Figure 3

Clusters of genes with similar expression profiles in somatic tissues during metamorphosis. Clusters were generated using gene expression data from male and female tud progeny at five time points during metamorphosis, indicated at top of clustergram. Expression profiles for each cluster were generated by averaging the gene expression data at each time point for every gene in the cluster in both sexes. Yellow and blue indicates high and low levels of expression compared to a common reference, respectively. To the right of the clustergram, black and grey indicates a cluster in which gene expression is at a peak (enriched) or at a trough (depleted), respectively, relative to the average expression value across all time points. Clusters are at a peak or trough of expression if average expression was 2/3 of a standard deviation above or below the mean expression value of the five time points, respectively. Functional annotation represents functional categories that were overrepresented among the genes in the cluster, as determined by the program DAVID (P < 0.05 [22]).