Identification and analysis of serpin-family genes by homology and synteny across the 12 sequenced Drosophilid genomes

Table 2 Sequence conservation in serpin core and RCL between D. melanogaster and D. grimshawi

Serpin	P1/P1' mel	P1/P1' gri	Alignment Score % Serpin core	Alignment Score % RCL
Spn27A	K/F	K/F	70	87
Spn28Dc	S/G	S/G	45	76
Spn31A	-	-	67	80
Spn42Da	A/S	A/S	66	69
Spn42Db	K/G	R/S	46	49
Spn42Dc	M/M	M/S	52	75
Spn43Aa	M/S	M/S	80	95
Spn43Ab	-	-	77	90
Spn43Ac	L/S	L/S	41	72
Spn43Ad	-	-	53	46
Spn47C	-	-	41	44
Spn55B	R/M	R/M	76	94
Spn77Ba	K/A	K/S	58	72
Spn85F	-	-	68	96
Spn88Ea	S/A	S/A	55	84
Spn100A	-	-	62	67

The putative proteinase cleavage sites (P1/P1') of inhibitory serpins rarely change between the D. melanogaster and D. grimshawi orthologues and the RCL sequence is more strongly conserved than the serpin core. The RCL of non-inhibitory serpin-folds tends to be weakly conserved, with three exceptions, Spn31A, Spn43Ab and Spn85F. The putative RCL of the serpin-related fold, Spn100A, is conserved slightly more strongly than the remainder of the protein.

ISSN: 1471-2164