Table 6 Primer sequences used in real-time PCR expression analysis for P. pandorae irlandei SSH libraries validation.

Intracellular hemoglobin

For: 5' CTTGCCGATAACATTACTGCTGTTCGAGG 3'

Rev: 5' TCGGCATCACCCGCCTTCTCCGCTACGTC 3'

Hemoglobin A2c

For: 5' GTTCTGATCATAATCGCTGTCTGTCTGG 3'

Rev: 5' TGTAGAAGCTGGGCATTCAGCGTGTCGGG 3'

Linker L1

For: 5' ATCATGGCAGGCCTGGTGGCACTCGCCAT 3'

Rev: 5' TCTGATCCGTCATGACAGTCGTTAGCACC 3'

Linker L2

For: 5' ATGGTTGACGATGAGATGGACTTGATGGA 3'

Rev: 5' ATCTTATAGCTGTCTATAGTAACCCG 3'

Hemoglobin B2

For: 5' CTGGATCATCTCGGCCGTCAGCATGTTGT 3'

Rev: 5' GTGTGGTCGAGACGCGTTGCTCGGTCCGC 3'

PpandEST 2

For: 5' CAACCATGTGCCTACTTTACCTTGTTCAG 3'

Rev: 5' AAGAACAGCTGCTGCAGAATATCTCCCAC 3'

Xylan endohydrolase

For: 5' GAGATGGAAGAGAGAAATCCAGATTGGCT 3'

Rev: 5' AGTTTCGCCTTCGAGTCACCGCTGTAGGC 3'

Rab 7

For: 5' CTTATTCAAGCTAGCCCACGAGATCCAGA 3'

Rev: 5' CAACTCTCCGCTGAAGTCTTCGCCCGGTC 3'

Adenosylhomocysteinase

For: 5' ATTGTGTGCAATATTGGACATTTTGACTGTGA 3'

Rev: 5' AGACCTAAATAGCCAGCCTGGTCATCTGA 3'

Chymotrypsin

For: 5' ACAGAGGTCGAATACGAGGTGATGACAAT 3'

Rev: 5' ATGCCACCTGAGCTAAGAGTTCCCCATCC 3'

PpandEST 1

For: 5' GAAGCTGACCTAGCTTACGCCGGTCTGAA 3'

Rev:5' TAGGGCTCGAGCGGCCGCCCGGGCAG 3'

Ribosomal protein S16

For: 5' GCTGTTGCTCACTGCAAACAGGGCAAAGGT 3'

Rev: 5' TTGATCTCTTTCTTTGATGCTTCGTCGTC 3'

Nidogen secreted domain protein

For: 5' CAATGCAAGTATTGGCCATGGCATGGTAG 3'

Rev: 5' ACCCAGCGTCCTGCTTTGGCGACGTTACT 3'

Lipid binding protein

For: 5' TTCAACATGTCTCAATTGAATGGGAAATGGAA 3'

Rev: 5' TCCAGGCCCGTTTCGTTCACCTCGATACG 3'