Figure 5

Changes in number and acidity of goblet cells in E isolate-infected C57BL/6 mice compared to S isolate-infected C57BL/6 mice. C57BL/6 mice were infected with either the E or S isolates of Trichuris muris. At each time point mice infected with either the E or S isolate were sacrificed and goblet cells were detected in proximal colon tissue sections via immunohistochemistry. Data are presented as mean number of goblet cells per 20 ccu (A), mean number of neutral (stain pink following alcian blue and periodic acid treatment) goblet cells per 20 ccu (B) and percentage of neutral goblet cells (C). Examples of goblet cell staining (×200 and ×400 magnification) in E isolate-infected or S isolate-infected mice at d7 p.i (D). *; p < 0.05 and **; p < 0.01 for comparison between the E isolate and S isolate-infected mice (Mann Whitney U test). Experiments were performed at least twice and data shown is a representative experiment. Values represent the mean ± SD for five mice per group.