Systems level analysis of two-component signal transduction systems in Erwinia amylovora: Role in virulence, regulation of amylovoran biosynthesis and swarming motility

Table 1 TCSTs and other signal transduction genes in Erwinia amylovora and mutant construction

Gene/Operon^a	HK/RR/Hybrid/kinase^b	Mutants constructed^c
arcB, arcA	HK, RR	ΔarcB, ΔarcA
baeSR	HK, RR	ΔbaeS, ΔbaeR, ΔbaeSR
cpxA1R1	HK, RR	ΔcpxA1, ΔcpxR1, ΔcpxAR1
cpxA2R2	HK, RR	ΔcpxA2, ΔcpxR2, ΔcpxAR2
dcuSR	HK, RR	ΔdcuS, ΔdcuR, ΔdcuSR
envZ/ompR	HK, RR	ΔenvZ, ΔompR, ΔenvZ/ompR
grrS, grrA	HK hybrid, RR	ΔgrrS, ΔgrrA
hrpXY, hrpS	HK, RR, EBP	ΔhrpX, ΔhrpY, ΔhrpXY, ΔhrpXYS
kdpD, kdpE	HK, RR	ΔkdpD, ΔkdpE
narQP	HK, RR	ΔnarQ, ΔnarP, ΔnarQP
phoQP	HK, RR	ΔphoQ, ΔphoP, ΔphoQP
phoRB	HK, RR	ΔphoR, ΔphoB, ΔphoRB
pmrBA	HK hybrid, RR	ΔpmrB, ΔpmrA, ΔpmrBA
rcsCDB	HK hybrid, HPT, RR	ΔrcsD, ΔrcsB, ΔrcsC, ΔrcsBD
rstB, rstA	HK, RR	ΔrstB, ΔrstA
yehUT	HK, RR	ΔyehU, ΔyehT, ΔyehUT
yfhKA	HK, RR	ΔyfhK, ΔyfhA, ΔyfhKA
Other signal transduction genes used in this study ^b
eamIR	AHL, RR	ΔeamI, ΔeamR
luxQ, luxP	AI2 sensor kinase, RR	ΔluxQ, ΔluxP
Spk1	Serine kinase	Δspk1
ybjN	Kinase	ΔybjN
yciR	GGDEF-EAL	ΔyciR
yddV	GGDEF	ΔyddV
yegE	GGDEF	ΔyegE
yoaD	EAL	ΔyoaD

^a Genes for one TCST system are listed separately if they are not encoded within an operon in the genome. Otherwise, TCSTs are listed as an operon if genes are present together. Mutants for ntrBC were not constructed.
^b All TCSTs are sensor kinases and their corresponding DNA-binding response regulators. We included two sets of quorum sensing genes and four RRs with enzymatic output domain (EAL-GGDEF proteins) and two kinases. EBP: NtrC family of enhancing-binding protein. HPT: Histidine phosphotransfer domain; AHL: Acyl-homoserine lactone; GGDEF: Diguanylate cyclase; EAL: Type I c-di-GMP phosphodiesterase.
^c Mutant designation see Table 3.

ISSN: 1471-2164