Potential impact of stress activated retrotransposons on genome evolution in a marine diatom
© Maumus et al; licensee BioMed Central Ltd. 2009
Received: 26 August 2009
Accepted: 22 December 2009
Published: 22 December 2009
Transposable elements (TEs) are mobile DNA sequences present in the genomes of most organisms. They have been extensively studied in animals, fungi, and plants, and have been shown to have important functions in genome dynamics and species evolution. Recent genomic data can now enlarge the identification and study of TEs to other branches of the eukaryotic tree of life. Diatoms, which belong to the heterokont group, are unicellular eukaryotic algae responsible for around 40% of marine primary productivity. The genomes of a centric diatom, Thalassiosira pseudonana, and a pennate diatom, Phaeodactylum tricornutum, that likely diverged around 90 Mya, have recently become available.
In the present work, we establish that LTR retrotransposons (LTR-RTs) are the most abundant TEs inhabiting these genomes, with a much higher presence in the P. tricornutum genome. We show that the LTR-RTs found in diatoms form two new phylogenetic lineages that appear to be diatom specific and are also found in environmental samples taken from different oceans. Comparative expression analysis in P. tricornutum cells cultured under 16 different conditions demonstrate high levels of transcriptional activity of LTR retrotransposons in response to nitrate limitation and upon exposure to diatom-derived reactive aldehydes, which are known to induce stress responses and cell death. Regulatory aspects of P. tricornutum retrotransposon transcription also include the occurrence of nitrate limitation sensitive cis-regulatory components within LTR elements and cytosine methylation dynamics. Differential insertion patterns in different P. tricornutum accessions isolated from around the world infer the role of LTR-RTs in generating intraspecific genetic variability.
Based on these findings we propose that LTR-RTs may have been important for promoting genome rearrangements in diatoms.
Transposable elements (TEs) are mobile genetic sequences found within the genomes of most organisms. Sequences derived from TEs represent a genomic fraction of 3% in baker's yeast , ~20% in fruit fly [2–4], 45% in human [5, 6] and over 80% in maize [7, 8]. They are thought to be important contributors to genome evolution by inserting into genes or genetic regulatory elements, thereby disrupting gene function, altering levels of gene expression, triggering chromosomal rearrangements, and adding to or subtracting from the physical size of a host genome . TEs are classified into two groups based on their mode of transposition: retrotransposons or Class 1 TEs which replicate through reverse transcription of an mRNA intermediate, and DNA transposons or Class 2 TEs that use a "cut and paste" mechanism.
A pervasive group of retrotransposons are those flanked by long terminal repeats (LTRs), also typical of retroviruses to which they are related. The LTR direct sequence repeats flank the internal region that encodes both structural and enzymatic proteins with homology to the GAG and POL proteins of retroviruses. The gag gene encodes structural proteins that form the virus-like particle (VLP), inside which reverse transcription takes place. The pol gene encodes several enzymatic functions, including a protease (PR) that cleaves the POL polyprotein, a reverse transcriptase (RT) that copies the retrotransposon RNA into cDNA, a ribonuclease H domain (RH), and an integrase (IN) that integrates the cDNA into the genome. Two main groups of LTR retrotransposons (LTR-RTs) are found throughout eukaryotes, and are distinguished by the organization of their pol genes and similarities among their encoded RT proteins . These groups are referred to as Ty1/copia elements (Pseudoviridae) and Ty3/gypsy elements (Metaviridae), which respectively display a PR, IN, RT, RH and PR, RT, RH, IN gene organization.
The unicellular chlorophyll c-containing algal class Bacillariophyceae (diatoms) is among the most successful and diversified groups of photosynthetic eukaryotes, with possibly over 100,000 extant species  widespread in all kinds of humid and open water environments. The contribution of diatom photosynthesis to marine primary productivity has been estimated to be around 40% [12, 13]. Diatoms have a peculiar genetic makeup because they are likely to have emerged following a secondary endosymbiotic process between a photosynthetic eukaryote, most probably red algal-like, and a heterotrophic eukaryote . They are traditionally divided into two orders: the centric diatoms which are radially symmetrical and are thought to have arisen around 180 Million years ago (Mya), followed by the pennate diatoms around 90 Mya which are bilaterally symmetrical. Genome sequences of the centric diatom Thalassiosira pseudonana and the pennate diatom Phaeodactylum tricornutum have recently become available [15, 16]. Because diatoms are single celled organisms that typically reproduce mitotically, the activity of LTR-RTs might have particularly profound effects on genome evolution since any non-lethal retroelement insertion will be transmitted to subsequent generations.
In an analysis of the T. pseudonana genome, Armbrust and collaborators identified several TEs . In the current work, we have identified additional TEs in both diatom genomes and we show that LTR-RTs are the most abundant elements, particularly in P. tricornutum where they have amplified enormously. Phylogenetic analysis of the RT domain shows that diatom Ty1/copia-like elements belong to different lineages, and that two of them are diatom specific. Examination of the CAMERA metagenomic database reveals that these elements are also widespread in different oceans. The potential ecological relevance of these elements for driving genome and population evolution and heterogeneity has been assessed by examining their expression in response to stress as well as their distribution in P. tricornutum accessions collected from different locations worldwide. We also examine whether or not cis-regulatory elements within LTR sequences contain sufficient information for driving retrotransposon transcription in response to nitrate deprevation and if alterations in cytosine methylation play a role in retrotransposon expression.
Expansion of LTR Retrotransposons in the P. tricornutum genome
To analyse the contribution of TEs to diatom genomes we used the diatom TE DNA sequences to run the RepeatMasker program  on both genomes. In total, we found that TEs contribute 1,665 kb (6.4%) of the P. tricornutum genome and 590 kb (1.9%) of the T. pseudonana genome. Of these, LTR-RTs are the most abundant in both genomes and constitute 90% and 58% of the P. tricornutum and T. pseudonana TE complement, respectively (Figure 1A and 1B). Harbinger elements also appear to represent a significant proportion in T. pseudonana. In total, the RepeatMasker output indicated that sequences deriving from LTR-RTs make up 5.8% of the P. tricornutum genome  and 1.1% of the T. pseudonana genome  (Figure 1C). It thus appears that Ty1/copia-like LTR-RTs have significantly expanded in the P. tricornutum genome.
Classification of LTR retrotransposon sequences
To further investigate the diatom LTR-RT elements, we manually screened the P. tricornutum and T. pseudonana nuclear genomes for the presence of putatively autonomous LTR-RTs (see Materials and Methods), and found a total of 42 and 13 putative active elements in the final unmasked assemblies of the P. tricornutum and T. pseudonana nuclear genomes, respectively. Most of these have greater than 95% identical LTR pairs and display only one or no stop codon/frameshifts between the gag and pol genes (Additional file 1 and Materials and Methods). All the selected sequences from P. tricornutum and 11 from T. pseudonana belonged to the Ty1/copia class with pol domains ordered as expected (PR, IN, RT, RH), and the two remaining sequences from T. pseudonana belonged to the Ty3/gypsy class with pol domains also ordered in a typical fashion (PR, RT, RH, IN).
The 53 Ty1/copia-like elements identified in the P. tricornutum and T. pseudonana genomes were classified on the basis of RT domain sequence similarity (see Materials and Methods). Seven groups of Ty1/copia-like retroelements were identified and denoted CoDi1 to CoDi7 (Ty1/ Co pia-like elements from Di atoms) (Additional file 1, Figure 1). While the CoDi1 to CoDi5 groups are quite homogeneous, the CoDi6 group consists of a set of diverse elements. The CoDi7 group is composed of a single element from P. tricornutum (PtC47). The CoDi1-2-3-7 groups are specific to P. tricornutum whereas the CoDi4-5-6 groups are composed of elements found in both diatom genomes. It appears that the CoDi2 and CoDi4 groups are major components of LTR-RT expansion in the P. tricornutum genome (Figure 1D).
Expression of LTR retrotransposons in P. tricornutum and T. pseudonana
DD2 2 hrs
DD2 6 hrs
DD2 30 hrs
DD2 4 days
24 hrs (N limitation)
2 weeks (N limitation)
Regulation of Blackbeard
Cytosine methylation is commonly found in the DNA sequences of transposable elements (at least in genomes in which methylation occurs) and is thought to be involved in the heterochromatin formation and maintenance that controls TE mobility. TE mobilization has been shown to be associated with DNA hypomethylation [22–24], and hypomethylation has also been found to accompany active transposition in response to stress [25, 26]. We therefore assessed whether the Blackbeard element was methylated using McrPCR. In this method, DNA is digested with McrBC which cleaves DNA containing methylcytosine. Consequently, PCR using McrBC-digested DNA as template leads to a decrease of amplification at methylated (cut) loci with respect to untreated DNA. We first observed that all LTR-RTs tested were methylated in the P. tricornutum genome under normal growth conditions (data not shown), demonstrating that DNA methylation does occur in this diatom. We then compared McrPCR amplification levels using DNA extracted from P. tricornutum cells grown in normal and nitrate limiting conditions. Figures 5B and 5C show that the induction of Blackbeard in response to nitrate limitation was accompanied by a decrease in cytosine methylation, suggesting that chromatin remodeling occurs at the Blackbeard locus in response to nitrate limitation. Preliminary results from bisulfite sequencing indicate that methylation at the Blackbeard locus occurs in a CpG context (data not shown).
Insertion polymorphism between P. tricornutum accessions
Two distinct haplotypes at loci containing TEs
TE-mediated recombination in the P. tricornutum genome
To shed light on the potential impact of LTR-RTs on genome dynamics, we analyzed some signatures of intra- or inter-element recombination in the P. tricornutum and T. pseudonana genomes . Unequal intrastrand homologous recombination between LTRs of different elements belonging to the same family is a typical example and can result in a net loss of the DNA in between the elements involved. Five examples of this were found in our study of the P. tricornutum genome, all of which resulted in clearly recognizable recombinant products in which apparently intact elements with more than 99% identical LTRs lacked the target-site duplication (TSD) (see Additional file 1) and were therefore expected to be the product of homologous recombination between two family members. On the other hand, we found no example of this kind in the T. pseudonana genome.
Furthermore, these two genomic regions contain a group of 5 orthologs of an unknown gene family (see Figure 8 and Additional file 4). The segment containing the two copies located on chromosome 31 and their intergenic region is located less than 1 kb downstream of the CoDi5.3-like element and is highly similar (>97% identity) to the segment containing two of the copies located on chromosome 11 and their intergenic region. The Pt2_50888 gene in fact appears to be the product of recombination between two distinct orthologs as its beginning and downstream region is similar to the Pt2_46949 locus and its end and upstream region appears most similar to the Pt2_46950 and Pt2_50889 loci (Additional file 4). A >7 kb region between the Pt2_46950 locus and the CoDi5.3 segment is also duplicated elsewhere in the genome. These loci therefore provide compelling evidence for TE-mediated recombination events in the P. tricornutum genome.
A high diversity of RT domains from micro-planktonic organisms
In this work, we have identified seven groups of Ty1/copia-like LTR-retrotransposons in diatom genomes. Four groups (CoDi1-2-3 and CoDi7) were found only in the P. tricornutum genome whereas elements belonging to the CoDi4-5-6 groups were detected in both diatom genomes. The presence of both classes suggests either that they were present in the diatom common ancestor and that the CoDi1-3 groups became extinct in the lineage leading to the centric species T. pseudonana, or that representatives of each group have been separately introduced horizontally in pennate and centric diatoms. The topology of the tree presented in Figure 2 shows that CoDi3 and CoDi4 are bootstrap-supported sister groups that share a common ancestor after the separation from CoDi1 and CoDi2. This, together with the fact that we could not detect traces of diverged remnant copies from the CoDi1-3 groups in the T. pseudonana genome by BLAST searches (data not shown) favors the horizontal transfer hypothesis to explain the presence of CoDi4 elements in the T. pseudonana genome.
Ty3/gypsy-like elements were found in the T. pseudonana genome but not in the P. tricornutum genome. We also identified RT sequences corresponding to Ty3/gypsy-like elements from the pennate diatoms P. multiseries and P. multistriata which clearly cluster with the GyDi elements (Figure 3). Although the number of diatom species for which data is available is low, this suggests that Ty3/gypsy-like elements were likely present in the diatom common ancestor, and that these elements have been lost in P. tricornutum.
Figure 10 shows the retrotransposon sequences found in the CAMERA dataset. Although the vast majority of the sequences derived from these environmental genomic surveys are of bacterial and archaeal origin , the authors counted 69 18S rRNA sequences in the analysis of the Sargasso Sea data  and 98 in the GOS sequence collection (Doug Rusch, personal communication). Thus, some small eukaryotes were also present in these datasets. The observed higher abundance of RT domains in the fractions containing the larger cells is consistent with higher relative eukaryote/prokaryote abundance in these samples. The RT sequences studied display a huge diversity including some clustering in the CoDiI and CoDiII lineages, which likely testifies for the presence of diatoms in the samples. The other RT sequences may reflect the diversity of LTR retrotransposons populating the genomes of diverse tiny marine eukaryotes such as green, red, or brown algae, dinoflagellates, haptophytes, or euglenoids. For example, the abundance of RAS-clustering sequences in the Sargasso Sea fractions may be indicative of the presence of red algae, although analysis of these eukaryotic fractions did not reveal a particular abundance of red algae . It will therefore be important to determine which eukaryotic branch or branches the RAS-like sequences collected come from. In addition to the CoDiI, CodiII, and RAS sequences, other discrete clusters shown in Figure 10 are exclusively composed of RT sequences from the CAMERA database and are likely to represent RT domains from organisms for which we have little or no genomic knowledge.
The mutagenic potential of LTR retrotransposons  and the effects of their accumulation  and recombination  together suggest that active retrotransposons may be major contributors to genome diversification. Accumulated data indicates that retrotransposons in plants , animals and fungi respond to various forms of stress. It has also been shown in natural wild barley populations living on each side of a canyon that LTR retrotransposon dynamics contribute to genome diversity in response to sharp microclimatic divergence . LTR-RTs are hence thought to play a key role in long term adaptation of natural populations exposed to stress by generating genetic diversity within populations. Evidence presented here suggests that this may also be the case in diatoms. For example, Blackbeard is one of the most highly expressed genes in the EST library derived from P. tricornutum cells grown under nitrate starvation and Surcouf is highly expressed in response to DD treatment (Table 1, Figure 4). If these expression levels correlate with completion of the retrotransposition cycle, which ends with de novo insertions, then nitrate starvation, DD exposure, and perhaps other environmental stressors could lead to an increase in genetic diversity in P. tricornutum. LTR-RTs may therefore be major drivers of genetic diversity in P. tricornutum populations. Although we have not been able to observe de novo insertion of Bkb or Scf elements following stress, this claim is supported by the different insertions that have been observed in P. tricornutum accessions isolated from different locations around the world (Figure 6).
The significance of these findings is strengthened by the ecological relevance and common occurrence of stress in marine environments. Nitrogen is the most widespread limiting nutrient for marine phytoplankton , and transitions between nitrate starved stratified waters and nitrate replete upwelling conditions are a major influence governing marine diatom population oscillations . Conversely, diatom-derived unsaturated aldehydes can regulate intercellular signalling, stress surveillance, and defence against grazers [41–43]. Diatoms can sense these aldehydes accurately, whereby subthreshold levels serve as an early-warning protective mechanism, and lethal doses initiate a cascade leading to autocatalytic cell death. Activation of Surcouf only after exposure to high levels of aldehydes supports a threshold-dependent response in which activation only occurs under acute stress conditions. Furthermore, the fact that significant aldehyde concentrations are only produced by nutrient-stressed and wounded diatoms suggests a possible role in long term adaptation to abiotic and biotic stress [44–47].
Sexual reproduction in P. tricornutum has never been documented. Here, we have seen that Blackbeard and Surcouf insertions occurred at least a century ago and that both (as well as all other insertions tested; data not shown) have remained in a heterozygous state until now, in accordance with rare or absent meiotic cycles and only limited crossing overs between chromosome pairs in P. tricornutum. The maintenance of LTR-RT insertions in a heterozygous state in the P. tricornutum genome could increase the genetic variability between haplotypes and hence enhance adaptation capacity to changing environments. Furthermore, the observation that the Blackbeard element is hypomethylated in response to nitrate starvation provides a direct link between environmental stress and chromatin remodeling in diatoms. Such phenomena can confer phenotypic plasticity to an individual species, especially if they are heritable, and may be more useful for environmental adaptation than DNA-based modifications, which are irreversible and more likely to lead to speciation and therefore reproductive isolation. It is therefore possible that epigenetic modifications, combined with TE-mediated genomic rearrangements, maintain population diversity in P. tricornutum, as opposed to sex-driven chromosomal recombination. The potential capacity of such processes to monitor and to respond rapidly to changing environmental conditions may have contributed to the evolutionary and ecological success of diatoms in contemporary oceans.
Identification of transposable elements
TE complements from the P. tricornutum http://genome.jgi-psf.org/Phatr2/Phatr2.home.html and T. pseudonana http://genome.jgi-psf.org/Thaps3/Thaps3.home.html nuclear genomes were established by BLAST search  using the Repbase library  or single TE sequences, redundancy search and search for structural features such as ORFs larger than 1000 amino acids (which are characteristic of LTR-RT) and subsequent BLAST comparison with GenBank. When necessary, full length sequences were determined by examining multi-copy alignment. We then searched for the presence of LTRs upstream and downstream of the DNA sequence corresponding to the ORFs containing a polyprotein. LTR size sometimes varied by a few nucleotides between pairs and the length of the longest LTR is reported in Additional file 1. The target site duplication was examined in the genomic sequence directly flanking the LTRs. The DNA sequences between LTR pairs were translated in order to eventually identify another ORF (denoted ORF1 in Additional file 1) upstream of the ORF containing the polyprotein (ORF2). ORF2 and ORF1 were then submitted to InterProScan http://www.ebi.ac.uk/InterProScan/. The domain composition and order found in ORF2 was established by performing multiple alignments of the putatively active Ty1/copia-like elements from P. tricornutum and T. pseudonana with Ty1 from yeast and Copia from fruit fly and of the putatively active Ty3/gypsy-like elements from T. pseudonana with Gypsy from fruit fly, and Ty3 from yeast.
RT domains from P. multiseries, P. multistriata, F. cylindrus , O. tauri, C. reinhardtii, A. anophagefferens as well as from the GOS and Sargasso Sea metagenomic surveys were found using the RT amino acid sequences from the diatom LTR-RTs identified in this work and a set of RT domains assembled by Gao and collaborators (including elements from the Ty3/gypsy, Ty1/copia, DIRS, and BEL groups) as digital probes in BLAST searches  directly on the respective cDNA, genomic, and metagenomic databases.
Classification based on sequence similarity and structural features
We included the Blackbeard element in our analysis although it appears to be haplotype-specific and is absent from the final assembly of the P. tricornutum genome (see Results). The seven CoDi groups were divided into 26 distinct families on the basis of nucleotide pairwise distances. Further analysis of these elements revealed common structural features that were highly similar within multi-copy families (Additional file 1). Overall, the full length diatom retroelement sequences measure between 5182 bp (TpC22) and 8062 bp (PtC26). LTR length varies from 153 bp to 844 bp in the CoDi4.4 and CoDi3.2 families, respectively, and percent identity between LTR pairs varies from 94% to 100%, meaning that all the elements examined are likely to have inserted relatively recently in their respective genomes. The LTR TG/CA terminal inverted repeat is found in 23 out of 26 families and is missing only in CoDi3.2, CoDi4.2 and CoDi4.3. In some cases, such as the CoDi2.2 family, the terminal repeat is longer and contains up to 8 conserved nucleotides. The duplicated target site or direct repeat (DR) is quite heterogeneous within the groups although the P. tricornutum elements from the CoDi5 group consistently differ in a few A/T insertions between duplicates (for which the target site was found). Within the GAG-encoding region of these elements, InterProScan detected tandem CCHC zinc fingers in the elements belonging to the CoDi6.2-6.3-6.4-6.5 families (this domain is commonly found within this region of Ty1/copia-like elements).
The selected Ty3/gypsy-like elements from T. pseudonana represent two rather closely related groups called GyDi1 and GyDi2 (Ty3/ Gyp sy from D iatoms). Structural features of these elements are also shown in Additional file 1. We submitted one element from each family to GenBank (accession numbers are shown in Additional file 1).
Multiple alignments were performed with the CLUSTALW program . Genetic distances were calculated with the Poisson correction method  for amino acid sequences and phylogenetic trees were constructed with the Neighbor-Joining method . These evolutionary analyses were performed with the MEGA4 and SplitsTree4 platforms [54, 55].
In addition to the RT sequences identified in this work, phylogenetic trees presented in Figure 3 includes RT domains from Ty1/Copia, Ty3/Gypsy, DIRS and BEL LTR-RT lineages , as well as RT sequences from the Retroviridae human immunodeficiency virus type 1 (HIV), Rous sarcoma virus (RSV), and moloney murine leukaemia virus (MmLV) and from the Caulimoviridae Cauliflower mosaic virus (CaMV) and Rice tungro bacilliform virus (RtBV).
In addition to the sequences used in Figure 3 and 115 RT sequences from the CAMERA metagenomic database http://camera.calit2.net/, Figure 5 is built from a CLUSTALW alignment including also four RT sequences from C. reinhardtii, two from the O. tauri, one RT sequence from A. anophagefferens http://www.jgi.doe.gov/, and the RT sequence of the PyRE10G element from P. yezoensis (AB286055). For all phylogenetic analysis, the residues used were a modification of those originally identified by Toh et al. [57, 58] in retroviral, human hepatitis B virus (HBV), cauliflower mosaic virus (CaMV), and several retrotransposon sequences from Drosophila .
Cell culture and accessions
Axenic cultures of P. tricornutum Bohlin clone Pt1 8.6 (CCMP2561) were obtained from the culture collection of the Provasoli-Guillard National Center for Culture of Marine Phytoplankton, Bigelow Laboratory for Ocean Sciences, USA. Cultures were grown in f/2 medium  made with 0.2-μm-filtered and autoclaved local seawater supplemented with f/2 vitamins and inorganic nutrients (filter sterilized and added after autoclaving). Cultures were incubated at 18°C under cool white fluorescent lights at approximately 75 μmol.m-2.s-1 constant light and maintained in exponential phase in semi-continuous batch cultures. Sterility was monitored by occasional inoculation into peptone-enriched media to check for bacterial growth .
In order to evaluate the effect of nitrate stress on Blackbeard mRNA levels, cells were transferred to media modified with 50 μM NO3- and maintained in exponential phase in semi-continuous batch cultures. Samples were collected after 24 hrs and after 2 weeks exposure to nitrogen limitation. In order to evaluate the effect of diatom-derived reactive aldehydes on Surcouf transcript abundance, 2 liters of exponential P. tricornutum culture was treated with 2 μg/mL (2E,4EZ )-decadienal (DD) and control culture was treated with equivalent volume of methanol (DD solvent). Samples of 250 mL were collected in the indicated time points (0, 2, 6, 30, 96 hr) after exposure to DD treatment. (2E,4E/Z )-decadienal (DD) was obtained from Acros Organics USA. DD was dissolved in methanol, and concentrations were determined by measuring absorption at the lambda max for DD of 274 nm, using a Hewlett-Packard 8453 spectrophotometer. Diatom cells were harvested by centrifugation for 15 min at 3,000 g, washed with 12 mL of PBS, aliquoted into 2 mL Eppendorf tubes, and pelleted for 3 min at 10,000 g. Cell pellets were frozen instantly in liquid nitrogen and stored at -80°C before proceeding with RNA extraction.
The original sampling location of the P. tricornutum accessions Pt1-10 have been recently described in . We recently obtained three additional P. tricornutum accessions that we included for our SSAP analysis. Pt11 and Pt13 were sampled in 2008, respectively, in the Gulf of Naples and the Gulf of Salerno, Italy. Pt12 was obtained from the Roscoff culture collection.
SSAP experiments were conducted as previously described . Genomic DNA (500 ng) was digested with MspI and ligated to an MspI adaptor obtained by the annealing of two primers (Adap-MspI-C: 5'-CGT TCT AGA CTC ATC-3' and Adap-MspI-L: 5'-GAC GAT GAG TCT AGA A-3'). SSAP amplification was done by using a non labeled adaptor primer Msp1 (5'-GAT GAG TCT AGA ACG GC-3') and one of the following 33P-labelled LTR primers (Bkb, Scf and PtC34). Amplified products were separated on 6% denaturing polyacrylamide gels and exposed after drying to Kodak BioMax XAR films (Carestream Health Inc, Rochester). List of LTR primers: Bkb-Rev: 5'-ACG ATA ACC GAC CAG AAT CG-3' Scf-Rev: 5'-CCC GAA AAA CAT TGC CTC TA-3' PtC34-Rev: 5'-ATC GGA TCC AGG ACT TTG TG-3'
RNA purification and reverse transcription
mRNA levels of Blackbeard and Surcouf were analyzed using q-RT-PCR from triplicate samples collected from biological replicates of nitrate starved or DD-treated exponential grown cultures. Total RNA was extracted from approximately 108 cells using TRIzol Reagent (Invitrogen) and contaminating DNA was removed with TURBO DNase via treatment (Ambion), both according to manufacturer's protocols. RNA was then reverse transcribed into first strand cDNA with the SuperScript™ III First-Strand Synthesis System for RT-PCR (Invitrogen) using oligo-dT primers. Gene transcription was measured using the Brilliant® SYBR® Green QPCR Core Reagent Kit and the Stratagene MX3000P QPCR machine (Stratagene). Primers used for real-time PCR were Surcouf Fwd, 5'-CGA CCA CCG GCA TAC TTA TT-3', Surcouf Rev 5'-GGT TGT ACC GCA AGG CTA TG-3', Blackbeard Fwd 5'-GTG TTC TTG CTG CAA ATG GA-3', Blackbeard Rev 5'-ATT CAT CGG GGT CAC CAA TA-3', 18S rDNA Fwd 5'-CAT CCT TGG GTG GAA TCA GT-3' and 18S rDNA 5'-TGC GCA AAC CAA CAA AAT AG-3'. Additional primer sets were designed for Histone H4 and for TBP (TATA box binding protein) which served as a housekeeping gene for normalizing expression of the target gene . For each treatment, we evaluated each of the housekeeping genes and selected the one that showed the least amount of variation across conditions.
The pLTRbkb-GUS-FcpA plasmid was constructed from the FcpBp-GUS-FcpA vector  in which the FcpB promoter has been removed by KpnI/SalI digestion and replaced by ligation with a PCR fragment corresponding to Blackbeard LTR amplified using the Fwd 5'-CTT AGT GGT ACC TAG AAA AAC CCC ACG TCA AGC-3' and Rev 5'-CTT AGT GTC GAC GAT AAA CTA GAA AAC TGC AAC GAT AAC-3' and digested with KpnI/SalI. The pLTRbkb-GUS-FcpA vector was introduced into P. tricornutum by microparticle bombardment using a Biolistic PDS-1000/He Particle Delivery System (Bio-Rad, Hercules, CA, USA) as described by Falciatore et al. .
For β-glucuronidase (GUS) assays, 7 colonies carrying the pLTRbkb-GUS-FcpA construct were grown to mid-log phase in media containing 50 or 882 μM NO3-. Two weeks after cells were transferred to 50 μM NO3-, 20 ml cultures were collected by centrifugation at 3,800 rpm for 15 min at 4°C and resuspended in 120 μl freshly prepared GUS extraction buffer (50 mM NaPO4 pH 7.0, 10 mM β-mercaptoethanol, 0.1% Triton X-100), twice frozen in liquid nitrogen and thawed at 37°C, and finally centrifuged at 12,000 rpm for 5 min at 4°C. Soluble proteins were quantified with the Bio-Rad Protein Assay. The fresh extracts were used for spectrophotometric GUS assays performed by incubating at least 10 μg of total protein extract with the GUS enzyme substrate p-nitrophenyl glucuronide (PNPG) at a final concentration of 1 mM, in a total reaction volume of 1 ml. After a one hour incubation at 37°C, the colorimetric reaction was stopped by adding 0.4 ml 2.5 M 2-amino-2-methyl-1,3-propandiol and the absorbance measured at 415 nm. The enzymatic GUS activity was calculated on the base of the O.D. recorded and the molar extinction coefficient of the GUS substrate p-nitrophenol. One unit is defined as the quantity of enzyme that produces one nanomole of product in one minute at 37°C .
P. tricornutum cells grown were grown as described above under normal and nitrate-limited conditions for two weeks. DNA and RNA were extracted from 20 mL of culture for each condition. After cDNA synthesis from RNA samples (as described above) Blackbeard expression was verified by semi-quantitative RT PCR using the primers used for Q-PCR (see above) and primers amplifying the H4 and RPS housekeeping genes as controls . For McrPCR, 1 μg of DNA from each sample was incubated for 1 hour at 37°C with 20 units McrBC endonuclease supplemented with 100 μg/ml bovine serum albumin and 1 mM guanosine triphosphate. Negative controls were obtained with the same experimental procedure but replacing guanosine triphosphate with water. The enzyme was subsequently inactivated by incubation at 65°C for 10 minutes. Digestion efficiency of the Blackbeard locus was measured by semi-quantitative PCR using forward genomic primer -AAT ATT GGT CTT CGG CAA CG-3' and the Blackbeard-specific reverse primer 5'-GCT TCC GTC AAA CAC TCA CA-3' and we used the primers amplifying the H4 and RPS genes as controls (see above).
PCR haplotype/accession analysis
Polymerase chain reactions were performed using template DNA extracted from cultures of the ten different P. tricornutum accessions (see previous). The primers used to assess the presence of the two different haplotypes at the Blackbeard locus in DNA extracts from the ten accessions were the genomic Fwd 5'-AAT ATT GGT CTT CGG CAA CG-3' paired with the genomic Rev 5'-TTT GAC CCT ATT GGC TAC CG-3' or paired with the Blackbeard-specific Rev 5'-GCT TCC GTC AAA CAC TCA CA-3'. The primers used to assess the presence of the two different haplotypes at the Surcouf locus were the genomic Fwd 5'-TGT CTA TTG ACA TTT TGG AAG GTG-3'paired with the genomic Rev 5'-AGA TTC ATC AAT GGA TCA TCT CTC-3' or paired with the Surcouf-specific Rev 5'-GGG TAC CTG CTC CAT ATG TAG GTT-3'. Additional primer sets were designed for the other insertions analyzed.
We are grateful to Clémentine Vitte and Christian Parisod for helpful discussions and to Nicolas Maunoury, Agnès Meichenin, and Xin Lin for technical assistance. We are also thankful to Genoscope (Evry, France) for generating P. tricornutum ESTs, Uma Maheswari for the processing and preliminary analysis of EST data, Micaela S. Parker for the P. multiseries sequences, Alexander Luedeking and Uwe John for the P. multistriata EST sequences, and Jane Grimwood and Jeremy Schmutz for the consensus sequences corresponding to the Blackbeard haplotype.
- Kim JM, Vanguri S, Boeke JD, Gabriel A, Voytas DF: Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence. Genome Res. 1998, 8: 464-478.PubMedGoogle Scholar
- Kaminker JS, Bergman CM, Kronmiller B, Carlson J, Svirskas R, Patel S, Frise E, Wheeler DA, Lewis SE, Rubin GM, Ashburner M, Celniker SE: The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective. Genome Biol. 2002, 3: RESEARCH0084-10.1186/gb-2002-3-12-research0084.PubMed CentralView ArticlePubMedGoogle Scholar
- Kapitonov VV, Jurka J: Molecular paleontology of transposable elements in the Drosophila melanogaster genome. Proc Natl Acad Sci USA. 2003, 100: 6569-6574. 10.1073/pnas.0732024100.PubMed CentralView ArticlePubMedGoogle Scholar
- Quesneville H, Nouaud D, Anxolabehere D: Detection of new transposable element families in Drosophila melanogaster and Anopheles gambiae genomes. J Mol Evol. 2003, 57 (Suppl 1): S50-59. 10.1007/s00239-003-0007-2.View ArticlePubMedGoogle Scholar
- Smit AF: Interspersed repeats and other mementos of transposable elements in mammalian genomes. Curr Opin Genet Dev. 1999, 9: 657-663. 10.1016/S0959-437X(99)00031-3.View ArticlePubMedGoogle Scholar
- Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, Funke R, Gage D, Harris K, Heaford A, Howland J, Kann L, Lehoczky J, LeVine R, McEwan P, McKernan K, Meldrim J, Mesirov JP, Miranda C, Morris W, Naylor J, Raymond C, Rosetti M, Santos R, Sheridan A, Sougnez C: Initial sequencing and analysis of the human genome. Nature. 2001, 409: 860-921. 10.1038/35057062.View ArticlePubMedGoogle Scholar
- SanMiguel P, Gaut BS, Tikhonov A, Nakajima Y, Bennetzen JL: The paleontology of intergene retrotransposons of maize. Nat Genet. 1998, 20: 43-45. 10.1038/1695.View ArticlePubMedGoogle Scholar
- SanMiguel P, Tikhonov A, Jin JK, Motchoulskaia N, Zakharov D, Melake-Berhan A, Springer PS, Edwards KJ, Lee M, Avramova Z, Bennetzen JL: Nested retrotransposons in the intergenic regions of the maize genome. Science. 1996, 274: 765-768. 10.1126/science.274.5288.765.View ArticlePubMedGoogle Scholar
- Kumar A, Bennetzen JL: Plant retrotransposons. Annu Rev Genet. 1999, 33: 479-532. 10.1146/annurev.genet.33.1.479.View ArticlePubMedGoogle Scholar
- Xiong Y, Eickbush TH: Origin and evolution of retroelements based upon their reverse transcriptase sequences. EMBO J. 1990, 9: 3353-3362.PubMed CentralPubMedGoogle Scholar
- Round FE, Crawford RM, Mann DG: The Diatoms Biology and Morphology of the Genera. 1990, London, UK: Cambridge University PressGoogle Scholar
- Nelson DM, Treguer P, Brzezinski MA, Leynaert A, Queguiner B: Production and dissolution of biogenic silica in the ocean - Revised global estimates, comparison with regional data and relationship to biogenic sedimentation. Global Biogeochemical Cycles. 1995, 9: 359-372. 10.1029/95GB01070.View ArticleGoogle Scholar
- Raven JA, Waite AM: The evolution of silicification in diatoms: inescapable sinking and sinking as escape?. New Phytologist. 2004, 162: 45-61. 10.1111/j.1469-8137.2004.01022.x.View ArticleGoogle Scholar
- Falkowski PG, Katz ME, Knoll AH, Quigg A, Raven JA, Schofield O, Taylor FJ: The evolution of modern eukaryotic phytoplankton. Science. 2004, 305: 354-360. 10.1126/science.1095964.View ArticlePubMedGoogle Scholar
- Armbrust V, Berges J, Bowler C, Green B, Martinez D, Putnam N, Zhou S, Allenn A, Apt K, Bechner M, Brzezinski M, Chaal B, Chiovitti A, Davis A, Demarest M, Detter C, Glavina T, Goodstein D, Hadi M, Hellsten U, Hildebrand M, Jenkins B, Jurka J, Kapitonov V, Kroger N, Lau W, Lane T, Larimer F, Lippmeier C, Lucas S: The Genome of the Diatom Thalassiosira Pseudonana: Ecology, Evolution, and Metabolism. Science. 2004, 306: 79-86. 10.1126/science.1101156.View ArticlePubMedGoogle Scholar
- Bowler C, Allen AE, Badger JH, Grimwood J, Jabbari K, Kuo A, Maheswari U, Martens C, Maumus F, Otillar RP, Rayko E, Salamov A, Vandepoele K, Beszteri B, Gruber A, Heijde M, Katinka M, Mock T, Valentin K, Verret F, Berges JA, Brownlee C, Cadoret JP, Choi CJ, Coesel S, De Martino A, Detter JC, Durkin C, Falciatore A: The Phaeodactylum genome reveals the evolutionary history of diatom genomes. Nature. 2008, 456: 239-244. 10.1038/nature07410.View ArticlePubMedGoogle Scholar
- Kapitonov VV, Jurka J: Harbinger transposons and an ancient HARBI1 gene derived from a transposase. DNA Cell Biol. 2004, 23: 311-324. 10.1089/104454904323090949.View ArticlePubMedGoogle Scholar
- Smit AFA, Hubley R, Green P: RepeatMasker Open-3.0. 1996, [http://www.repeatmasker.org]Google Scholar
- Maheswari U, Mock T, Armbrust EV, Bowler C: Update of the Diatom EST Database a new tool for digital transcriptomics. Nucleic Acids Res. 2009, D1001-5. 10.1093/nar/gkn905. Epub 2008 Nov 23, 37 Database
- Pouteau S, Hunter E, Grandbastien MA, Caboche M: Specific expression of the tobacco Tnt1 retrotransposon in protoplasts. EMBO J. 1991, 10: 1911-1918.PubMed CentralPubMedGoogle Scholar
- Servant G, Pennetier C, Lesage P: Remodeling yeast gene transcription by activating the Ty1 long terminal repeat retrotransposon under severe adenine deficiency. Mol Cell Biol. 2008, 28: 5543-5554. 10.1128/MCB.00416-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Chandler VL, Walbot V: DNA modification of a maize transposable element correlates with loss of activity. Proc Natl Acad Sci USA. 1986, 83: 1767-1771. 10.1073/pnas.83.6.1767.PubMed CentralView ArticlePubMedGoogle Scholar
- Scortecci KC, Dessaux Y, Van Sluys MA: Somatic excision of the Ac transposable element in transgenic Arabidopsis thaliana after 5-azacytidine treatment. Plant Cell Physiol. 1997, 38: 336-343.View ArticlePubMedGoogle Scholar
- Miura A, Watanabe S, Toyama T, Shmada H, Kakutani T: Mobilization of transposons by a mutation abolishing full DNA methylation in Arabidopsis. Nature. 2001, 411: 212-214. 10.1038/35075612.View ArticlePubMedGoogle Scholar
- Liu ZL, Han FP, Tan M, Shan XH, Dong YZ, Wang XZ, Fedak G, Hao S, Liu B: Activation of a rice endogenous retrotransposon Tos17 in tissue culture is accompanied by cytosine demethylation and causes heritable alteration in methylation pattern of flanking genomic regions. Theor Appl Genet. 2004, 109: 200-209. 10.1007/s00122-004-1618-8.View ArticlePubMedGoogle Scholar
- Hashida SN, Uchiyama T, Martin C, Kishima Y, Sano Y, Mikami T: The temperature-dependent change in methylation of the Antirrhinum transposon Tam3 is controlled by the activity of its transposase. Plant Cell. 2006, 18: 104-118. 10.1105/tpc.105.037655.PubMed CentralView ArticlePubMedGoogle Scholar
- De Martino A, Meichenin A, Pan KH, Bowler C: Genetic and phenotypic characterization of Phaeodactylum tricornutum (Bacillariophyceae) accessions. Journal of Phycology. 2007, 43: 992-1009. 10.1111/j.1529-8817.2007.00384.x.View ArticleGoogle Scholar
- Devos KM, Brown JK, Bennetzen JL: Genome size reduction through illegitimate recombination counteracts genome expansion in Arabidopsis. Genome Res. 2002, 12: 1075-1079. 10.1101/gr.132102.PubMed CentralView ArticlePubMedGoogle Scholar
- Venter JC, Remington K, Heidelberg JF, Halpern AL, Rusch D, Eisen JA, Wu D, Paulsen I, Nelson KE, Nelson W, Fouts DE, Levy S, Knap AH, Lomas MW, Nealson K, White O, Peterson J, Hoffman J, Parsons R, Baden-Tillson H, Pfannkoch C, Rogers YH, Smith HO: Environmental genome shotgun sequencing of the Sargasso Sea. Science. 2004, 304: 66-74. 10.1126/science.1093857.View ArticlePubMedGoogle Scholar
- Rusch DB, Halpern AL, Sutton G, Heidelberg KB, Williamson S, Yooseph S, Wu D, Eisen JA, Hoffman JM, Remington K, Beeson K, Tran B, Smith H, Baden-Tillson H, Stewart C, Thorpe J, Freeman J, Andrews-Pfannkoch C, Venter JE, Li K, Kravitz S, Heidelberg JF, Utterback T, Rogers YH, Falcon LI, Souza V, Bonilla-Rosso G, Eguiarte LE, Karl DM, Sathyendranath S: The Sorcerer II Global Ocean Sampling expedition: northwest Atlantic through eastern tropical Pacific. PLoS Biol. 2007, 5: e77-10.1371/journal.pbio.0050077.PubMed CentralView ArticlePubMedGoogle Scholar
- Peddigari S, Zhang W, Takechi K, Takano H, Takio S: Two different clades of copia-like retrotransposons in the red alga, Porphyra yezoensis. Gene. 2008, 424: 153-158. 10.1016/j.gene.2008.07.021.View ArticlePubMedGoogle Scholar
- Terrat Y, Bonnivard E, Higuet D: GalEa retrotransposons from galatheid squat lobsters (Decapoda, Anomura) define a new clade of Ty1/copia-like elements restricted to aquatic species. Mol Genet Genomics. 2008, 279: 63-73. 10.1007/s00438-007-0295-0.View ArticlePubMedGoogle Scholar
- Piganeau G, Desdevises Y, Derelle E, Moreau H: Picoeukaryotic sequences in the Sargasso Sea metagenome. Genome Biol. 2008, 9: R5-10.1186/gb-2008-9-1-r5.PubMed CentralView ArticlePubMedGoogle Scholar
- Grandbastien MA, Spielmann A, Caboche M: Tnt1, a mobile retroviral-like transposable element of tobacco isolated by plant cell genetics. Nature. 1989, 337: 376-380. 10.1038/337376a0.View ArticlePubMedGoogle Scholar
- Bennetzen JL, Kellogg EA: Do Plants Have a One-Way Ticket to Genomic Obesity?. The Plant cell. 1997, 9: 1509-1514. 10.2307/3870439.PubMed CentralView ArticlePubMedGoogle Scholar
- Vicient CM, Suoniemi A, Anamthawat-Jonsson K, Tanskanen J, Beharav A, Nevo E, Schulman AE: Retrotransposon BARE-1 and Its Role in Genome Evolution in the Genus Hordeum. Plant Cell. 1999, 11: 1769-1784. 10.1105/tpc.11.9.1769.PubMed CentralView ArticlePubMedGoogle Scholar
- Wessler SR: Turned on by stress. Plant retrotransposons. Curr Biol. 1996, 6: 959-961. 10.1016/S0960-9822(02)00638-3.View ArticlePubMedGoogle Scholar
- Kalender R, Tanskanen J, Immonen S, Nevo E, Schulman AE: Genome evolution of wild barley (Hordeum spontaneum) by BARE-1 retrotransposon dynamics in response to sharp microclimatic divergence. Proc Natl Acad Sci USA. 2000, 97: 6603-6607. 10.1073/pnas.110587497.View ArticleGoogle Scholar
- Falkowski PG: Evolution of the nitrogen cycle and its influence on the biological sequestration of CO2 in the ocean. Nature. 1997, 387: 272-275. 10.1038/387272a0.View ArticleGoogle Scholar
- Smetacek V: Diatoms and the ocean carbon cycle. Protist. 1999, 150: 25-32. 10.1016/S1434-4610(99)70006-4.View ArticlePubMedGoogle Scholar
- Vardi A, Bidle KD, Kwityn C, Hirsh DJ, Thompson SM, Callow JA, Falkowski P, Bowler C: A diatom gene regulating nitric-oxide signaling and susceptibility to diatom-derived aldehydes. Curr Biol. 2008, 18: 895-899. 10.1016/j.cub.2008.05.037.View ArticlePubMedGoogle Scholar
- Vardi A, Formiggini F, Casotti R, De Martino A, Ribalet F, Miralto A, Bowler C: A stress surveillance system based on calcium and nitric oxide in marine diatoms. PLoS Biol. 2006, 4: e60-10.1371/journal.pbio.0040060.PubMed CentralView ArticlePubMedGoogle Scholar
- Ianora A, Miralto A, Poulet SA, Carotenuto Y, Buttino I, Romano G, Casotti R, Pohnert G, Wichard T, Colucci-D'Amato L, Terrazzano G, Smetacek V: Aldehyde suppression of copepod recruitment in blooms of a ubiquitous planktonic diatom. Nature. 2004, 429: 403-407. 10.1038/nature02526.View ArticlePubMedGoogle Scholar
- Fontana A, d'Ippolito G, Cutignano A, Romano G, Lamari N, Gallucci AM, Cimino G, Miralto A, Ianora A: LOX-induced lipid peroxidation mechanism responsible for the detrimental effect of marine diatoms on Zooplankton grazers. Chembiochem. 2007, 8: 1810-1818. 10.1002/cbic.200700269.View ArticlePubMedGoogle Scholar
- Ribalet F, Berges JA, Ianora A, Casotti R: Growth inhibition of cultured marine phytoplankton by toxic algal-derived polyunsaturated aldehydes. Aquatic Toxicology. 2007, 85: 219-227. 10.1016/j.aquatox.2007.09.006.View ArticlePubMedGoogle Scholar
- Ribalet F, Wichard T, Pohnert G, Ianora A, Miralto A, Casotti R: Age and nutrient limitation enhance polyunsaturated aldehyde production in marine diatoms. Phytochemistry. 2007, 68: 2059-2067. 10.1016/j.phytochem.2007.05.012.View ArticlePubMedGoogle Scholar
- Casotti R, Mazza S, Brunet C, Vantrepotte V, Ianora A, Miralto A: Growth inhibition and toxicity of the diatom aldehyde 2-trans, 4-trans-decadienal on Thalassiosira weissflogii (Bacillariophyceae). Journal of Phycology. 2005, 41: 7-20. 10.1111/j.1529-8817.2005.04052.x.View ArticleGoogle Scholar
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215: 403-410.View ArticlePubMedGoogle Scholar
- Jurka J, Kapitonov VV, Pavlicek A, Klonowski P, Kohany O, Walichiewicz J: Repbase Update, a database of eukaryotic repetitive elements. Cytogenet Genome Res. 2005, 110: 462-467. 10.1159/000084979.View ArticlePubMedGoogle Scholar
- Krell A, Gloeckner G: Analysis of an osmotic stress induced cDNA library of the psychrophilic diatom Fragilariopsis cylindrus. Public EST library deposited at NCBI in. 2004Google Scholar
- Higgins DG, Sharp PM: CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene. 1998, 73: 237-244. 10.1016/0378-1119(88)90330-7.View ArticleGoogle Scholar
- Nei M, Chakraborty R: Empirical relationship between the number of nucleotide substitutions and interspecific identity of amino acid sequences in some proteins. J Mol Evol. 1976, 7: 313-323. 10.1007/BF01743627.View ArticlePubMedGoogle Scholar
- Saitou N, Nei M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987, 4: 406-425.PubMedGoogle Scholar
- Kumar S, Tamura K, Nei M: MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform. 2004, 5: 150-163. 10.1093/bib/5.2.150.View ArticlePubMedGoogle Scholar
- Huson DH, Bryant D: Application of phylogenetic networks in evolutionary studies. Mol Biol Evol. 2006, 23: 254-267. 10.1093/molbev/msj030.View ArticlePubMedGoogle Scholar
- Gao X, Havecker ER, Baranov PV, Atkins JF, Voytas DF: Translational recoding signals between gag and pol in diverse LTR retrotransposons. RNA. 2003, 9: 1422-1430. 10.1261/rna.5105503.PubMed CentralView ArticlePubMedGoogle Scholar
- Toh H, Hayashida H, Miyata T: Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus. Nature. 1983, 305: 827-829. 10.1038/305827a0.View ArticlePubMedGoogle Scholar
- Toh H, Kikuno R, Hayashida H, Miyata T, Kugimiya W, Inouye S, Yuki S, Saigo K: Close structural resemblance between putative polymerase of a Drosophila transposable genetic element 17.6 and pol gene product of Moloney murine leukaemia virus. EMBO J. 1985, 4: 1267-1272.PubMed CentralPubMedGoogle Scholar
- Guillard RRL: Culture of phytoplankton for feeding marine invertebrates. Culture of Marine Invertebrate Animals. Edited by: Smith WLaCMH. 1975, New York, USA: Plenum PressGoogle Scholar
- Andersen RA, Morton S, Sexton J: CCMP - Provasoli-Guillard National Center for Culture of Marine Phytoplankton 1997 list of strains. Journal of Phycology. 1997, 33 (suppl): 1-75. 10.1111/j.0022-3646.1997.00001.x.View ArticleGoogle Scholar
- Petit M, Lim KY, Julio E, Poncet C, Dorlhac de Borne F, Kovarik A, Leitch AR, Grandbastien MA, Mhiri C: Differential impact of retrotransposon populations on the genome of allotetraploid tobacco (Nicotiana tabacum). Mol Genet Genomics. 2007, 278: 1-15. 10.1007/s00438-007-0226-0.View ArticlePubMedGoogle Scholar
- Siaut M, Heijde M, Mangogna M, Montsant A, Coesel S, Allen A, Manfredonia A, Falciatore A, Bowler C: Molecular toolbox for studying diatom biology in Phaeodactylum tricornutum. Gene. 2007, 406: 23-35.View ArticlePubMedGoogle Scholar
- Falciatore A, Casotti R, Leblanc C, Abrescia C, Bowler C: Transformation of Nonselectable Reporter Genes in Marine Diatoms. Mar Biotechnol (NY). 1999, 1: 239-251. 10.1007/PL00011773.View ArticleGoogle Scholar
- Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987, 6: 3901-3907.PubMed CentralPubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.