Figure 2

Populus produces phased siRNAs. A Putative non-conserved trans-acting siRNA locus (LG_X:19646453..19647187). Hypothetical and sequenced small RNAs are shown in orange and purple/moss-green respectively. Regions detected by RepPop are shown as black boxes, predicted miRNA target sites are shown in dark blue and the predicted TAS locus is shown as a purple box. Sequenced siRNA are shown with a black border, with sequences in phase with the TAS locus with a purple border. siRNAs are shaded by raw count value: light-blue to dark blue in the range 1-100 and orange > 100. B Sequence counts for 21 nt siRNAs in the region in A. Inset: Phasing register expression plot showing clear phasing and small RNA size distribution showing a majority of 21-mers from the region. C Sequence counts for 21 nt siRNAs within (LG_VI:551356..551607) including phase distributions count and small RNA size distribution. D Sequence counts for 21 nt siRNAs within the Populus ortholog of Arabidopsis thaliana TAS3a (LG_X:14146030..14146540), including phase distributions count and small RNA size distribution.