Figure 1

Quality of input RNA and targets synthesised from different amounts of input RNA. A, Bioanalyzer electrophoretic profile of the diluted Universal Human Reference RNA used as input for all amplifications. This profile corresponds to a classical and non-degraded human RNA with two fine characteristic peaks corresponding to 18S and 28S RNAs. 8.4 corresponds to the RNA Integrity Number (RIN) and reflects the high quality of this RNA B, electrophoretic profiles of cRNA obtained after one- and two-round Affymetrix amplification using 2 μg, and 100 ng of input RNA respectively or water as negative control. C, D, E, F, electrophoretic profiles of cRNA or cDNA obtained using Ambion (C), Arcturus (D), Epicentre (E) and Nugen (F) amplification systems from 250 pg and 500 pg of input RNA, or water as negative control.