Table 3 GUS expression in the F2 generation of gene trap lines
From: An Ac/Ds-mediated gene trap system for functional genomics in barley
GTDs B line | Gene trap line | No. plants analysed | No. plants with GUS | Organ with GUS activity (no. explants) |
|---|---|---|---|---|
2a/d | GT32 | 6 | 2 | grain (1), seedling (1) |
GT73 | 4 | 1 | grain (1) | |
23 | GT33 | 4 | - | - |
GT29 | 4(1c) | 2 | pollinated floret (1), seedling (1) | |
GT39 | 1 | - | - | |
GT41 | 5 | 3 | pollinated floret (13), grain (4), seedling (1) | |
31B | GT49 | 4 | 2 | pollinated floret (2), seedling (1) |
GT54 | 5 | 5 | immature (7)/pollinated (1) floret, grain (10), seedling (15) | |
6a/c | GT70 | 4 | 1 | pollinated floret (1), node (1) |
GT67 | 6 | 4 | node (4), leaf (1) | |
GT4 | 7(1a) | - | - | |
31A | GT38 | 6 | 1 | seedling (1) |
GT52 | 5(1d/1e) | 4 | node (1), grain (10) | |
16 | GT19 | 4b | 1 | grain (1) |
GT37 | 8 | 2 | grain (4), seedling (1) | |
GT40 | 4 | - | - | |
14A | GT13 | 4(2b/2c) | 2 | grain (1), seedling (7) |
GT22 | 5(4b/1c) | 4 | grain (2), seedling (17) | |
11 | GT82 | 3 | 3 | immature floret (1), node (3), leaf (1) |
GT66 | 3(f) | 2 | pollinated floret (3), grain (1) | |
GT80 | 4 | 4 | immature floret (3), pollinated floret (2), node (4), leaf (1), grain (9), seedling (2) | |
10 | GT9 | 7(7b) | 2 | grain (2), seedling (2) |
GT2 | 7(6b/1c) | 5 | grain (2), seedling (4) | |
GT6 | 7(5b/2c) | 4 | grain (6), seedling (3) | |
14B | GT23 | 4(2a/1d) | 1 | seedling (1) |
GT15 | 8(1b/3c) | 7 | pollinated floret (9), grain (12), seedling (17) | |
26 | GT35 | 4 | 4 | immature floret (41), pollinated floret (32) |
GT63 | 8 | 7 | immature floret (3), grain (23), seedling (2) | |
Total | 141 | 72 |