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Table 3 Q-PCR primer pairs used for the wheat control gene.

From: Comparative transcriptomics in the Triticeae

Gene

Forward primer

Reverse primer

Product size (bp)

ELF1

CAGATTGGCAACGGCTACG

CGGACAGCAAAACGACCAAG

227

GAPDH

TTCAACATCATTCCAAGCAGCA

CGTAACCCAAAATGCCCTTG

220

Cyclophilin

CAAGCCGCTGCACTACAAGG

AGGGGACGGTGCAGATGAA

227

Actin

GACAATGGAACCGGAATGGTC

GTGTGATGCCAGATTTTCTCCAT

236

  1. Primer sequence details for the control genes used in the Q-PCR verification of selected wheat expression profiles and the size of the corresponding RNA products.
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BMC Genomics

ISSN: 1471-2164

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