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Table 4 Gene trap F2 plants with GUS expression in at least 50% of the explants

From: An Ac/Ds-mediated gene trap system for functional genomics in barley

F2 plant

No. of explants analysed

No. of explants with GUS

Explant type

GT41/3

16

13

pollinated florets

GT54/5

8

5

seedlings

GT54/10

8

5

seedlings

GT13/8

8

5

seedlings

GT22/3

8

6

seedlings

GT22/5

8

8

seedlings

GT15/2

8

5

seedlings

GT15/4

8

4

seedlings

GT15/9

9

9

pollinated florets

GT35/1

8

8

pollinated florets

GT35/3

8

8

pollinated florets

GT35/7

8

8

pollinated florets

GT35/8

8

8

pollinated florets

GT63/4

16

9

grains