Exploring and dissecting genome-wide gene expression responses of Penicillium chrysogenum to phenylacetic acid consumption and penicillinG production

Table 2 Physiological and microarray quality parameters for aerobic, glucose-limited chemostat cultures grown at a dilution rate of 0.03 h^-1

	DS17690 - PAA	DS17690 + PAA	DS50661 - PAA	DS50661 + PAA
Y _sx ^a (g·g ^-1 )	0.37 ± 0.01	0.35 ± 0.01	0.39 ± 0.01	0.36 ± 0.01
q _pen ^b (μmol·g ^-1 ·h ^-1 )	0.00 ± 0.00	19.81 ± 1.47	0.00 ± 0.00	0.00 ± 0.00
q _PAA ^c (μmol·g ^-1 ·h ^-1 )	0.00 ± 0.00	24.04 ± 2.38	0.00 ± 0.00	5.60 ± 0.38
q _CO2 ^d (mmol·g ^-1 ·h ^-1 )	1.15 ± 0.08	1.42 ± 0.11	1.16 ± 0.05	1.44 ± 0.10
q _O2 ^e (mmol·g ^-1 ·h ^-1 )	1.19 ± 0.11	1.42 ± 0.17	1.16 ± 0.11	1.43 ± 0.08
Avg CV ^f	0.21	0.18	0.17	0.14
Pc actA ^g	4190 ± 170	3560 ± 360	3450 ± 390	3030 ± 680
Pc gdh2 ^h	1240 ± 120	1140 ± 270	1020 ± 170	1060 ± 180
Repeats (n)	3	4	3	3

P. chrysogenum strains DS17690 (penicillinG high producing) and DS50661 (lacking a functional penicillin gene cluster) were grown in the presence and absence of phenylacetic acid (PAA) in independent glucose-chemostat cultures at D = 0.03 h^-1. Results are the averages ± S.D. (σ_n-1).
^a biomass yield on glucose (g of biomass per g of glucose consumed)
^b biomass specific penicillinG production rate. In addition to penicillinG, intermediates and byproducts are formed, which accounts for ~6% of the consumed PAA [30] (μmol of penicillin G produced per g of dry weight biomass and per · hour)
^c Biomass specific phenylacetate consumption rate (μmol of phenylacetate consumed per g of dry weight biomass and per hour)
^d Biomass specific CO₂ production rate (mmol of CO₂ produced per g of dry weight biomass and per hour)
^e Biomass specific O₂ consumption rate (mmol of O₂ consumed per g of dry weight biomass and per hour)
^f the average coefficient of variation (standard deviation divided by the mean) for all genes except the genes with mean below 12
^g encoding actin; average hybridization signal and standard deviation
^h encoding glutamate dehydrogenase (NAD⁺ dependent); average hybridization signal and standard deviation

ISSN: 1471-2164