From: Comparing 2-nt 3' overhangs against blunt-ended siRNAs: a systems biology based study
Reaction Number | Reaction | Rate Constant | Remarks |
---|---|---|---|
1 | mRNA + siRNA -> gRNA | 0.008 hr-1 | Â |
2 | dsRNA -> 10*siRNA | 2.0 hr-1 | Â |
3 | mRNA -> dsRNA | 0.002 hr-1 | Â |
4 | siRNA ->Ï• | 2.0 hr-1 | Â |
5 | mRNA ->Ï• | 18 hr-1 | Â |
6 | gRNA ->Ï• | 2.8 hr-1 | Â |
7 | O + R -> O_R | 12.0 hr-1 | Regulatory molecule R binds to the operator region O to form the bound complex O_R |
8 | O_R -> O + R | 0.24 hr-1 | O_R dissociates into free R and O |
9 | P + RNAP -> P_RNAPC | 1.2 hr-1 | RNAP binds to promoter region P forming closed complex P_RNAPC |
10 | P_RNAPC -> P + RNAP | 0.06 hr-1 | P_RNAPC dissociates into free RNAP and P |
11 | P_RNAPC -> P_RNAPO | 48 hr-1 | Isomerization of closed to open complex (P_RNAPO) |
12 | P_RNAPO -> TrRNAP + mRNA + P | 54 hr-1 | RNAP clears promoter region and mRNA chain synthesis starts. TrRNAP denotes transcribing RNA polymerase. |
13 | TrRNAP -> RNAP | 4.8 hr-1 | RNAP completes transcription and is released from DNA. |
14 | mRNA + Ribosome -> RibRBS | 0.6 hr-1 | Ribosome binds to mRNA forming bound complex RibRBS |
15 | RibRBS -> mRNA+ Ribosome | 0.06 hr-1 | Ribosome dissociation from RibRBS |
16 | RibRBS -> EIRib + mRNA | 18 hr-1 | Ribosome EIRib initiates translation of mRNA chain |
17 | EIRib -> protein | 48 hr-1 | Protein synthesis by transcribing ribosome |
18 | protein ->Ï• | 0.18 hr-1 | Protein product degradation |