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Table 3 Top 10 most frequently detected unigenes in 454 cDNA libraries of Arabidopsis, Eschscholzia, and Persea.

From: Comparison of next generation sequencing technologies for transcriptome characterization

Library

Contig

Len

Reads

Cov

AGI

Len

Evalue

Annotation

Ath-rand

08061

357

586

34.8

AT1G67090

1025

0.0

RuBisCO small subunit 1A (RBCS-1A) (ATS1A)

Ath-rand

00035

1326

541

96.8

AT1G54040

1370

0.0

TASTY, ESP (EPITHIOSPECIFIER PROTEIN)

Ath-rand

08724

1653

391

90.0

AT5G26000

1836

0.0

TGG1 (THIOGLUCOSIDE GLUCOHYDROLASE1)

Ath-rand

08295

1175

278

94.6

AT2G42840

1242

0.0

PDF1 (PROTODERMAL FACTOR 1)

Ath-rand

08670

310

258

31.5

AT5G38410

984

4e-175

RuBisCO small subunit 3B (RBCS-3B) (ATS3B)

Ath-rand

00011

240

229

23.4

AT1G67090

1025

9e-43

RuBisCO small subunit 1A (RBCS-1A) (ATS1A)

Ath-rand

00660

640

219

76.9

AT2G21660

832

2e-157

ATGRP7 (Cold, Circadian Rhythm, RNA Binding 2)

Ath-rand

07960

927

215

52.6

AT5G60390

1764

0.0

elongation factor 1-alpha/EF-1-alpha

Ath-rand

04760

1157

206

82.3

AT3G12145

1406

0.0

FLR1 (FLOR1); enzyme inhibitor

Ath-rand

08550

373

190

100

ATCG00220

105

3e-53

PSBM, PSII low MW protein

Eca-oligo

19682

387

850

83.2

AT5G39170

465

2e-7

Unknown protein

Eca-oligo

19707

2089

784

100

AT1G70370

1878

0

BURP domain-containing protein/polygalacturonase

Eca-oligo

18128

151

707

10.0

AT3G47550

1505

0.02

C3HC4-type RING finger family protein

Eca-oligo

19695

308

678

100

AT5G52160

288

1e-15

protease inhibitor/seed storage/lipid transfer protein

Eca-oligo

19793

940

608

100

AT2G36830

753

6e-102

GAMMA-TIP (Tonoplast intrinsic protein gamma)

Eca-oligo

18734

849

485

100

AT3G16640

504

7e-52

TCTP (Translationally Controlled Tumor Protein)

Eca-oligo

00048

2823

450

80.0

AT5G35750

3528

0

AHK2 (Arabidopsis Histidine Kinsase 2)

Eca-oligo

18697

144

450

24.7

AT4G06746

584

0.31

RAP2.9 (related to AP2 9); transcription factor

Eca-oligo

19623

2638

421

81.4

AT2G01830

3240

0

WOL (CYTOKININ RESPONSE 1)

Eca-oligo

19622

120

415

6.4

AT1G23800

1866

1

ALDH2B7 (Aldehyde dehydrogenase 2B7)

Eca-rand

15341

109

4296

6.9

AT4G03930

1575

0.23

Pectinesterase

Eca-rand

15345

162

4274

12.0

AT3G59430

1353

0.33

Unknown protein

Eca-rand

15162

315

852

19.7

AT5G26670

1596

0.18

Pectinacetylesterase, putative

Eca-rand

15258

606

726

53.3

AT3G12340

1137

0.1

FK506 binding/peptidyl-prolyl cis-trans isomerase

Eca-rand

14312

182

682

56.2

ATMG00030

324

2e-77

ORF107A

Eca-rand

15290

2020

674

100

AT1G70370

1878

0

BURP domain-containing protein/polygalacturonase

Eca-rand

15208

1052

514

100

AT2G36830

753

7e-102

GAMMA-TIP (Tonoplast intrinsic protein gamma)

Eca-rand

14424

2660

480

75.4

AT5G34750

3528

2e-162

AHK2 (ARABIDOPSIS HISTIDINE KINASE 2)

Eca-rand

15320

1146

437

48.3

AT5G02500

2373

0

HSC70-1 (heat shock cognate 70 kDa protein 1)

Eca-rand

15269

304

417

5.8

AT2G47410

5221

0.2

Nucleotide binding

Pam-norm

15603

133

37

9.8

AT1G59830

1357

0.005

PP2A-1 (protein phosphatase 2A-2)

Pam-norm

18074

139

32

10.4

AT1G14270

1343

0.3

CAAX amino terminal protease family protein

Pam-norm

8473

176

27

10.4

AT4G17890

1688

0.1

AGD8, UBP20 (Ubiquitin-specific Protease 20)

Pam-norm

14132

213

26

7.3

AT2G40820

2907

1.9

Proline-rich family protein

Pam-norm

15140

237

26

48.5

AT2G41430

489

2e-13

ERD15 (Early Responsive To Dehydration 15)

Pam-norm

4395

144

25

6.4

AT1G45545

2259

0.08

Similar to unknown protein

Pam-norm

15762

102

24

3.5

AT1G01950

2901

0.2

Armadillo/beta-catenin repeat family protein

Pam-norm

10833

112

20

6.4

AT3G03640

1747

0.001

GLUC (Beta-glucosidase homolog)

Pam-norm

18760

253

19

59.0

AT4G14270

429

2e-04

Protein containing PAM2 motif

Pam-norm

18306

208

18

48.5

AT4G14270

429

8e-05

Protein containing PAM2 motif

  1. Unigenes from each library, Arabidopsis flower bud random-primed (Ath-rand), Eschscholzia flower bud oligo-dT (Eca-oligo) and random-primed (Eca-rand), and Persea americana normalized flower bud (Pam-norm), were mapped to the annotated TAIR cDNA and protein datasets using BLASTx (e-5 cutoff). Column headers are contig name (Contig), contig length (Len), number of reads per contig (Reads), percent coverage (Cov), Arabidopsis best hit gene identifier (AGI), annotation (Annotation), and E-value. Ribosomal RNA and contaminants such as putative endophytes removed from this list. Refer to Additional file 1 for detailed BLAST results.