Figure 4
From: Gene expression patterns in heterozygous Plk4 murine embryonic fibroblasts
Assessment of levels of apoptosis and cell cycle arrest in heterozygous and wild-type Plk4 MEFs. A.) Levels of apoptosis after UV induced DNA damage in Plk4 heterozygous and wild-type MEFs. Apoptosis was analyzed prior to and after UV induced DNA damage (40 mJ/cm2) with a TUNEL assay. The results are presented as a percentage of apoptosis positive cells and are representative of three independent experiments. B.) Effect of UV induced DNA damage in Plk4 heterozygous and wild-type MEFs on progression through the cell cycle. Cell cycle profiles were analyzed prior to and after UV induced DNA damage (40 mJ/cm2) by flow cytometry after staining of the DNA with propidium iodide. The population of cells in G0/G1, S, G2/M and Sub G0 are displayed as percentage and are representative of three independent experiments.