Figure 6From: Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of HumanPPARγ binds to PPRE3 in human MnSOD promoter. (A) Schematic representations of full length human MnSOD promoter construct (-3400 to +24 pGL3) and two 5'-deletion constructs (-1605 to +24 pGL3; -555 to +24 pGL3) with location of PPRE1, PPRE2 and PPRE3 indicated. (B) Luciferase assay of MnSOD promoter activity in MDA-MB-231 transiently transfected with full length and 5'-deletion derivatives followed 5 μM and 10 μM 15 d-PGJ2 treatment for 16 h. Renilla plasmid was cotransfected to assess transfection efficiency. Results are expressed as RLU/renilla/μg total protein. Data represents the average +/-SD of two experiments done in duplicate. (C) No-shift DNA binding assay to assess DNA binding activity of PPARγ at PPRE2 and PPRE3 of MnSOD promoter as described in M&M. Results are expressed as absorbance readings at 450 nm. Data represents the average +/- SD of two experiments done in duplicate.Back to article page