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Figure 2 | BMC Genomics

Figure 2

From: GATA3 is a master regulator of the transcriptional response to low-dose ionizing radiation in human keratinocytes

Figure 2

Silencing GATA3 expression in human keratinocytes transduced with the various GATA3 shRNA vectors. (A) Quantitative RT-PCR analysis of GATA3 transcripts at various time points after infection of human keratinocytes with the various shRNA vectors. The repression fold of the GATA3 transcript versus cells infected with the shSCR vector is indicated. (B) Analysis of GATA3 and beta-actin (ACT) protein levels by immunoblotting in human keratinocytes infected either with the sh299 (shGATA3) or the shSCR vector at 48 or 72 hours after infection.

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