Table 1 Gene specific PCR primers to amplify 16 serpins that have basic residues at their P1 site
Serpin gene | Forward primer | Reverse |
|---|---|---|
S1 | CAAAGCTGATACAAGACAAGACCAAG | TTAAAGATTATTGACCTGTCCAGCG |
S2 | AGTGAAGGAACGAAGAGCTCGAC | TTGTGGAGACATCTGAGATCTTCAGA |
S4 | AACAGCGATGGGAATGGTTTTC | TCGTTGGGCACAGTTAGGGTC |
S6 | TACCAAGATGAAAGTTCTCGTGACG | CTTGGCTCAAAGGTGGTTCACTT |
S7 | ATGAAAGTTATCACTGCGTTCCTGT | CTTGGTTGTCTCAGAGGTGGTTCA |
S8 | TCAAACCTTGTTTACTTGCCCCA | TCAAACCTTGTTTACTTGCCCCA |
S9 | TCTTCTCGCCCTACAGCATATCC | TGTTTTGAAGCCTGGCGATTGT |
S12 | ATGTTCGCTCCGACTGCCCT | GTTAATTTTTCACGTTCTCAAGCCTG |
S14 | ATGAAGGCTTCAACGGTGTGG | GGTAATAAAAGTGGTCGCACACAATG |
S16 | GGTGAGTCAAGATGGCAAAATGCC | GTTTACCTGTCCTGCAAACAGCACC |
S17 | GAGACGAGACAGACCAATTACCTCA | GGATGACTCAGAGGGCGTTGAT |
S18 | TATCACAACCTCCACGATGTTCG | GTTAATTTCTCACGTTCTCAAGCCTG |
S19 | ATGTGGTTCCCGGCGCTCCT | CGTCCTCGATGTATTCACAAGTCCAGC |
S21 | CTGTTCCTCCTGATGGCGGTA | ATTTCTCACGTTCTCAAGCCTGC |
S26 | ATGCTTGCGGAAAAGGTTTTC | CTGGACTTCTCCCAAAAATAGCG |
S27 | AAGCTAGATCGACGCCATGAAG | CTAAACCCGCTTCAACATGCTG |
S32 | GCATCCAGAGCTTCCAAACATG | TCAATGTGATAGGAAGCACTTCGAG |
Tick Actin | GGACAGCTACGTGGGCGACGAGG | CGATTTCACGCTCAGCCGTGGTGG |