Table 5 Primer sequences used in real-time PCR expression analysis for B. thermophilus SSH libraries validation

Elongation factor beta

For: 5' GATCTTAAAAGTAAAGCTGGTCAGCAAGC 3'

Rev: 5' AACAAATCAAAATCATCATCATCACCGCC 3'

Ribosomal protein L3

For: 5' AGATATATCGTATTGGAGAGGGATACCACACC 3'

Rev: 5' GCTGTTGCATCCTTCTTCAATGGACCCAT 3'

Foot protein

For: 5' AATAATGGTAAATGTGTTGCTAATGGCTA 3'

Rev: 5' CCGTATCCCCTTCTACAACATCTACCGCC 3'

Pedal retractor myosin

For: 5' AGAACCGACGAATTGGAAGAGGCCAAGAG 3'

Rev: 5' AACAATTCAGCAGAGTAACTGCGGGCCTC 3'

Adhesive plaque matrix

For: 5' AAAAGATGTGAAGTAAACAGATGCAGCCCA 3'

Rev: 5' CCGTATCCCCTTCTACAACATCTGCCGCC 3'

Actin

For: 5' ACGCGGGTCAGGGTCGGACGTAGCCACGC 3'

Rev: 5' ATGGAGATCAGACGGAGATGGTCCTCCTC 3'

Adenosylhomocysteinase

For: 5' GTAAATCTTGGTTGTGCTCATGGTCATCC 3'

Rev: 5' GATTTGAATGGTCCTTCTTTAGGTAGACC 3'

Cyclophilin S

For: 5' TTGAATAAAGCCAGATGGATGGATGGAAA 3'

Rev:5' AAATCTTCATCGCTAGCTGCTTGTGCTTC 3'

Arginine kinase

For: 5' ATGGGTGAAGTAGCAGAATTGTGGGCTAA 3'

Rev: 5' TCACATGCATACAATCCGACTCCGCT 3'

Glutathione peroxidase

For: 5' ATGGGCATAAACTTGGGAGACATTTT 3'

Rev: 5' CCTAATTCTGTTGTACAAACAGGGGTATA 3'

BthermEST1

For: 5' AGTGACTTCACAACTGCCCGTATGTGGAA 3'

Rev:5' TTGGTGCACATCATCAAGAAGGAGAGTAT 3'

Kalicludine

For: 5' CCATGCAATGAAGATTGTCTTTTGCCAAA 3'

Rev: 5' TTTGGCAAAAGACAATCTTCATTGCATGG 3'

Defensin

For: 5' ATGTTCAAAGTAACTTTGTTCTTCGTTGG 3'

Rev: 5' TACGATCTGCAGTGACACACGTTATG 3'

SPARC

For: 5' AACGCAGACGACCACCGTACAGACGC 3'

Rev: 5' TATGCATCACACTTGTCTGTAATGTCAACC 3'

Δ5-desaturase

For: 5' AACGACTGGTTTACAGGGCATCTAAA 3'

Rev: 5' TTTAGATGCCCTGTAAACCAGTCGTT 3'

Carbonic anhydrase-2

For: 5' GATGACAAGGAAGGATCTGAGCACACTCT 3'

Rev: 5' AGAGTGTGCTCAGATCCTTCCTTGTCATC 3'

HSP90

For: 5' ATGCCTGAACCTGAAACAACTATGGATGA 3'

Rev: 5' GAATACATCTGGGAATCTGCAGCTGGTGG 3'

Myc homolog

For: 5' TCTGTTTATGATGCCTGGGTCACTCC 3'

Rev: 5' GGAGTGACCCAGGCATCATAAACAGA 3'

Cytosolic malate dehydrogenase

For: 5' ATGGCAGTTCCTTCAGATGGATCTTA 3'

Rev: 5' CAATACACAAAAACAGACACTGTATACAT 3'

Techylectin 5A

For: 5' GGATATCAGGGTAATGCAGGAGATGC 3'

Rev: 5' GCATCTCCTGCATTACCCTGATATCC 3'