Figure 1

The global transcriptomic profiles of adult female An. gambiae hemocytes. (a) Bar chart show the gene functional group distribution of all genes for which any transcription was detected in An. gambiae hemocytes, in at least 1 of the 5 experimental comparisons. (b) Bar chart showing the number and functional class of all the genes either enriched or under-represented in hemocytes relative to whole adult female mosquitoes (whole), or significantly differentially regulated in hemocytes either following challenge with heat-killed bacteria (E. coli and M. luteus) or infected with different stages of the rodent malaria parasite P. berghei (P. b. 24 hours and P. b. 19 days). Red and green arrows indicate, respectively, genes up- and down-regulated in hemocytes relative to either whole adult females (whole) or hemocytes from naïve control mosquitoes (E. coli, M. luteus, P. b. 24 hours and P. b. 19 days). (c) and (d) Proportional-area Venn diagrams illustrating the distribution of genes significantly differentially transcribed in hemocytes following either bacterial challenge (c) or different stages of infection with the malaria parasite P. berghei (d). Numbers in brackets outside circles indicate the total number of genes differentially regulated by each species, or stage, of pathogen. Numbers in brackets inside circles indicate the pooled total number of genes differentially regulated by either both stages of malaria parasite infection (c) or both species of bacteria (d). Numbers by red and green triangles indicate the number of genes up- and down-regulated, respectively, within each segment of the three segments formed by the two upper-most circles of each figure. IMM = immunity-and apoptosis-related; RED/STE = redox and oxidoreductive stress; PROT = proteolysis; CYT/STR = cytoskeletal and structural; TRP = transport; MET = metabolism; R/T/T = replication, transcription and translation; DIV = diverse; and UNK = unknown.