Figure 2

Specific DNA binding of HaloTag-CREB in vivo. HaloCHIP experiments were performed in triplicates, on HeLa cells transiently expressing HaloTag-CREB or untransfected as a control. Resulting DNA from both the HaloTag-CREB and Untransfected control sample was amplified and analyzed using Plexor quantitative PCR. Total amounts of DNA for both samples were calculated for three promoters which CREB is known to bind [28], Fos, Jun, and p27, as well as three control sequences, C1, C2, and C3, which do not contain CRE consensus binding sites. Depicted in dark blue is the fold enrichment of each CREB-specific promoter over the average amount of the three control promoters for the HaloTag-CREB HaloCHIP experimental sample. In light blue is the identical calculation for the Untransfected HaloCHIP control sample.