Figure 4
Activation of PPARγ represses MnSOD gene repression. (A) MDA-MB-231 and MDA-MB-468 cells were exposed to 5 μM and 10 μM 15 d-PGJ2 for 24 h before MnSOD mRNA and protein levels were assessed as described in M&M. Relative MnSOD mRNA expression is expressed as percent of untreated control. Data shown is the mean of three experiments done in duplicate +/- SD. MnSOD protein expression in (B) MDA-MB-231 and (C) MDA-MB-468 was analyzed by Western blot following 15 d-PGJ2 at doses indicated for 24 h. Equal loading ascertained using detection of β-actin