Table 2 Conjugation frequencies of a subset of mini-Tn10::Km transposon mutants
From: Escherichia coli genes affecting recipient ability in plasmid conjugation: Are there any?
Recipient strain | Gene mutated and relevant features | A.L.U.a | Relative transfer rate in standard assay (% of wt)b |
|---|---|---|---|
No recipient | Â | -4.84 | <10-6 |
DH5α (pSU5024) |  | -2.57 | <10-6 |
108F7 | rfaC: Lipopolysaccharide biosynthesis | -1.53 | 6 |
129B10 | crp: DNA-binding transcriptional dual regulator | -1.32 | 59 |
183G6 | yejM: Hypothetical phosphatase/sulphatase | -1.32 | 96 |
94H11 | rfaD: Lipopolysaccharide biosynthesis | -1.21 | 13 |
152F1 | rfaC: Lipopolysaccharide biosynthesis | -1.07 | 20 |
130F12 | gppA: Guanosine pentaphosphatase/exopolyphosphatase | -1.07 | 16 |
13G7 | rfaD: Lipopolysaccharide biosynthesis | -1.03 | 23 |
121A4 | rfaD: Lipopolysaccharide biosynthesis | -1.02 | 22 |
144D7 | rfaD: Lipopolysaccharide biosynthesis | -0.97 | 6 |
11D1 | lpcA: Lipopolysaccharide biosynthesis | -0.94 | 15 |
31C2 | rfaC: Lipopolysaccharide biosynthesis | -0.92 | 26 |
149A8 | rfaP: Lipopolysaccharide biosynthesis | -0.79 | 21 |
68C12 | rfaP: Lipopolysaccharide biosynthesis | -0.76 | 32 |
110B7 | nhaA: integral membrane Na+/H+ antiporter | -0.74 | 41 |
7C12 | rfaG: Lipopolysaccharide biosynthesis | -0.60 | 19 |