Figure 1
From: Annotation and expression of carboxylesterases in the silkworm, Bombyx mori
Neighbor-joining tree of amino acid sequences from B. mori , Ap. mellifera , and D. melanogaster , and some known COEs in other Lepidopteran insects. Bootstrap values > 70% are shown. The catalytic triad was predicted by blastp searching NCBI conserved domain database (CDD) http://www.ncbi.nlm.nih.gov/Structure/cdd/cdd.shtml using COE amino acid sequences as queries. Thus, the catalytic residues (S200, E327 and H440) and GXSXG consensus sequence around the active site serine were presented. Short dash: absence of the catalytic residue. Red: B. mori; Green: Ap. mellifera; Blue: D. melanogaster; Black: other Lepidoptera. Snon (Sesamia nonagrioides), Apol (Antheraea polyphemus), Slit (Spodoptera littoralis), Mbra (Mamestra brassicae), Sn (Sesamia nonagrioides), Cf (Choristoneura fumiferana), Hv (Heliothis virescens), Of (Omphisa fuscidentalis), Ms (Manduca sexta), Tm (Tenebrio molitor), Ph (Psacothea hilaris), Ga (Gryllus assimilis).