Genes and gene expression modules associated with caloric restriction and aging in the laboratory mouse

BMC Genomics

Table 2 CR-regulated co-expression modules

Modules	M (P-Value)	Minimal \|r\|
{Fmo2, 1200016E24Rik}	10.7 (0.062)	0.703
{Hist2h3c1, Hist2h3c2}	10.5 (0.136)	0.817
{Per1, Per2}	10.1 (0.393)	0.597
{Hist2h3c1, Hist2h3c2, Hist1h2bc}	10.2 (0.004)	0.700
{Per1, Per2, Zbtb16}	10.0 (0.008)	0.596
{Epm2aip1, Casc4, 2810455D13Rik}	9.73 (0.033)	0.568
{Hist2h3c1, Hist2h3c2, Hist1h2bc, Hist2h2aa1, Hist1h1c}	9.79 (< 0.001)	0.595
{Per1, Per2, Zbtb16, Mm.34106, 1439537_at}	9.29 (< 0.001)	0.564
{Alkbh8, Zfp148, Rc3h2, Zdhhc21, Taok1}	8.91 (0.006)	0.494

The table lists the most significant CR-regulated co-expression modules containing 2, 3, or 5 genes. The M statistic is an indicator of responsiveness to CR, with larger values suggesting stronger effects of CR on the expression of genes contained in a given module (see Methods). P-values associated with M statistics were generated by simulation, and reflect the likelihood of obtaining an equal or larger M value by randomly selecting genes to form a module of the same size (see Methods). The final column provides an indication of the degree to which module members are co-expressed with one another. This value is an element of the [0, 1] interval, and is obtained by calculating all possible pair-wise co-expression relationships among members of the module (all possible values of r), and identifying the weakest co-expression relationship between any two module members (i.e., the minimal value of |r|). Smaller minimal |r| values indicate more "diffuse" co-expression modules with weaker co-expression relationships, and larger minimal |r| values indicate "tighter" co-expression modules with stronger co-expression relationships. Additional File 6 provides a more detailed description of these and other co-expression modules that emerged as significantly associated with CR.

ISSN: 1471-2164