Table 2 Characterization of cDNA citrus libraries.
Library | Clones | High quality ESTs | Mean EST length | Singletons | Contigs | Contigs with 2-3 reads | Unigenes | Redundancy (%) | Library- specific unigenes | Novelty (%) | Full- length clones | ESTs with orthologue | Fullness | Full- length library- specific unigenes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Full-length enriched libraries | ||||||||||||||
AbioticL1 | 960 | 831 (87) | 673 | 125 | 498 | 481 (97) | 623 | 25 | 139 | 22 | 613 | 794 (96) | 77 | 46 |
AbioticR1 | 960 | 903 (94) | 667 | 157 | 531 | 514 (97) | 688 | 24 | 168 | 24 | 698 | 853 (94) | 82 | 49 |
BiotPhyR1 | 960 | 919 (96) | 681 | 364 | 401 | 389 (97) | 765 | 17 | 407 | 53 | 515 | 752 (82) | 68 | 31 |
CTVMacrop1 | 480 | 447 (93) | 651 | 133 | 214 | 205 (96) | 347 | 22 | 147 | 42 | 350 | 412 (92) | 85 | 58 |
CTVClemen1 | 480 | 453 (94) | 682 | 62 | 327 | 324 (99) | 389 | 14 | 63 | 16 | 329 | 426 (94) | 77 | 48 |
CEVdCidro1 | 480 | 465 (97) | 693 | 111 | 220 | 214 (97) | 331 | 29 | 152 | 46 | 330 | 446 (96) | 74 | 59 |
HSVdMacro1 | 480 | 406 (85) | 654 | 116 | 209 | 201 (96) | 325 | 20 | 128 | 39 | 281 | 373 (92) | 75 | 62 |
DevOvary1 | 384 | 296 (77) | 637 | 27 | 192 | 185 (96) | 219 | 26 | 29 | 13 | 251 | 291 (98) | 86 | 69 |
RVDevelop1 | 960 | 759 (79) | 669 | 60 | 451 | 433 (96) | 511 | 33 | 65 | 13 | 612 | 737 (97) | 83 | 46 |
PostharvC1 | 960 | 917 (96) | 697 | 134 | 582 | 565 (97) | 716 | 22 | 137 | 19 | 706 | 886 (97) | 80 | 44 |
PostharvP1 | 1056 | 1008 (95) | 684 | 161 | 640 | 624 (98) | 801 | 21 | 169 | 21 | 662 | 966 (96) | 69 | 44 |
TOTAL | 8160 | 7404 (91) | Â | 1450 | 2914 | Â | 4364 | Â | 1604 | 37 | 5347 | 6936 (94) | 78 | 556 |
Normalized and full-length enriched libraries | ||||||||||||||
RVDevelopN | 960 | 823 (86) | 640 | 182 | 543 | 540 (99) | 725 | 12 | 192 | 26 | 560 | 755 (92) | 74 | 44 |
StrClemenN | 960 | 866 (90) | 658 | 186 | 567 | 563 (99) | 753 | 13 | 203 | 27 | 544 | 810 (94) | 67 | 45 |
StrCleopN | 960 | 914 (95) | 693 | 246 | 594 | 594 (100) | 840 | 8 | 254 | 30 | 655 | 866 (95) | 76 | 51 |
PostharveN | 928 | 891 (96) | 684 | 176 | 561 | 552 (98) | 737 | 17 | 185 | 25 | 636 | 840 (94) | 76 | 41 |
TOTAL | 3808 | 3494 (92) | Â | 790 | 1882 | Â | 2672 | Â | 834 | 31 | 2395 | 3271 (94) | 73 | 181 |
BIG TOTAL | 11968 | 10898 (91) | 673 | 2240 | 3902 | Â | 6142 | Â | 2438 | 40 | 7742 | 10207 (94) | 76 | 737 |