
- Swack JA, Gangemi RM, Rudd CE, Morimoto C, Schlossman SF, Romain PL: Structural characterization of CD6: properties of two distinct epitopes involved in T cell activation. Mol Immunol. 1989, 26: 1037-1049. 10.1016/0161-5890(89)90068-0.View ArticlePubMed
- Sulahian TH, Hogger P, Wahner AE, Wardwell K, Goulding NJ, Sorg C, Droste A, Stehling M, Wallace PK, Morganelli PM, Guyre PM: Human monocytes express CD163, which is upregulated by IL-10 and identical to p155. Cytokine. 2000, 12: 1312-1321. 10.1006/cyto.2000.0720.View ArticlePubMed
- Schaer DJ, Alayash AI, Buehler PW: Gating the radical hemoglobin to macrophages: the anti-inflammatory role of CD163, a scavenger receptor. Antioxid Redox Signal. 2007, 9: 991-999. 10.1089/ars.2007.1576.View ArticlePubMed
- Ligtenberg AJ, Veerman EC, Nieuw Amerongen AV, Mollenhauer J: Salivary agglutinin/glycoprotein-340/DMBT1: a single molecule with variable composition and with different functions in infection, inflammation and cancer. Biol Chem. 2007, 388: 1275-1289. 10.1515/BC.2007.158.View ArticlePubMed
- Bowen MA, Whitney GS, Neubauer M, Starling GC, Palmer D, Zhang J, Nowak NJ, Shows TB, Aruffo A: Structure and chromosomal location of the human CD6 gene: detection of five human CD6 isoforms. J Immunol. 1997, 158: 1149-1156.PubMed
- Padilla O, Calvo J, Vila JM, Arman M, Gimferrer I, Places L, Arias MT, Pujana MA, Vives J, Lozano F: Genomic organization of the human CD5 gene. Immunogenetics. 2000, 51: 993-1001. 10.1007/s002510000235.View ArticlePubMed
- Robinson WH, Neuman de Vegvar HE, Prohaska SS, Rhee JW, Parnes JR: Human CD6 possesses a large, alternatively spliced cytoplasmic domain. Eur J Immunol. 1995, 25: 2765-2769. 10.1002/eji.1830251008.View ArticlePubMed
- Law SK, Micklem KJ, Shaw JM, Zhang XP, Dong Y, Willis AC, Mason DY: A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. Eur J Immunol. 1993, 23: 2320-2325. 10.1002/eji.1830230940.View ArticlePubMed
- Ritter M, Buechler C, Langmann T, Schmitz G: Genomic organization and chromosomal localization of the human CD163 (M130) gene: a member of the scavenger receptor cysteine-rich superfamily. Biochem Biophys Res Commun. 1999, 260: 466-474. 10.1006/bbrc.1999.0866.View ArticlePubMed
- Nielsen MJ, Madsen M, Moller HJ, Moestrup SK: The macrophage scavenger receptor CD163: endocytic properties of cytoplasmic tail variants. J Leukoc Biol. 2006, 79: 837-845. 10.1189/jlb.1005602.View ArticlePubMed
- Kanan JH, Nayeem N, Binns RM, Chain BM: Mechanisms for variability in a member of the scavenger-receptor cysteine-rich superfamily. Immunogenetics. 1997, 46: 276-282. 10.1007/s002510050273.View ArticlePubMed
- O'Keeffe MA, Metcalfe SA, Cunningham CP, Walker ID: Sheep CD4(+) alphabeta T cells express novel members of the T19 multigene family. Immunogenetics. 1999, 49: 45-55. 10.1007/s002510050462.View ArticlePubMed
- Gronlund J, Vitved L, Lausen M, Skjodt K, Holmskov U: Cloning of a novel scavenger receptor cysteine-rich type I transmembrane molecule (M160) expressed by human macrophages. J Immunol. 2000, 165: 6406-6415.View ArticlePubMed
- Mollenhauer J, Wiemann S, Scheurlen W, Korn B, Hayashi Y, Wilgenbus KK, von Deimling A, Poustka A: DMBT1, a new member of the SRCR superfamily, on chromosome 10q25.3-26.1 is deleted in malignant brain tumours. Nat Genet. 1997, 17: 32-39. 10.1038/ng0997-32.View ArticlePubMed
- Hao L, Nei M: Genomic organization and evolutionary analysis of Ly49 genes encoding the rodent natural killer cell receptors: rapid evolution by repeated gene duplication. Immunogenetics. 2004, 56: 343-354. 10.1007/s00251-004-0703-0.View ArticlePubMed
- Wilhelm BT, Mager DL: Rapid expansion of the Ly49 gene cluster in rat. Genomics. 2004, 84: 218-221. 10.1016/j.ygeno.2004.01.010.View ArticlePubMed
- Barten R, Trowsdale J: The human Ly-49L gene. Immunogenetics. 1999, 49: 731-734. 10.1007/s002510050675.View ArticlePubMed
- Westgaard IH, Berg SF, Orstavik S, Fossum S, Dissen E: Identification of a human member of the Ly-49 multigene family. Eur J Immunol. 1998, 28: 1839-1846. 10.1002/(SICI)1521-4141(199806)28:06<1839::AID-IMMU1839>3.0.CO;2-E.View ArticlePubMed
- McQueen KL, Wilhelm BT, Harden KD, Mager DL: Evolution of NK receptors: a single Ly49 and multiple KIR genes in the cow. Eur J Immunol. 2002, 32: 810-817. 10.1002/1521-4141(200203)32:3<810::AID-IMMU810>3.0.CO;2-P.View ArticlePubMed
- Martin AM, Freitas EM, Witt CS, Christiansen FT: The genomic organization and evolution of the natural killer immunoglobulin-like receptor (KIR) gene cluster. Immunogenetics. 2000, 51: 268-280. 10.1007/s002510050620.View ArticlePubMed
- Wilson MJ, Torkar M, Haude A, Milne S, Jones T, Sheer D, Beck S, Trowsdale J: Plasticity in the organization and sequences of human KIR/ILT gene families. Proc Natl Acad Sci USA. 2000, 97: 4778-4783. 10.1073/pnas.080588597.PubMed CentralView ArticlePubMed
- Guethlein LA, Flodin LR, Adams EJ, Parham P: NK cell receptors of the orangutan (Pongo pygmaeus): a pivotal species for tracking the coevolution of killer cell Ig-like receptors with MHC-C. J Immunol. 2002, 169: 220-229.View ArticlePubMed
- Hershberger KL, Shyam R, Miura A, Letvin NL: Diversity of the killer cell Ig-like receptors of rhesus monkeys. J Immunol. 2001, 166: 4380-4390.View ArticlePubMed
- Gagnier L, Wilhelm BT, Mager DL: Ly49 genes in non-rodent mammals. Immunogenetics. 2003, 55: 109-115.PubMed
- Nylenna O, Naper C, Vaage JT, Woon PY, Gauguier D, Dissen E, Ryan JC, Fossum S: The genes and gene organization of the Ly49 region of the rat natural killer cell gene complex. Eur J Immunol. 2005, 35: 261-272. 10.1002/eji.200425429.View ArticlePubMed
- Blumerman SL, Herzig CT, Rogers AN, Telfer JC, Baldwin CL: Differential TCR gene usage between WC1- and WC1+ ruminant gammadelta T cell subpopulations including those responding to bacterial antigen. Immunogenetics. 2006, 58: 680-692. 10.1007/s00251-006-0122-5.View ArticlePubMed
- Wijngaard PL, MacHugh ND, Metzelaar MJ, Romberg S, Bensaid A, Pepin L, Davis WC, Clevers HC: Members of the novel WC1 gene family are differentially expressed on subsets of bovine CD4-CD8- gamma delta T lymphocytes. J Immunol. 1994, 152: 3476-3482.PubMed
- O'Keeffe MA, Metcalfe SA, Glew MD, Bowden T, McInnes S, Kimpton WG, Cahill RN, Hein WR, Walker ID: Lymph node homing cells biologically enriched for gamma delta T cells express multiple genes from the T19 repertoire. Int Immunol. 1994, 6: 1687-1697. 10.1093/intimm/6.11.1687.View ArticlePubMed
- Walker ID, Glew MD, O'Keeffe MA, Metcalfe SA, Clevers HC, Wijngaard PL, Adams TE, Hein WR: A novel multi-gene family of sheep gamma delta T cells. Immunology. 1994, 83: 517-523.PubMed CentralPubMed
- Rogers AN, Vanburen DG, Zou B, Lahmers KK, Herzig CT, Brown WC, Telfer JC, Baldwin CL: Characterization of WC1 co-receptors on functionally distinct subpopulations of ruminant gamma delta T cells. Cell Immunol. 2006, 239: 151-161. 10.1016/j.cellimm.2006.05.006.View ArticlePubMed
- Wang F, Herzig C, Ozer D, Baldwin CL, Telfer JC: Tyrosine phosphorylation of scavenger receptor cysteine-rich WC1 is required for the WC1-mediated potentiation of TCR-induced T-cell proliferation. Eur J Immunol. 2009, 39: 254-266. 10.1002/eji.200838472.View ArticlePubMed
- Barclay AN, Hatherley D: The counterbalance theory for evolution and function of paired receptors. Immunity. 2008, 29: 675-678. 10.1016/j.immuni.2008.10.004.PubMed CentralView ArticlePubMed
- Vaccarelli G, Miccoli MC, Antonacci R, Pesole G, Ciccarese S: Genomic organization and recombinational unit duplication-driven evolution of ovine and bovine T cell receptor gamma loci. BMC Genomics. 2008, 9: 81-10.1186/1471-2164-9-81.PubMed CentralView ArticlePubMed
- Madsen J, Tornoe I, Nielsen O, Lausen M, Krebs I, Mollenhauer J, Kollender G, Poustka A, Skjodt K, Holmskov U: CRP-ductin, the mouse homologue of gp-340/deleted in malignant brain tumors 1 (DMBT1), binds gram-positive and gram-negative bacteria and interacts with lung surfactant protein D. Eur J Immunol. 2003, 33: 2327-2336. 10.1002/eji.200323972.View ArticlePubMed
- Jones WM, Walcheck B, Jutila MA: Generation of a new gamma delta T cell-specific monoclonal antibody (GD3.5). Biochemical comparisons of GD3.5 antigen with the previously described Workshop Cluster 1 (WC1) family. J Immunol. 1996, 156: 3772-3779.PubMed
- Crocker G, Sopp P, Parsons K, Davis WC, Howard CJ: Analysis of the gamma/delta T cell restricted antigen WC1. Vet Immunol Immunopathol. 1993, 39: 137-144. 10.1016/0165-2427(93)90174-3.View ArticlePubMed
- Davis BD: Limits to genetic intervention in humans: somatic and germline. Ciba Found Symp. 1990, 149: 81-86. discussion 87–92.PubMed
- Davis WC, Marusic S, Lewin HA, Splitter GA, Perryman LE, McGuire TC, Gorham JR: The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species. Vet Immunol Immunopathol. 1987, 15: 337-376. 10.1016/0165-2427(87)90005-5.View ArticlePubMed
- Morrison WI, MacHugh ND, Bensaid A, Goddeeris BM, Teale AJ, McKeever DJ: A monoclonal antibody which reacts specifically with a population of bovine lymphocytes lacking B cell and T cell markers. Adv Exp Med Biol. 1988, 237: 591-596.View ArticlePubMed
- Dijkstra JM, Somamoto T, Moore L, Hordvik I, Ototake M, Fischer U: Identification and characterization of a second CD4-like gene in teleost fish. Mol Immunol. 2006, 43: 410-419. 10.1016/j.molimm.2005.03.005.View ArticlePubMed
- Laing KJ, Zou JJ, Purcell MK, Phillips R, Secombes CJ, Hansen JD: Evolution of the CD4 family: teleost fish possess two divergent forms of CD4 in addition to lymphocyte activation gene-3. J Immunol. 2006, 177: 3939-3951.View ArticlePubMed
- Lewis SE, Searle SM, Harris N, Gibson M, Lyer V, Richter J, Wiel C, Bayraktaroglir L, Birney E, Crosby MA, et al: Apollo: a sequence annotation editor. Genome Biol. 2002, 3: RESEARCH0082-10.1186/gb-2002-3-12-research0082.PubMed CentralView ArticlePubMed
- Elsik CG, Tellam RL, Worley KC: The Genome sequence of taurine cattle: A window to ruminant biology and evolution. Science. 2009, 324 (5926): 522-528. 10.1126/science.1169588.PubMed CentralView ArticlePubMed
- Hall TA: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser. 1999, 41: 95-98.
- Florea L, Hartzell G, Zhang Z, Rubin GM, Miller W: A computer program for aligning a cDNA sequence with a genomic DNA sequence. Genome Res. 1998, 8: 967-974.PubMed CentralPubMed
- Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, et al: Clustal W and Clustal X version 2.0. Bioinformatics. 2007, 23: 2947-2948. 10.1093/bioinformatics/btm404.View ArticlePubMed
- Clamp M, Cuff J, Searle SM, Barton GJ: The Jalview Java alignment editor. Bioinformatics. 2004, 20: 426-427. 10.1093/bioinformatics/btg430.View ArticlePubMed
- Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24: 1596-1599. 10.1093/molbev/msm092.View ArticlePubMed
- Saitou N, Nei M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987, 4: 406-425.PubMed
- Felsenstein J: Confidence limits on phylogenies: An approach using the bootstrap. Evolution. 1985, 39: 783-791. 10.2307/2408678.View Article
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