Table 3 Primers used in qRT-PCR analysis to determine expression patterns of ESTs from the Puccinia striiformis f. sp. tritici haustorial library in various developmental and infection stages
Primer | Sequence(5' to 3') | Products size (bp) |
|---|---|---|
RT-PSTha2a5-F | TGAATGGGTCGGTTGCCACAGATA | 180 |
RT-PSTha2a5-R | GGCCCAAAGGGAATGGTCGAATTT | Â |
RT-PSTha12j12-F | GCTTCGTTCGGGATTCAAAGCAAC | 138 |
RT-PSTha12j12-R | ACATCTTGGGAACAGGCAGTTTCG | Â |
RT-PSTha14i19-F | AAGTGCTCGAATGGGTCCTACCTT | 135 |
RT-PSTha14i19-R | TGTGACGTTCACTTAGCCGATCCA | Â |
RT-PSTha12h2-F | ACGTCAGTCAAAGATGTCGGCGAA | 120 |
RT-PSTha12h2-R | TTCCTATCAATTAGCGCGGGAGCA | Â |
RT-PSTha5a23-F | TTCCTACTCTGGCGACCAACATCA | 194 |
RT-PSTha5a23-R | AAATCCGACTGACCGACATCCGTT | Â |
RT-PSTha5a1-F | ACCGTATCGAAAGTGGTGTACGCT | 82 |
RT-PSTha5a1-R | TGTCGTCCATTGGTCCCATAGTGT | Â |
RT-PSTha12a4-F | GTTCACCAAAGCCACCTTCAACCA | 128 |
RT-PSTha12a4-R | ATTAGACGGCGGCGTTCTTAGGAT | Â |
RT-PSTha9F18-F | ATTCGAGATTAACGCGACCAACGG | 169 |
RT-PSTha9F18-R | GAAAGGTCAATGACAACGGCGTCT | Â |
RT-PSTha5G19-F | AGGTCTCGATTACCTTCCGCTTCT | 127 |
RT-PSTha5G19-R | AAGAAAGATCGAAACCAGCACCAG | Â |
RT-PSTha11n16-F | TGGGCATCTTCAGCTAGTTGGACT | 181 |
RT-PSTha11n16-R | TCAACACATTCAGACCACCTCCGA | Â |
RT-EF1-F | TTCGCCGTCCGTGATATGAGACAA | 159 |
RT-EF1-R | ATGCGTATCATGGTGGTGGAGTGA | Â |
RT-GAPDH-F | CAACGCTAGCTGCACCACTA | 161 |
RT-GAPDH-R | TTCCACCTCTCCAGTCCTTG | Â |
RT-TUB-F | CCGATCAATTCACGGCCATGTTCA | 174 |
RT-TUB-R | AACCCTCTTCAACTTCCTCGTCGT | Â |
RT-ACT-F | TGTCGGGTGGAACGACCATGTATT | 146 |
RT-ACT-R | AGCCAAGATAGAACCACCGATCCA | Â |