Next-generation pyrosequencing of gonad transcriptomes in the polyploid lake sturgeon (Acipenser fulvescens): the relative merits of normalization and rarefaction in gene discovery

Table 1 Number of reads and nucleotides produced by five cDNA libraries (L1–L5). Significant BLAST hit criteria were an e-value ≤ 1 × 10^-3 and a bit score > 40.

	L1	L2	L3	L4	L5	Total
Tissue source	adolescent male	adolescent female	unk. juvenile	adolescent male	adolescent female	n/a
Status	normalized	normalized	native	native	native	n/a
N. reads	3,811	13,414	11,178	10,296	8,361	47,060
N. reads after trimming	1,234	1,385	8,700	5,061	4,361	20,741
N. nucleotides	286,822	270,099	1,247,400	870,023	869,912	3,544,256
Mean read length	232	195	143	162	199	186
Median read length	251	218	133	170	231	201
Mode read length	243	261	100	75	240	184
Significant BLAST hits	241	262	1,926	996	1,585	5,010

ISSN: 1471-2164