Table 1 Oligonucleotide primer sequences for RT-PCR analysis.
From: Gene expression patterns in heterozygous Plk4 murine embryonic fibroblasts
Gene | Primers | Size (bp) |
|---|---|---|
GAPDH | Forward 5'GCTGAGTATGTCGTGGAGTCT-3' Reverse 5'-CAGAGCTGAACGGGAAGCTC-3' | 410 |
Plk4 | Forward 5'-AGGGAAGCTAGGCACTTCATG-3' Reverse 5'-GGAAGACCACCTTTTGAC-3' | 310 |
Sap30 | Forward 5'-CCAGAAGCTCTACGAGCGGAA-3' Reverse 5'TGGTCTGAAGACTCCTACTATGAG-3' | 190 |
Prohibitin | Forward 5'-CGTATCTACACCAGCATTGGC-3' Reverse 5'-TGTGGTGGAAAAGGCTGAGC-3' | 301 |
Wisp1 | Forward 5'-GCCTAATCACAGATGGCTGTG-3; Reverse 5'-CAATAGGAGTGTGTGCACAGGTG-3' | 150 |