Figure 4

Anti-CD3- and anti-CD3/CD28-stimulations leading to Itk- and calcineurin-dependent and -independent transcriptional signatures. Three different groups of genes exist in each stimulation condition. The groups are Itk- and calcineurin-dependent (Itk/CN), Itk-dependent and calcineurin-independent (Itk/non-CN) and Itk-independent and calcineurin-dependent (non-Itk/CN). 113 probe-sets are overlapping between Itk/CN groups in the two stimulations. In each group the 10 most highly regulated transcripts are presented. All the chosen genes passed the t-test criterion of p < 0.05. The down-regulated transcripts are shown with "-". The transcripts in the two Itk/CN groups passed the criterion in at least one of the two comparisons. Genes previously known to be calcineurin-regulated are grey-shaded [16]. Arrows denoting signal transduction from CD28 have been omitted for clarity.