Figure 10
From: Differential gene expression in ADAM10 and mutant ADAM10 transgenic mice
Effect of Adam10 on Fabp7 and S100a8/a9 proteins in mouse brain. A) Fabp7 protein expression was analyzed in fractions of soluble brain proteins of mono-transgenic mice by Western blotting (Wt: wild-type, AD: ADAM10, dn: dominant negative ADAM10). As a control for antibody specificity, a lysate of HEK293 cells overexpressing V5-tagged murine Fabp7 (19 kDa; +) was used. B) Quantification of Fabp7 was performed with at least 5 animals per group. Values represent mean ± SEM, and values obtained for wild-type animals (Wt) were set to 100% (one way ANOVA, Bonferroni post-test; ns, not significant; p < 0.001, ***). C) Expression of dimeric S100a8/a9 (calprotectin) was quantified by ELISA in mouse brain extracts. Measured absorptions at 405 nm were normalized to wet tissue weight (mean ± SEM; n = 4).