Figure 1From: Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesisComparison of growth rate and internal pH of C. glutamicum exposed to different external pH values. Growth experiments were performed in selected buffer systems in shaken microtiter plates (pH 4-5.5 black circles: Homopipes, pH 5.5-6.5 white triangles down: Mes, pH 6.5-7.75 black squares: Mops, pH 7.5-8.75 white diamonds: Hepps, pH 8.75-9.75 black triangles: Ches, pH 9.75-11 white hexagons: Caps). Determination of the cytoplasmic pH (white triangles top) was performed in a separate experiment by measuring the distribution of the radioactive probes benzoic acid (pH 4 to 7.5) and methylammonium (pH 7.5-11, see Methods section).Back to article page