Figure 5

Androgen regulation of genes in the in vivo Hollow Fibre model of prostate cancer. Levels of transcripts in LNCaP cells from the Hollow Fibre model were analyzed by qRT-PCR. Cx, castrated mice, 10 days post castration, n = 12; Pre-Cx, pre-castration, day 0 of castration, n = 15. Exception: LRIG1 gene expression in Cx samples was represented by 11 mice. Fold-change was calculated by normalizing the mean normalized expression (MNE) of transcripts in the Pre-Cx sample to the castrate sample. In doing this, the Cx sample fold-change became one and standard deviation (SD) zero. Error bars represent ± SD. [*] Asterisks indicate the significant differential gene expression with respect to Cx according to the Two-Sample Student's T-test (p ≤ 0.05) for unequal variance.