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Table 3 E. amylovora TCTS mutants and their phenotypes

From: Systems level analysis of two-component signal transduction systems in Erwinia amylovora: Role in virulence, regulation of amylovoran biosynthesis and swarming motility

Gene/Operona

Mutant designation

Virulence assayb

Amylovoran production (48 h) (OD600)c

Significance levele

Distance of swarming motility (48 h, cm)d

Significance levele

WT

 

+++

0.08 ± 0.002

nop

C

2.7 ± 0.15

cde

ams

Z0118Δams

---

0.002 ± 0.0005

r

ND

ND

ND

flhD

Z2946ΔflhD

+++

ND

ND

C

0.7 ± 0

w

fliA

Z2986ΔfliA

+++

ND

ND

C

0.7 ± 0

w

arcA

Z1306ΔarcA

+++

0.053 ± 0.0001

qr

C

1.23 ± 0.09

uv

arcB

Z0117ΔarcB

+++

0.067 ± 0.0002

pq

C

1.83 ± 0.28

qr

baeR

Z3120ΔbaeR

+++

0.14 ± 0.045

klmn

I

1.87 ± 0.25

qr

baeS

Z3119ΔbaeS

+++

0.13 ± 0.03

klmn

I

2.07 ± 0.06

nopq

baeSR

Z3219–20ΔbaeSR

+++

0.1 ± 0.01

lmn

I

2.07 ± 0.06

nopq

cpxA1

Z0686ΔcpxA1

+++

0.09 ± 0.014

nop

I

1.40 ± 0.22

tu

cpxA1R1

Z0686–87ΔcpxAR1

+++

0.055 ± 0.003

pqr

I

1.33 ± 0.05

uv

cpxR1

Z0687ΔcpxR1

+++

0.08 ± 0.008

nop

I

1.16 ± 0.13

v

cpxA2

Z3367ΔcpxA2

+++

0.35 ± 0.023

hgi

I

2.5 ± 0.4

efghi

cpxA2R2

Z3367–68ΔcpxAR2

+++

0.29 ± 0.07

i

I

2.07 ± 0.34

opqr

cpxR2

Z3368ΔcpxR2

+++

0.35 ± 0.058

hgi

I

2.5 ± 0.14

efgh

dcuR

Z2361ΔdcuR

+++

0.198 ± 0.01

k

I

2.2 ± 0.17

ijklm

dcuS

Z2362ΔdcuS

+++

0.171 ± 0.02

kl

I

2.07 ± 0.32

ijklm

dcuSR

Z2361–62ΔdcuSR

+++

0.256 ± 0.02

j

I

2.23 ± 0.38

mnop

eamI

Z2195ΔeamI

+++

0.256 ± 0.046

j

I

2.23 ± 0.12

ijklm

eamR

Z2194ΔeamR

+++

0.544 ± 0.1

de

I

2.03 ± 0.31

pqr

envZ

Z0270ΔenvZ

+++

1.55 ± 0.06

c

C

1.03 ± 0.11

v

envZ/ompR

Z0270–71 ΔenvZ/ompR

+++

1.7 ± 0.05

a

C

1.03 ± 0.11

v

ompR

Z0271ΔompR

+++

1.63 ± 0.06

b

C

1.03 ± 0.11

v

grrA

Z2198ΔgrrA

+++

1.58 ± 0.15

bc

C

3.15 ± 0.07

a

grrS

Z3742ΔgrrS

+++

1.56 ± 0.09

c

C

3.2 ± 0.05

a

hrpX

Z3962ΔhrpX

+++

0.69 ± 0.11

d

I

1.73 ± 0.15

rs

hrpY

Z3963ΔhrpY

+++

0.471 ± 0.05

e

I

2.0 ± 0.0

opqr

hrpXY

Z3962–63 ΔhrpXY

+++

0.45 ± 0.09

ef

I

2.33 ± 0.15

hijkl

hrpXYS

Z3962–64 ΔhrpXYS

---

0.4 ± 0.09

gh

I

2.27 ± 0.15

hijkl

kdpD

Z0414ΔkdpD

+++

0.372 ± 0.02

hgi

I

2.13 ± 0.12

nopq

kdpE

Z1912ΔkdpE

+++

0.09 ± 0.035

no

I

1.43 ± 0.19

tu

luxP

Z3324ΔluxP

+++

0.419 ± 0.12

g

I

1.87 ± 0.23

rs

luxQ

Z3327ΔluxQ

+++

0.625 ± 0.11

de

I

1.9 ± 0.17

qr

narP

Z3390ΔnarP

+++

0.1 ± 0.001

lmn

I

2.2 ± 0.0

klmno

narQ

Z3389ΔnarQ

+++

0.08 ± 0.003

lmn

I

2.4 ± 0.1

fghij

narQP

Z3389–90 ΔnarQP

+++

0.08 ± 0.002

nop

I

2.27 ± 0.25

ghijkl

phoB

Z1675ΔphoB

+++

0.145 ± 0.003

klmn

I

2.6 ± 0.2

defg

phoR

Z1676ΔphoR

+++

0.366 ± 0.01

hgi

I

2.75 ± 0.15

cd

phoRB

Z1675–76 ΔphoRB

+++

0.312 ± 0.01

hi

I

2.7 ± 0.1

cde

phoP

Z2324ΔphoP

+++

0.15 ± 0.02

klm

I

2.45 ± 0.17

cdef

phoQ

Z2323ΔphoQ

+++

0.106 ± 0.01

lmno

I

2.25 ± 0.23

efghi

phoQP

Z2323–24 ΔphoQP

+++

0.18 ± 0.002

jk

I

2.25 ± 0.15

ijklm

pmrA

Z0089ΔpmrA

+++

0.05 ± 0.02

pqr

I

2.3 ± 0.19

hijkl

pmrB

Z0090ΔpmrB

+++

0.10 ± 0.01

lmno

I

2.40 ± 0.17

defgh

pmrBA

Z0089–90 ΔpmrBA

+++

0.07 ± 0.01

opq

I

2.3 ± 0.20

fghij

rcsB

Z3206ΔrcsB

---

0.002 ± 0.0005

r

I

2.51 ± 0.17

efghi

rcsC

Z3207ΔrcsC

---

0.4 ± 0.04

fg

I

2.45 ± 0.2

fghij

rcsD (yojN)

Z3205ΔrcsD

---

0.002 ± 0.0002

r

I

2.48 ± 0.28

efghi

rcsDB

Z3205–06 ΔrcsBD

---

0.003 ± 0.0001

r

I

2.43 ± 0.11

fghijk

rstA

Z2651ΔrstA

+++

0.359 ± 0.045

hgi

I

1.6 ± 0

st

rstB

Z2662ΔrstB

+++

0.1 ± 0.032

lmn

I

2.03 ± 0.06

opqr

spk1

Z1250Δspk1

+++

0.17 ± 0.04

kl

I

2.58 ± 0.14

cdef

ybjN

Z2074ΔybjN

+++

1.65 ± 0.12

a

I

2.05 ± 0.26

lmno

yciR

Z0955ΔyciR

+++

0.111 ± 0.0012

lmno

I

1.9 ± 0.08

pqr

yddV

Z1215ΔyddV

+++

0.043 ± 0.001

qr

C

2.9 ± 0.14

bc

yegE

Z3113ΔyegE

+++

0.22 ± 0.007

j

I

2.9 ± 0.14

bc

yehT

Z3996ΔyehU

+++

0.186 ± 0.026

k

I

2.5 ± 0.2

efghi

yehU

Z3995ΔyehT

+++

0.15 ± 0.04

klm

I

2.33 ± 0.21

fghij

yehUT

Z3995–96 ΔyehUT

+++

0.15 ± 0.004

klm

I

2.4 ± 0.17

fghij

yfhA

Z3498ΔyfhA

+++

0.07 ± 0.012

opq

I

2.3 ± 0.1

hijkl

yfhK

Z3495ΔyfhK

+++

0.06 ± 0.002

pq

I

2.33 ± 0.25

fghij

yfhKA

Z3495–98 ΔyfhKA

+++

0.062 ± 0.0005

pq

I

2.17 ± 0.12

lmno

yoaD

Z0355ΔyoaD

+++

0.22 ± 0.008

j

I

2.4 ± 0.37

ghijkl

  1. a ams operon encodes amylovoran biosynthesis. The entire operon (amsA-L; 15.8 kb) was deleted in the ams mutant [47]. flhD and fliA encode two regulators of flagella biosynthesis. These three mutants were used as negative controls for amylovoran biosynthesis and swarming motility, respectively.
  2. b Virulence assay was performed using immature pear fruits as described. +++: pathogenic as WT; ---: non-pathogenic.
  3. c Amylovoran production was measured 48 hr post inoculation. ND: not determined.
  4. d Distance of swarming motility was measured 48 hr post inoculation. Original circle was about 0.6 cm in diameter. I: irregular movement; C: circular. ND: not determined.
  5. e Values within the amylovoran production and distance of swarming motility columns followed by the same letter are not significantly different according to one way ANOVA and student-Newmans-Kleus test (P = 0.05).
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BMC Genomics

ISSN: 1471-2164

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