Comparative gene expression profiling between human cultured myotubes and skeletal muscle tissue
© Raymond et al; licensee BioMed Central Ltd. 2010
Received: 30 June 2009
Accepted: 22 February 2010
Published: 22 February 2010
A high-sensitivity DNA microarray platform requiring nanograms of RNA input facilitates the application of transcriptome analysis to individual skeletal muscle (SM) tissue samples. Culturing myotubes from SM-biopsies enables investigating transcriptional defects and assaying therapeutic strategies. This study compares the transcriptome of aneurally cultured human SM cells versus that of tissue biopsies.
We used the Illumina expression BeadChips to determine the transcriptomic differences between tissue and cultured SM samples from five individuals. Changes in the expression of several genes were confirmed by QuantiGene Plex assay or reverse transcription real-time PCR. In cultured myotubes compared to the tissue, 1216 genes were regulated: 583 down and 633 up. Gene ontology analysis showed that downregulated genes were mainly associated with cytoplasm, particularly mitochondria, and involved in metabolism and the muscle-system/contraction process. Upregulated genes were predominantly related to cytoplasm, endoplasmic reticulum, and extracellular matrix. The most significantly regulated pathway was mitochondrial dysfunction. Apoptosis genes were also modulated. Among the most downregulated genes detected in this study were genes encoding metabolic proteins AMPD1, PYGM, CPT1B and UCP3, muscle-system proteins TMOD4, MYBPC1, MYOZ1 and XIRP2, the proteolytic CAPN3 and the myogenic regulator MYF6. Coordinated reduced expression of five members of the GIMAP gene family, which form a cluster on chromosome 7, was shown, and the GIMAP4-reduction was validated. Within the most upregulated group were genes encoding senescence/apoptosis-related proteins CDKN1A and KIAA1199 and potential regulatory factors HIF1A, TOP2A and CCDC80.
Cultured muscle cells display reductive metabolic and muscle-system transcriptome adaptations as observed in muscle atrophy and they activate tissue-remodeling and senescence/apoptosis processes.
Oligonucleotide microarrays can reveal gene expression profiles of SM tissue and provide valuable insight into molecular pathways involved in pathogenesis or abnormally regulated in disease. Various human disorders that affect the SM tissue have been analyzed using microarray technology, including disuse atrophy , myositis [2, 3], Duchenne muscular dystrophy (DMD) [4–6] and others [7–9].
One of the limitations of applying microarray analyses to such context is the amount of tissue material needed . For SM studies, genome-wide expression profiling has in some cases been applied to pooled samples from multiple patients [4, 7]; in others, microarrays with a restricted number of genes were used [2, 6, 8, 9]. The possibility of performing whole transcriptome microarray analysis on small amounts of tissue material facilitates its application to tissue samples from individual subjects.
The culturing of myotubes from SM biopsies opens an alternative to examine transcriptional defects with microarrays and enables therapeutic strategies to be assayed. SM cell cultures can be established by the explant technique , which is based on the presence of myogenic progenitors, the satellite cells . Satellite cells are not committed to fiber type lineages [13, 14]. When satellite cells are stimulated with growth factors, they generate myoblasts, which can replicate a limited number of times and be induced to fuse and form multinucleated myotubes. The drawback of cell cultures in general is that cells change their phenotype, which may alter the expression of certain genes and compromise the phenotypic expression of the disease. Indeed, aneurally cultured human SM cells remain relatively immature as shown in studies of the protein isoform pattern [14–16] and microarray analysis of the transcriptome .
There were no data on transcriptome differences between cultured and tissue human SM cells. We deployed an automated high-sensitivity microarray platform to identify genes differentially expressed between aneurally cultured myotubes derived from human SM biopsies by the explant technique and the SM tissue samples. We provide insight into the phenotype of the cultured human SM, which is a valuable cell model for pathogenesis studies and therapeutic assays.
Skeletal muscle specimens and cultures
Paravertebral muscles biopsies were obtained from five subjects: females of age 12-15 years devoid of neuromuscular disease during surgery for idiopathic scoliosis. Informed consent and approval from the Ethics Committee of the Hospital Sant Joan de Déu (Barcelona) was obtained. Biopsies were embedded into RNAlater for RNA extraction or into cell culture medium. Cultures were prepared through an explant technique [11, 17]. Briefly, SM biopsy pieces were dissected under a stereomicroscope; fat, connective tissue and blood were removed; and the pieces were frozen in DMEM medium with 25% fetal bovine serum (FBS) and 6% DMSO. To set up the culture, first the biopsy pieces were spaced embedded in a semi-solid matrix, composed of 5 ml of DMEM/M-199 medium (3:1) with 37.5% FBS and 1.25 ml of human plasma (Sigma-Aldrich, St. Louis, MO, USA), overspread onto tissue culture plates, which were then incubated for 6 to 8 days to permit fibroblast outgrowth. The biopsy pieces were then removed from the matrix with forceps under the stereomicroscope, dissected and embedded in a matrix, composed of 1 ml of human plasma and 2 ml of 1.5% gelatin, spotted and stuck onto new dishes and overlaid with DMEM/M-199 medium (3:1) with 10% FBS, 10 μg/ml insulin, 4 mM glutamine, 25 ng/ml fibroblast growth factor, and 10 ng/ml epidermal growth factor, for 5 to 7 days to permit myoblast proliferation and migration. After myoblast generation, the biopsy pieces were removed, and eventually re-explanted, and myoblast monolayers were dissociated with trypsin and subcultured. Myogenic cells from the same explant were subjected to limited consecutive subcultures to avoid differences resulting from senescence . Myotubes were derived from confluent myoblast cultures; immediately after initiation of myoblast fusion, medium was replaced by DMEM/M-199 medium (3:1) with 10% FBS and 10 μg/ml insulin, to further stimulate differentiation. Myotubes were used 7 days later. Myotube preparations were labeled B19, B22, B24, B25 and B26.
Cells grown on coverslips were fixed in 4% paraformaldehyde in PBS for 15 min, then washed in PBS, and incubated for 10 min in PBS containing 50 mM NH4Cl, 10 min in PBS containing 20 mM glycine, 10 min in PBS containing 0.1% Triton X-100 and 30 min in PBS containing 10% FBS. Subsequently coverslips were incubated for 1 h at room temperature with a rabbit anti-desmin antibody (1:100; AB907 Millipore, Billerica, MA, USA). Primary antibody was detected with an Alexa Fluor-488 goat anti-rabbit antibody (1:500; Molecular Probes, Eugene, OR, USA). Both primary and secondary antibodies were diluted in blocking solution. Nuclei were stained with Hoescht (1 μg/ml) during secondary antibody incubation. After staining, samples were mounted on Mowiol mounting medium and analyzed with a Leica TCS SP2 confocal microscope. Images were processed using Photoshop CS software (Adobe Corp, San Jose, CA, USA). From 200 to 500 nuclei in each of the 5 myotube cultures were analyzed with ImageJ (Rasband WS, ImageJ, National Institutes of Health, Bethesda, MD, USA, http://rsb.info.nih.gov/ij/ and the mean ± SD values of the percentage of nuclei located in desmin-labeled myotubes were calculated.
Gene expression analysis
Ten samples, 5 from cultured SM cells, and 5 from SM biopsies were analyzed by DNA microarrays. Total RNA was extracted from SM biopsies with RNeasy fibrous tissue kit and homogenized using a TissueLyser, and from cultured myotubes with RNeasy kit (Qiagen, Valencia, CA, USA), with the same DNase treatment than in the RNeasy fibrous tissue kit, and homogenized with a Polytron. RNA samples were quantified with the RiboGreen RNA Quantification Kit (Molecular Probes), and monitored with the Agilent 2100 Bioanalyzer, to check high-quality RNA (RNA integrity = 8). We used HumanRef-8 v2.0 Expression BeadChips (Illumina, San Diego, CA, USA), which comprise probes to interrogate 22200 transcripts, based on the curated content of the NCBI Reference Sequence database, release 17.
An aliquot of 150 ng of total RNA was used to produce double-stranded cDNA, followed by transcription in vitro, and by cRNA labeling with biotin using the TotalPrep RNA Amplification kit (Applied Biosystems/Ambion, Austin, TX, USA) recommended by Illumina. This method is based on the protocol developed in J. Eberwine's laboratory . The procedure consists of reverse transcription with an oligo(dT) primer bearing a T7 promoter using a reverse transcriptase engineered to produce high yields of first strand cDNA. The reverse transcriptase catalyzes the synthesis of full-length cDNA which then undergoes second strand synthesis and clean-up to become a template for in vitro transcription (IVT) with T7 RNA Polymerase. The IVT, along with biotin UTP, is used to generate hundreds to thousands of biotinylated, antisense RNA copies of each mRNA. These procedures were performed on an automated system primarily developed for the preparation of samples for Affymetrix arrays , which we adapted for the Illumina procedure. It is composed by a Microlab Star liquid handling system (Hamilton, Bonaduz, Switzerland) for reagent and sample pipetting and mixing, coupled with a thermocycler TRobot (Biometra, Goettingen, Germany) for incubations, and a microtiter plate reader SpectraMax M2 (Molecular Devices, Sunnyvale, CA, USA) for nucleic acids quantification with RiboGreen assay. The 10 samples analyzed in the present experiment were processed in a single robotic session. The labeled-cRNAs thus produced were measured with the Agilent 2100 Bioanalyzer to control for length. All cRNA sizes were close to 1200 nt and therefore eligible for hybridization onto the microarrays. Then, 750 ng of labeled-cRNAs was added to the hybridization mix, which contained control oligonucleotides in hybridization buffer. Then, 15 μl of each hybridization mix was dispensed on the BeadArrays. After hybridization (16 h, 58°C), the arrays were washed to remove non-hybridized material and were stained with streptavidin-Cy3, which bound to biotin. Scanning was performed using the BeadArray Reader, which provided intensity values for all transcripts. Bead redundancy allowed the calculation of a detection p-value which served at declaring the transcripts "significantly detected" or not. Signal intensities were extracted and summarized in the BeadStudio software. Data were expressed as absolute intensities, to which we applied a background correction. Background was calculated for each array by the average signal of the negative control probes. All microarray experiments were run at the same time. The Illumina HumanRef-8 BeadChip is a glass slide comprising 8 identical microarrays. We therefore used 2 slides for this experiment. To minimize possible batch effect that can occur between the two glass slides, we randomized the sample distribution in the following order: in one slide A (B19 in vitro), B (B26 in vitro), C (B25 in vivo), D (B24 in vitro), E (B24 in vivo), F (B22 in vivo), G (B26 in vivo) and H (B25 in vitro) and in the other slide A (B19 in vivo) and B (B22 in vitro). "In vitro" stands for cultured myotubes and "in vivo" means SM tissue biopsies. As shown in a recent study , randomization on Illumina slides greatly improves the results reducing the number of possible false positive occurrences that would lead to a misinterpretation of the data. Microarray data files have been deposited in GEO Omnibus [http://www.ncbi.nlm.nih.gov/geo/ GEO accession number GSE17503].
QuantiGene Plex 2.0 assay (Panomics/Affymetrix, Fremont, CA, USA) was used for confirmation of the microarray analysis. The advantage of this assay is that no RNA amplification is needed, unlike microarrays or quantitative PCR. A comparison to other gene expression platforms in the frame of the MAQC project demonstrated the sensitivity and accuracy of QuantiGene techniques . This technology uses three sets of pooled oligonucleotides that function to capture, provide binding sites for signal amplification molecules, and stabilize the mRNA of interest. The capture extender (CE) pool contains oligonucleotides that are complementary to the mRNA of interest and to capture probes (CPs) covalently linked to carboxylated fluorescent-encoded micro spheres (Luminex, Austin, TX, USA). The label extender (LE) pool has oligonucleotides that contain sequences complementary to the mRNA of interest and to the amplifier molecules which carry a streptavidin phycoerythrin (SAPE) conjugate. The blocker (BL) pool contains oligonucleotides that are complementary to the mRNA where neither CE nor LE bind and it protects the mRNA by maintaining an intact RNA-DNA hybrid. The micro spheres (or beads) thus prepared are analyzed with a Luminex reader, which utilizes two lasers, the first for bead identification, measuring the fluorescent signal specific to each bead type, and the second for mRNA quantification, determining the fluorescence level of SAPE and therefore the expression level of the transcript. The assay was performed according to the manufacturer's instructions, accessible at: http://panomics.com/downloads/UM13075_RevB_QGP2_080430.pdf. Briefly, the RNAs extracted from tissue and cell samples were deposited on a 96-well plate along with the QuantiGene hybridization solution (containing RNase-free water, lysis mixture, blocking reagent, proteinase K, capture beads and probe set). The hybridization plate was then sealed and placed in a shaking incubator (Vortemp 56 Shaker/Incubator, Labnet International, Inc., Woodbridge, NJ, USA) at 54°C and 600 rpm for overnight hybridization (18-22 h). After centrifugation to remove condensation, the hybridization mixture was transferred to a pre-wetted filter plate, where the mixture was filtered and washed three times in wash buffer. All washing steps were performed with a MultiScreen Vacuum Manifold (Millipore) at low pressure, and after the final wash, the filter plate bottom was blotted to remove any remaining liquid to prevent leakage. The samples were then incubated successively in three working solutions: pre-amplifier and amplifier, biotinylated probes and SAPE. Two washes were performed between each incubation to remove unbound reagents. The plate was covered with aluminum foil during SAPE incubation to prevent photo bleaching. For Luminex measurement, after a calibration of the Luminex, the plate was placed in the instrument. Prior to the measurement, each bead type was identified in the Luminex software in order to specify to which transcripts the beads corresponded. At the start of the measurement, the content of each well was aspirated and pushed towards the lasers. As occurs in a flow cytometer, the beads passed successively in front of the two lasers. Beads were identified, and fluorescent signals were measured. The final outcome was a raw data file containing signal intensity and background level for each gene in each sample. Data were analyzed using the transferrin receptor (TFRC) gene as the reference gene, since its expression was similar within the two examined groups, cultured myotubes and SM biopsies.
For reverse transcription (RT) and real-time PCR, an aliquot of 0.5 μg of total RNA was retro-transcribed (RT) with TaqMan reverse transcription reagents from Applied Biosystems (Foster City, CA, USA) using random hexamers and in a 25 μl total volume reaction. Real-time PCR was performed on 1 μl of the RT reaction mixture with the TaqMan universal PCR master mix and TaqMan Gene Expression Assays using the ABI PRISM 7700 sequence detection system. Probes for PYGM (Hs00194493_m1), UCP3 (Hs00243297_m1), THY1 (Hs00264235_s1) and B2M (Hs99999907_m1) were from Applied Biosystems. The threshold cycle (Ct) values of the reference probe beta-2 microglobulin (B2M) were subtracted from each target probe Ct values (ΔCt), the ΔCt values of the SM biopsies were subtracted from the ΔCt values of myotube cultures. Data were expressed as mean values of 2-ΔΔCT for upregulated genes and 1/2-ΔΔCT for downregulated ones and the significance of differences was estimated by a paired t-test.
Statistical analysis and filtering
After the scanning of the microarrays, BeadStudio generated a dendrogram to control for the sample clustering. Subsequent steps of quality control (QC), statistical analysis and filtering of microarray data were carried out with GeneSpring GX software v10.0.2 (Agilent Technologies, Santa Clara, CA, USA). Raw data with background subtracted were loaded from Illumina BeadStudio to GeneSpring GX. This step was followed by quantile normalization and log2 transformation. The samples were then grouped into two conditions which we called "in vitro" and "in vivo", corresponding respectively to the SM cultures and the tissue biopsies. GeneSpring GX was used as the first step for QC of data on all the samples with two unsupervised methods, Pearson correlation and Principal Component Analysis (PCA). The PCA vector space transformation was used to reduce multidimensional data sets to lower dimensions for analysis to assess the behavior of the samples and to identify or confirm possible outliers. Within the quality control step, Genespring GX enables to compute the 3 first principal components. The percentage of total variance that each of these 3 principal components capture is shown in the legend of Additional File 1.
To assess which genes were differentially expressed between the two conditions examined, we performed a corrected paired t-test on all probes. Because of the small number of biological replicates, we used the Benjamini-Hochberg false discovery rate (FDR) method controlling for false positives, in order to select genes with the lowest FDR. This method is less stringent than other corrections such as the Bonferroni, and provides a good balance between discovery of statistically significant genes and limitation of false positive occurrences. A corrected p-value cutoff of 0.01 was used to select the regulated genes with the lowest FDR. A fold change value was also computed by GeneSpring GX to assess the level and the direction of the gene regulation. Fold change was calculated as the absolute ratio of normalized intensities between the mean values of the samples in the two conditions, cultured myotubes and SM tissue biopsy, grouped.
Data from QuantiGene Plex were also loaded into GeneSpring GX software. Originally GeneSpring GX was designed for gene expression microarray experiments and uses the main commercially available arrays (Affymetrix, Illumina, Agilent and CodeLink). However, it is possible to configure a "custom" array. The QuantiGene Plex assay was therefore configured in GeneSpring GX. After normalization to the TFRC control gene, data were log2 transformed, and significance of differential expression was assessed by a paired t-test.
Functional annotation and classification analysis was also carried out with GeneSpring GX program, running a Gene Ontology (GO) analysis on the gene set selected with the statistical analysis described above. GeneSpring GX computed a p-value to quantify the significance in the GO analysis. This p-value is the probability that a random subset of x genes drawn from the total set of n genes will have y or more entities containing a given GO term. This probability is described by a standard hypergeometric distribution. GeneSpring GX uses the hypergeometric formula from first principles to compute this probability. Since a large number of hypotheses will be tested, some form of correction is required. GeneSpring GX addresses this issue using the Benjamini-Yekutieli correction, which takes into account the dependency among the GO terms. In the GO analysis done in GeneSpring GX, a low p-value therefore implies that the given GO term is enriched. The Gene Ontology data in GX 10.0.2 is compiled from version 1.2 of OBO (Open Biomedical Ontologies).
Data were interpreted using Ingenuity Pathways Analysis (IPA) (Ingenuity Systems, Redwood City, CA, USA) http://www.ingenuity.com. The list of significantly regulated genes selected by the microarray analysis described above was loaded in IPA with the following criteria: Reference set: HumanRef-8 v2.0; Direct and Indirect relationships included; filtered by species (human), and in a second round, by tissue (skeletal muscle). Then IPA computed the data to generate significant networks of genes that are associated with particular biological functions, diseases, and molecular processes. It showed also the canonical pathways stimulated by the experiment. IPA used the right-tailed Fisher's exact test to extract significant pathways. IPA canonical pathways are well-characterized metabolic and cell-signaling pathways coming from articles, reviews, books, and KEGG Ligand.
Gene cluster analysis was performed with the REEF (REgionally Enriched Features in genomes) program, which scans the genome using a sliding window approach, and calculates the statistical significance of each window using the hypergeometric distribution and the false discovery rate .
Genes differentially expressed in cultured versus skeletal muscle tissue
Microarray analyses were performed on the 5 SM cultures and the 5 SM biopsies. We confirmed that myotubes were the prevalent cell type in each of the SM cultures by immunostaining for desmin, a muscle-specific intermediate filament protein  (Additional file 1, Figure S1). Most of the Hoescht-stained nuclei were located in desmin-labeled myotubes: B19 (78.3 ± 3.12%), B22 (61.4 ± 6.60%), B24 (61.8 ± 8.36%), B25 (83.6 ± 14.50%) and B26 (79.7 ± 5.05%). The microarray analysis showed that in the cultured myotubes compared to the tissue biopsies 1260 transcripts, which correspond to 1216 nuclear genes, were differentially expressed (selection criteria: absolute fold-change value > 2 with a corrected p-value of p < 0.01). Of these, 583 were downregulated and 633 were upregulated. The complete list of regulated genes is shown in Additional file 2, Table S1. On the basis of the correlation analysis performed, Additional file 3, Figure S2 shows that the samples were clearly separated based on their condition cultured myotubes or SM biopsy. The dendrogram also illustrates that the paired samples were differently distributed in the two conditions. PCA and correlation matrix procedures show that the two experimental conditions were well separated and there were no particular outliers in the experiment. Moreover, they highlight that the variability was higher within the SM culture group (in vitro). Pearson coefficients are also provided; the average Pearson coefficient was 0.97 for the SM biopsies and 0.93 for the SM cultures. PCA, correlation matrix and dendrogram also confirmed that there was no significant batch effect between the two Illumina slides in our experiment. In an attempt to restrict differentially expressed genes to those expressed in SM, microarray data were filtered according to the IPA knowledge-base, so that genes expressed in SM were selected. SM-specificity is shown in Additional file 2, Table S1. IPA knowledge about mRNA expression in tissues is based on two sources: the GNF body atlas , and literature findings. Among the downregulated genes, 384 passed this filter along with 318 of the upregulated genes. The filtering served as a means for identifying genes that are expressed in other cell types, present in the SM tissue, within the most regulated genes. For instance, hemoglobin HBB and HBA2 genes, which are mainly expressed in erythroid cells , from the most downregulated group and the THY1 gene, which is mainly expressed in human fibroblasts, neurons and endothelial cells , from the most upregulated group. Remarkably, the ratios between the normalized signal of the THY1 and desmin gene markers in the SM cultures were in a narrow range: B19 (1.94), B22 (1.12), B24 (1.42), B25 (1.28) and B26 (1.44), again indicating that fibroblast presence did not vary widely within SM cultures. However, the THY1/desmin ratios were from 1.3- to 3.2-fold higher in the SM cultures than in the SM biopsies.
The complete set of differentially expressed genes were classified, separately for downregulated and upregulated genes, according to GO (Additional file 4, Table S2A). With respect to the cellular component, genes that were downregulated in cultured myotubes were mainly associated with cytoplasm and enriched in mitochondria. The over-represented biological processes were metabolism, with the largest group of regulated genes, and muscle-system/contraction. With respect to metabolism, genes were mainly involved in the generation of precursor metabolites and energy, cellular respiration and oxidative phosphorylation; quinone cofactor metabolism was also affected. The most augmented molecular function was oxidoreductase activity. Among the genes that were upregulated in cultured myotubes, the enriched cellular components were cytoplasm (with the largest group associated with endoplasmic reticulum (ER)) and the extracellular matrix. The enriched molecular function here was oligosaccharyl transferase activity. The augmented biological process was modification of an amino acid residue in a protein: N-linked posttranslational glycosylation at asparagine residues. GO annotations of the SM-expressed subset of downregulated genes (Additional file 4, Table S2B) did not differ greatly from those of the whole gene set, except for the occurrence of the following over-represented GO annotations: cytoskeletal protein binding (molecular function); fatty acid beta-oxidation and organic acid metabolism-related terms (metabolic processes); and muscle development (biological process). There was no GO annotation enrichment within the upregulated SM-expressed subset for molecular function and biological process categories (Additional file 4, Table S2B).
Top regulated genes filtered by expression in skeletal muscle
carbonic anhydrase III, muscle specific
tropomodulin 4 (muscle)
myosin, light chain 3, alkali; ventricular, skeletal, slow
calsequestrin 1 (fast-twitch, skeletal muscle)
adenosine monophosphate deaminase 1 (isoform M)
myosin binding protein C, slow type
ankyrin repeat and SOCS box-containing 10
chloride intracellular channel 5
S100 calcium binding protein A1
ribosomal protein L3-like
ankyrin repeat domain 23
nebulin-related anchoring protein
fibronectin type III and SPRY domain containing 2
carbonic anhydrase II
phosphorylase, glycogen; muscle (McArdle syndrome, glycogen storage disease type V)
actin binding LIM protein family, member 2
myogenic factor 6 (herculin)
ankyrin repeat and SOCS box-containing 12
transmembrane protein 52
xin actin-binding repeat containing 2
ankyrin repeat domain 2 (stretch responsive muscle)
complement component 1, q subcomponent, A chain
aldehyde dehydrogenase 1 family, member L1
integral membrane protein 2A
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
calpain 3, (p94)
myocilin, trabecular meshwork inducible glucocorticoid response
nipsnap homolog 3B (C. elegans)
monoamine oxidase B
carnitine palmitoyltransferase 1B (muscle)
potassium voltage-gated channel, shaker-related subfamily, member 7
pentraxin-related gene, rapidly induced by IL-1 beta
cyclin-dependent kinase inhibitor 1A (p21, Cip1)
transmembrane protein 200A
pleckstrin and Sec7 domain containing 3
solute carrier family 38, member 10
TIMP metallopeptidase inhibitor 2
topoisomerase (DNA) II alpha 170kDa
procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
chondroitin sulfate glucuronyltransferase
transmembrane protein 119
procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
glutathione peroxidase 8
hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
collagen triple helix repeat containing 1
PDZ and LIM domain 4
coiled-coil domain containing 80
matrix metallopeptidase 14 (membrane-inserted)
neurofascin homolog (chicken)
centrosomal protein 55kDa
serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
DnaJ (Hsp40) homolog, subfamily C, member 10
sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
glucosidase, beta; acid (includes glucosylceramidase)
vitamin D (1,25- dihydroxyvitamin D3) receptor
sphingomyelin synthase 2
Among the most upregulated genes detected in this study (Table 1) are well-known regulatory factors such as CDKN1A/p21, TOP2A and HIF1A. Several genes are related to the extracellular matrix, such as those encoding: the Ca(2+)-binding proteoglycan SPOCK1; isoforms of lysyl hydroxylase (PLOD3, PLOD2) , an enzyme that catalyzes the formation of hydroxylysine in collagens; CSGlcA-T, which is involved in the synthesis of chondroitin sulphate as a glucuronyltransferase ; the metalloproteinase MMP14; TIMP2, an inhibitor of the metalloproteinase type IV collagenolytic activity  and the protease inhibitor SERPINE2 . The NFASC gene, which promotes axon subcellular targeting and synapse formation , was another highly induced gene, suggesting stimulation of neuronal signaling. Two genes are related to stress: the gene encoding the putative glutathione peroxidase GPX8, an enzyme involved in protection against oxidative stress , and the DNAJC10/ERDJ5 gene. The PTX3 gene is related to inflammation . Other genes are related to sphingomyelin metabolism such as those encoding: GBA, a lysosomal enzyme that catalyzes glucosylceramide breakdown , and SGMS2, an enzyme that produces sphingomyelin . Finally, a group of genes encodes proteins that either have an undefined (i.e. TMEM200A), or an unknown function in the SM tissue (KIAA1199, PSD3, CCDC80/URB/SSG1/DRO1 and LPIN2).
Genes validated for differential expression
tropomodulin 4 (muscle)
adenosine monophosphate deaminase 1 (isoform M)
myosin binding protein C, slow type
phosphorylase, glycogen; muscle
myogenic factor 6 (herculin)
xin actin-binding repeat containing 2
calpain 3, (p94)
carnitine palmitoyltransferase 1B (muscle)
uncoupling protein 3 (mitochondrial, proton carrier)
GTPase, IMAP family member 4
Thy-1 cell surface antigen
coiled-coil domain containing 80
hypoxia-inducible factor 1, alpha subunit
topoisomerase (DNA) II alpha 170kDa
cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Bioinformatic analysis and interpretation of microarray expression data
Significantly regulated canonical pathways
Number of Focus Genes
Hepatic Fibrosis/Hepatic Stellate Cell Activation
Regulation of Actin-based Motility by Rho
Actin Cytoskeleton Signaling
Circadian Rhythm Signaling
One Carbon Pool by Folate
Biosynthesis of Steroids
Alanine and Aspartate Metabolism
Using the high-sensitivity Illumina microarray platform, we identified changes in the human SM cell transcriptome that were induced by tissue biopsy culture based on the explant technique. More than one thousand genes displayed differential expression between cultured myotubes and SM tissue. A similar number of genes were upregulated and downregulated: 65% and 50% of the genes in each group, respectively, were expressed in SM according to the IPA knowledge-base.
Ontology analysis of downregulated genes in SM cultures revealed the following. In terms of their associated cellular components, there was an over-representation of genes encoding proteins located in mitochondria. In terms of their associated cell process, the genes that were enriched were involved in energy metabolism and the muscle-system. In line with this, the genes that had the most reduced expression included those encoding key regulators of glycogenolysis (PYGM), fatty acid oxidation (CPT1B) and preservation of ATP formation (AMPD1). Various genes involved in glycolysis, energy consumption (UCP3) and oxidative phosphorylation (complexes I, II, III and IV) were also repressed. IPA pathway analysis consistently revealed that the majority of regulated metabolic genes are involved in mitochondrial pathways of energy production and the most regulated signaling pathway is mitochondrial dysfunction. The highly downregulated genes also included those that encode proteins associated with myofilaments, calcium-binding proteins and CAPN3, a muscle-specific calcium-activated nonlysosomal cysteine protease that contributes to sarcomere homeostasis . Downregulated genes are slow-type isoforms such as MYL3, MYBPC1 and MYOZ1 and isoforms that are predominantly expressed in fast-type muscle fibers such as TMOD4, CASQ1 and MYOZ3. Noteworthy, the lumbar paravertebral muscles in humans are described as containing fiber types, type I (slow) and type II (fast), in similar proportions [41, 42]. The downregulation found in the metabolic and muscle-system profile in SM cultures is consistent with findings in several myopathies. For instance, energy metabolism genes were the major group of genes downregulated in human muscle by disuse atrophy , DMD [6, 7], α-sarcoglycan deficiency  and X-linked myotubular myopathy (XLMTM) . Decreased expression of genes encoding muscle-system/contraction associated or regulatory proteins was observed in DMD  and XLMTM patients . In muscles of immobilized legs in patients , major categories of downregulated genes were involved in sarcomere structure and protein turnover (including the CAPN3 gene). According to our data, the full phenotype of several muscle pathologies may not be expressed in cultured muscle as the genes are markedly downregulated. This includes PYGM-deficiency (McArdle disease) ; lack of AMPD1, which causes metabolic myopathy in humans ; and loss-of-function mutations in the CAPN3 gene that have been associated with limb-girdle muscular dystrophy type 2A (LGMD2A) .
We identified potential regulators of SM function among the most downregulated genes. Myf6/MRF4 is a member of the family of myogenic regulatory factors that includes MyoD, Myf5 and myogenin . MRF4 expression is sufficient for conversion of fibroblasts to myogenic lineage . However, MRF4 did not stimulate myogenic differentiation in C2C12 cells, but cell proliferation, in contrast to MyoD . In addition, it was inefficient at trans-activating contractile protein genes, unlike MyoD and myogenin . Various genes encoding proteins that contain the ankyrin repeat structural motif were strongly downregulated, namely, the ASB family members ASB10 and ASB12. ASBs are implicated in various biological processes. For example, ASB15 promotes muscle cell growth and stimulates protein synthesis , while ASB6 regulates components of the insulin signaling pathway in adipocytes . Two members of the MARP family are also included: ANKRD2 and ANKRD23. ANKRD2/Arpp appears to be involved in sensing stress signals and linking these to muscle gene regulation , and ANKRD23/DARP is upregulated in insulin-resistant animals . Abrogation of the three MARP family members CARP, ANKRD2 and DARP in mice indicated a role in passive mechanical behavior and in the regenerative response in muscle .
In myotubes versus SM tissue, we found coordinated reduced expression of five members of the GIMAP gene family GIMAP1, GIMAP4, GIMAP5, GIMAP6 and GIMAP7, which form a cluster on chromosome 7 and participate in cell survival/death. Seven GIMAP genes are functional: GIMAP1, GIMAP2, GIMAP4, GIMAP5, GIMAP6, GIMAP7 and GIMAP8 . The GIMAPS are also called immune-associated nucleotide-binding proteins and are considered cell survival regulators, with a crucial role in lymphocytes: GIMAP5 is a key genetic factor for lymphopenia in spontaneous BioBreeding rat insulin-dependent diabetes and GIMAP4 has been shown to accelerate programmed cell death in T-cells . In humans, the GIMAP family genes are expressed in several tissues , although the highest expression of most of these genes is in immune tissues. However, to the best of our knowledge, this is the first description of coordinate regulation of the expression of the genes in this family, which indicates the participation of common mechanisms of transcriptional regulation: shared control regions or the same control elements in each gene.
In contrast, most of the upregulated genes in culture were located in the ER and extracellular matrix on the basis of the gene ontology analysis. Induced genes included those involved in tissue remodeling, such as genes encoding TIMP2, PLOD2 and PLOD3, CSGlcAT, SERPINE2 and MMP14, all of which were among the 10% most regulated. In addition, induced genes included those involved in the formation of the extracellular matrix, such as those encoding collagens, laminins, fibronectin, integrins and proteoglycans. This increase may be partially due to the enrichment of SM cultures in fibroblasts compared to the tissue. This is indicated by the increased expression, albeit less than that of the 10% most regulated, of the THY1 gene, which is expressed in fibroblasts, neurons and endothelial cells. Myotubes may also contribute to this increase in expression. In this regard, IPA analysis revealed alteration of signaling pathways related to cell interaction with the extracellular matrix/environment in either the whole set or the subset of SM-filtered differentially expressed genes. Remarkably, the proteoglycan SPOCK1/SPARC/OSTEONECTIN gene was highly induced. Upregulation of genes of the extracellular matrix are common findings in muscle from DMD [5–7], LGMD2A  and XLMTM patients , where it is considered to reflect dystrophic changes. In some cases, the SPARC gene is also included [5–7, 9]. In our SM cultures, the strong induction of DNAJC10/ERDJ5 gene expression suggests ER stress. DNAJC10/ERDJ5 is abundant in secretory cells and induced during ER stress . It resides in ER, where secreted proteins are translocated and encounter the folding machinery; it has disulfide reductase activity and is involved in the retro-translocation of misfolded proteins into cytosol for degradation .
One of the most induced genes in culture is that encoding CDKN1A/p21, an inhibitor of cell cycle progression, involved in the p53-dependent cellular senescence, possibly mediating apoptosis execution . Another highly induced gene encodes KIAA1199, a protein of undefined function that was identified as an induced transcript in replicative senescent-induced renal carcinoma cells by transfer of human chromosome 3 , and as a candidate for hearing loss . These data suggest that cultured muscle cells have activated a senescence process that was not established in the muscle tissue. Accordingly, the IPA analysis detected apoptosis as a regulated signaling pathway.
Among the most induced genes in SM cultures were potential transcriptional regulators. These included the gene encoding TOP2A, a DNA topoisomerase that is associated with the RNA polymerase II holoenzyme and is a required component of chromatin-dependent coactivation , and the HIF1A/MOP1 gene, which encodes the oxygen-sensitive alpha subunit of the transcription factor hypoxia inducible factor-1 (HIF-1) that forms a heterodimer with the beta subunit . During hypoxia the HIF1A protein is stabilized and activated. The HIF1A gene is expressed constitutively in some cell types, in others it is upregulated by hypoxia  or obesity , although downregulation of mRNA levels by hypoxia has also been reported in human fat cells . With respect to hypoxia, we found that the MB gene encoding the oxygen-binding myoglobin is one of the most repressed in cultured SM cells. Among the targets of HIF-1 , several genes showed increased expression in the SM cultures, such as the gene encoding the protein related to vascular tone, ADM; to angiogenesis, VEGFC; to glucose metabolism, ENO1; and the homolog of the pro-apoptotic BNIP3 protein BNIP3L/NIX gene, which suggests increased activity of the HIF-1 transcription factor. However, other HIF-1 gene targets involved in glucose metabolism  were not upregulated. The overexpression of HIF1A factor is related to local invasion and metastatic spread of tumor cells . Thus, in SM cultures it may contribute to the activation of the tissue remodeling process. Moreover, the HIF-1 factor has been related  to resistance to anoikis, a programmed cell death induced by the loss of or inappropriate cell adhesion, in transformed epithelial cells and it might also have this role in the SM cultures.
Two genes with potential involvement but no clear role in metabolic control also showed increased expression in culture. One is the gene encoding CCDC80/URB/SSG1/DRO1, which was identified as an upregulated transcript in the adipose tissue of bombesin receptor subtype-3 (BRS-3)-deficient mice displaying mild late-onset obesity  and sensitizes cells to anoikis and apoptosis . The other is the gene encoding LPIN2, which is a member of the lipin protein family that possesses phosphatidate phosphatase activity converting phosphatidate to diacylglycerol. The function of the latter has not yet been defined and mutations of the gene have been found in patients with Majeed syndrome, an auto-inflammatory disorder .
In summary, our data show a reduction in the metabolic and muscle-system transcriptome in the SM cultures that may be due to inappropriate stimuli, including the lack of innervation, the limited extracellular matrix molecules in SM cultures or other less-defined stimuli such as circadian rhythm. The fact that the tissue remodeling transcriptome is increased and signaling pathways related to cell interaction with the extracellular matrix/environment are regulated indicates that a lack of appropriate matrix stimuli may be involved, at least partly. The hypoxia response pathway, which appears to be activated in SM cultures, may to some extent trigger the tissue remodeling process. Finally, the atrophic phenotype of cultured myotubes is associated with the induction of genes related to apoptosis or anoikis and activation of the apoptosis signaling pathway.
We show that a high-sensitivity microarray platform enables analysis of SM tissue samples from individual donors and derived cultures at whole transcriptome level. Microarrays deliver the differential transcriptome. The main findings are that SM cultures show reductive metabolic and muscle-system transcriptome adaptations as observed in muscle atrophy. The metabolic reduction strongly affects key genes of the catabolism of glucose and lipids and moderately and extensively affects genes involved in mitochondrial energy production. In contrast, SM cultures show augmented tissue remodeling transcriptome and induction of genes involved in the apoptosis or anoikis process. Finally, the hypoxia-response pathway appears to participate in this adaptation. Biological pathway analysis by IPA provides insights into processes driven by the culturing of SM such as mitochondrial dysfunction and pathways related to cell interaction with the extracellular matrix and apoptosis. This study contributes to the definition of the phenotype of the SM primary culture, which is a valuable cell model that has key implications for the study of muscle pathogenesis and therapeutic assays.
We thank Anna Orozco (Bioquímica i Biologia Molecular, Universitat de Barcelona) for technical assistance. This work was supported by grants SAF2006-07228 from the Ministerio de Ciencia y Tecnología (MCyT), SAF2009-07559 from the Ministerio de Ciencia e Innovación and CIBER de Diabetes y Enfermedades Metabólicas Asociadas which is an ISCIII project, Spain. CGM was the recipient of a Ramon y Cajal fellowship from the Ministerio de Educación y Ciencia, Spain. We thank Enrique Blanco (Departament de Genètica, Facultat de Biologia, Universitat de Barcelona) for assistance with the bioinformatic analysis. We thank Manel Bosch and Nieves Hernández from the Unitat de Microscòpia Confocal of the Serveis Cientificotècnics (Facultat de Biologia, Universitat de Barcelona) for assistance with the immunocytochemistry analyses.
- Chen YW, Gregory CM, Scarborough MT, Shi R, Walter GA, Vandenborne K: Transcriptional pathways associated with skeletal muscle disuse atrophy in humans. Physiol Genomics. 2007, 31: 510-520. 10.1152/physiolgenomics.00115.2006.PubMedView Article
- Zhou X, Dimachkie MM, Xiong M, Tan FK, Arnett FC: cDNA microarrays reveal distinct gene expression clusters in idiopathic inflammatory myopathies. Med Sci Monit. 2004, 10: BR191-BR197.PubMed
- Greenberg SA: A gene expression approach to study perturbed pathways in myositis. Curr Opin Rheumatol. 2007, 19: 536-541. 10.1097/BOR.0b013e3282efe261.PubMedView Article
- Bakay M, Zhao P, Chen J, Hoffman EP: A web-accessible complete transcriptome of normal human and DMD muscle. Neuromuscul Disord. 2002, 12 (Suppl 1): S125-S141. 10.1016/S0960-8966(02)00093-7.PubMedView Article
- Haslett JN, Sanoudou D, Kho AT, Bennett RR, Greenberg SA, Kohane IS, Beggs AH, Kunkel LM: Gene expression comparison of biopsies from Duchenne muscular dystrophy (DMD) and normal skeletal muscle. Proc Natl Acad Sci USA. 2002, 99: 15000-15005. 10.1073/pnas.192571199.PubMed CentralPubMedView Article
- Noguchi S, Tsukahara T, Fujita M, Kurokawa R, Tachikawa M, Toda T, Tsujimoto A, Arahata K, Nishino I: cDNA microarray analysis of individual Duchenne muscular dystrophy patients. Hum Mol Genet. 2003, 12: 595-600. 10.1093/hmg/12.6.595.PubMedView Article
- Chen YW, Zhao P, Borup R, Hoffman EP: Expression profiling in the muscular dystrophies: identification of novel aspects of molecular pathophysiology. J Cell Biol. 2000, 151: 1321-1336. 10.1083/jcb.151.6.1321.PubMed CentralPubMedView Article
- Keira Y, Noguchi S, Kurokawa R, Fujita M, Minami N, Hayashi YK, Kato T, Nishino I: Characterization of lobulated fibers in limb girdle muscular dystrophy type 2A by gene expression profiling. Neurosci Res. 2007, 57: 513-521. 10.1016/j.neures.2006.12.010.PubMedView Article
- Noguchi S, Fujita M, Murayama K, Kurokawa R, Nishino I: Gene expression analyses in X-linked myotubular myopathy. Neurology. 2005, 65: 732-737. 10.1212/01.wnl.0000174625.67484.4d.PubMedView Article
- Nygaard V, Hovig E: Options available for profiling small samples: a review of sample amplification technology when combined with microarray profiling. Nucleic Acids Res. 2006, 34: 996-1014. 10.1093/nar/gkj499.PubMed CentralPubMedView Article
- Askanas V, Gallez-Hawkins G: Synergistic influence of polypeptide growth factors on cultured human muscle. Arch Neurol. 1985, 42: 749-752.PubMedView Article
- Zammit PS, Partridge TA, Yablonka-Reuveni Z: The skeletal muscle satellite cell: the stem cell that came in from the cold. J Histochem Cytochem. 2006, 54: 1177-1191. 10.1369/jhc.6R6995.2006.PubMedView Article
- Bonavaud S, Agbulut O, Nizard R, D'honneur G, Mouly V, Butler-Browne G: A discrepancy resolved: human satellite cells are not preprogrammed to fast and slow lineages. Neuromuscul Disord. 2001, 11: 747-752. 10.1016/S0960-8966(01)00222-X.PubMedView Article
- Mouly V, Edom F, Barbet JP, Butler-Browne GS: Plasticity of human satellite cells. Neuromuscul Disord. 1993, 3: 371-377. 10.1016/0960-8966(93)90080-4.PubMedView Article
- lannaccone ST, Nagy B, Samaha FJ: Partial biochemical maturation of aneurally cultured human skeletal muscle. Neurology. 1982, 32: 846-851.View Article
- Martinuzzi A, Askanas V, Kobayashi T, Engel WK, Di Mauro S: Expression of muscle-gene-specific isozymes of phosphorylase and creatine kinase in innervated cultured human muscle. J Cell Biol. 1986, 103: 1423-1429. 10.1083/jcb.103.4.1423.PubMedView Article
- Ferrer-Martínez A, Montell E, Montori-Grau M, García-Martínez C, Gómez-Foix AM, Roberts MA, Mansourian R, Macé K: Long-term cultured human myotubes decrease contractile gene expression and regulate apoptosis-related genes. Gene. 2006, 384: 145-153. 10.1016/j.gene.2006.07.042.PubMedView Article
- Van Gelder RN, von Zastrow ME, Yool A, Dement WC, Barchas JD, Eberwine JH: Amplified RNA synthesized from limited quantities of heterogeneous cDNA. Proc Natl Acad Sci USA. 1990, 87: 1663-1667. 10.1073/pnas.87.5.1663.PubMed CentralPubMedView Article
- Raymond F, Metairon S, Borner R, Hofmann M, Kussmann M: Automated target preparation for microarray-based gene expression analysis. Anal Chem. 2006, 78: 6299-6305. 10.1021/ac060097t.PubMedView Article
- Verdugo RA, Deschepper CF, Muñoz G, Pomp D, Churchill GA: Importance of randomization in microarray experimental designs with Illumina platforms. Nucleic Acids Res. 2009, 37: 5610-5618. 10.1093/nar/gkp573.PubMed CentralPubMedView Article
- Canales RD, Luo Y, Willey JC, Austermiller B, Barbacioru CC, Boysen C, Hunkapiller K, Jensen RV, Knight CR, Lee KY, Ma Y, Maqsodi B, Papallo A, Peters EH, Poulter K, Ruppel PL, Samaha RR, Shi L, Yang W, Zhang L, Goodsaid FM: Evaluation of DNA microarray results with quantitative gene expression platforms. Nature Biotechnology. 2006, 24: 1115-1122. 10.1038/nbt1236.PubMedView Article
- Coppe A, Danieli GA, Bortoluzzi S: REEF: searching REgionally Enriched Features in genomes. BMC Bioinformatics. 2006, 7: 453-10.1186/1471-2105-7-453.PubMed CentralPubMedView Article
- Paulin D, Li Z: Desmin: a major intermediate filament protein essential for the structural integrity and function of muscle. Exp Cell Res. 2004, 301: 1-7. 10.1016/j.yexcr.2004.08.004.PubMedView Article
- Su AI, Wiltshire T, Batalov S, Lapp H, Ching KA, Block D, Zhang J, Soden R, Hayakawa M, Kreiman G, Cooke MP, Walker JR, Hogenesch JB: A gene atlas of the mouse and human protein-encoding transcriptomes. Proc Natl Acad Sci USA. 2004, 101: 6062-6067. 10.1073/pnas.0400782101.PubMed CentralPubMedView Article
- Hardison R: Hemoglobins from bacteria to man: evolution of different patterns of gene expression. J Exp Biol. 1998, 201: 1099-1117.PubMed
- Rege TA, Hagood JS: Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis. FASEB J. 2006, 20: 1045-1054. 10.1096/fj.05-5460rev.PubMedView Article
- Zatz M, Starling A: Calpains and disease. N Engl J Med. 2005, 352: 2413-2423. 10.1056/NEJMra043361.PubMedView Article
- Myllylä R, Wang C, Heikkinen J, Juffer A, Lampela O, Risteli M, Ruotsalainen H, Salo A, Sipilä L: Expanding the lysyl hydroxylase toolbox: new insights into the localization and activities of lysyl hydroxylase 3 (LH3). J Cell Physiol. 2007, 212: 323-329. 10.1002/jcp.21036.PubMedView Article
- Gotoh M, Yada T, Sato T, Akashima T, Iwasaki H, Mochizuki H, Inaba N, Togayachi A, Kudo T, Watanabe H, Kimata K, Narimatsu H: Molecular cloning and characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine. J Biol Chem. 2002, 277: 38179-38188. 10.1074/jbc.M202601200.PubMedView Article
- Stetler-Stevenson WG, Krutzsch HC, Liotta LA: Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family. J Biol Chem. 1989, 264: 17374-17378.PubMed
- Carter RE, Cerosaletti KM, Burkin DJ, Fournier RE, Jones C, Greenberg BD, Citron BA, Festoff BW: The gene for the serpin thrombin inhibitor (PI7), protease nexin I, is located on human chromosome 2q33-q35 and on syntenic regions in the mouse and sheep genomes. Genomics. 1995, 27: 196-199. 10.1006/geno.1995.1025.PubMedView Article
- Ango F, di Cristo G, Higashiyama H, Bennett V, Wu P, Huang ZJ: Ankyrin-based subcellular gradient of neurofascin, an immunoglobulin family protein, directs GABAergic innervation at Purkinje axon initial segment. Cell. 2004, 119: 257-272. 10.1016/j.cell.2004.10.004.PubMedView Article
- Margis R, Dunand C, Teixeira FK, Margis-Pinheiro M: Glutathione peroxidase family - an evolutionary overview. FEBS J. 2008, 275: 3959-3970. 10.1111/j.1742-4658.2008.06542.x.PubMedView Article
- Bottazzi B, Garlanda C, Cotena A, Moalli F, Jaillon S, Deban L, Mantovani A: The long pentraxin PTX3 as a prototypic humoral pattern recognition receptor: interplay with cellular innate immunity. Immunol Rev. 2009, 227: 9-18. 10.1111/j.1600-065X.2008.00719.x.PubMedView Article
- Beutler E: Gaucher disease: new molecular approaches to diagnosis and treatment. Science. 1992, 256: 794-799. 10.1126/science.1589760.PubMedView Article
- Huitema K, Dikkenberg van den J, Brouwers JFHM, Holthuis JCM: Identification of a family of animal sphingomyelin synthases. EMBO J. 2004, 23: 33-44. 10.1038/sj.emboj.7600034.PubMed CentralPubMedView Article
- Dalgaard LT, Pedersen O: Uncoupling proteins: functional characteristics and role in the pathogenesis of obesity and Type II diabetes. Diabetologia. 2001, 44: 946-965. 10.1007/s001250100596.PubMedView Article
- Ruderman NB, Saha AK, Vavvas D, Witters LA: Malonyl-CoA, fuel sensing, and insulin resistance. Am J Physiol. 1999, 276: E1-E18.PubMed
- Lenders JW, Eisenhofer G, Abeling NG, Berger W, Murphy DL, Konings CH, Wagemakers LM, Kopin IJ, Karoum F, van Gennip AH, Brunner HG: Specific genetic deficiencies of the A and B isoenzymes of monoamine oxidase are characterized by distinct neurochemical and clinical phenotypes. J Clin Invest. 1996, 97: 1010-1019. 10.1172/JCI118492.PubMed CentralPubMedView Article
- Keniry M, Parsons R: The role of PTEN signaling perturbations in cancer and in targeted therapy. Oncogene. 2008, 27: 5477-5485. 10.1038/onc.2008.248.PubMedView Article
- Sirca A, Kostevc V: The fibre type composition of thoracic and lumbar paravertebral muscles in man. J Anat. 1985, 141: 131-137.PubMed CentralPubMed
- Thorstensson A, Carlson H: Fibre types in human lumbar back muscles. Acta Physiol Scand. 1987, 131: 195-202. 10.1111/j.1748-1716.1987.tb08226.x.PubMedView Article
- Lucia A, Nogales-Gadea G, Pérez M, Martín MA, Andreu AL, Arenas J: McArdle disease: what do neurologists need to know?. Nat Clin Pract Neurol. 2008, 4: 568-577. 10.1038/ncpneuro0913.PubMedView Article
- Gross M: Clinical heterogeneity and molecular mechanisms in inborn muscle AMP deaminase deficiency. J Inherit Metab Dis. 1997, 20: 186-192. 10.1023/A:1005352605421.PubMedView Article
- Pownall ME, Gustafsson MK, Emerson CP: Myogenic regulatory factors and the specification of muscle progenitors in vertebrate embryos. Annu Rev Cell Dev Biol. 2002, 18: 747-783. 10.1146/annurev.cellbio.18.012502.105758.PubMedView Article
- Yutzey KE, Rhodes SJ, Konieczny SF: Differential trans activation associated with the muscle regulatory factors MyoD1, myogenin, and MRF4. Mol Cell Biol. 1990, 10: 3934-3944.PubMed CentralPubMedView Article
- Jin X, Kim JG, Oh MJ, Oh HY, Sohn YW, Pian X, Yin JL, Beck S, Lee N, Son J, Kim H, Yan C, Wang JH, Choi YJ, Whang KY: Opposite roles of MRF4 and MyoD in cell proliferation and myogenic differentiation. Biochem Biophys Res Commun. 2007, 364: 476-482. 10.1016/j.bbrc.2007.10.042.PubMedView Article
- McDaneld TG, Hannon K, Moody DE: Ankyrin repeat and SOCS box protein 15 regulates protein synthesis in skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2006, 290: R1672-R1682.PubMedView Article
- Wilcox A, Katsanakis KD, Bheda F, Pillay TS: Asb6, an adipocyte-specific ankyrin and SOCS box protein, interacts with APS to enable recruitment of elongins B and C to the insulin receptor signaling complex. J Biol Chem. 2004, 279: 38881-38888. 10.1074/jbc.M406101200.PubMedView Article
- Kojic S, Medeot E, Guccione E, Krmac H, Zara I, Martinelli V, Valle G, Faulkner G: The Ankrd2 protein, a link between the sarcomere and the nucleus in skeletal muscle. J Mol Biol. 2004, 339: 313-325. 10.1016/j.jmb.2004.03.071.PubMedView Article
- Ikeda K, Emoto N, Matsuo M, Yokoyama M: Molecular identification and characterization of a novel nuclear protein whose expression is up-regulated in insulin-resistant animals. J Biol Chem. 2003, 278: 3514-3520. 10.1074/jbc.M204563200.PubMedView Article
- Barash IA, Bang ML, Mathew L, Greaser ML, Chen J, Lieber RL: Structural and regulatory roles of muscle ankyrin repeat protein family in skeletal muscle. Am J Physiol Cell Physiol. 2007, 293: C218-C227. 10.1152/ajpcell.00055.2007.PubMedView Article
- Krücken J, Schroetel RM, Müller IU, Saïdani N, Marinovski P, Benten WP, Stamm O, Wunderlich F: Comparative analysis of the human gimap gene cluster encoding a novel GTPase family. Gene. 2004, 341: 291-304. 10.1016/j.gene.2004.07.005.PubMedView Article
- Nitta T, Takahama Y: The lymphocyte guard-IANs: regulation of lymphocyte survival by IAN/GIMAP family proteins. Trends Immunol. 2007, 28: 58-65. 10.1016/j.it.2006.12.002.PubMedView Article
- Cunnea PM, Miranda-Vizuete A, Bertoli G, Simmen T, Damdimopoulos AE, Hermann S, Leinonen S, Huikko MP, Gustafsson JA, Sitia R, Spyrou G: ERdj5, an endoplasmic reticulum (ER)-resident protein containing DnaJ and thioredoxin domains, is expressed in secretory cells or following ER stress. J Biol Chem. 2003, 278: 1059-1066. 10.1074/jbc.M206995200.PubMedView Article
- Ushioda R, Hoseki J, Araki K, Jansen G, Thomas DY, Nagata K: ERdj5 is required as a disulfide reductase for degradation of misfolded proteins in the ER. Science. 2008, 321: 569-572. 10.1126/science.1159293.PubMedView Article
- Zhang H: Molecular signaling and genetic pathways of senescence: Its role in tumorigenesis and aging. J Cell Physiol. 2007, 210: 567-574. 10.1002/jcp.20919.PubMedView Article
- Michishita E, Garcés G, Barrett JC, Horikawa I: Upregulation of the KIAA1199 gene is associated with cellular mortality. Cancer Lett. 2006, 239: 71-77. 10.1016/j.canlet.2005.07.028.PubMedView Article
- Abe S, Usami S, Nakamura Y: Mutations in the gene encoding KIAA1199 protein, an inner-ear protein expressed in Deiters' cells and the fibrocytes, as the cause of non-syndromic hearing loss. J Hum Genet. 2003, 48: 564-570. 10.1007/s10038-003-0079-2.PubMedView Article
- Mondal N, Parvin JD: DNA topoisomerase II-alpha is required for RNA polymerase II transcription on chromatin templates. Nature. 2001, 413: 435-438. 10.1038/35096590.PubMedView Article
- Ke Q, Costa M: Hypoxia-inducible factor-1 (HIF-1). Mol Pharmacol. 2006, 70: 1469-1480. 10.1124/mol.106.027029.PubMedView Article
- Wiener CM, Booth G, Semenza GL: In vivo expression of mRNAs encoding hypoxia-inducible factor 1. Biochem Biophys Res Commun. 1996, 225: 485-488. 10.1006/bbrc.1996.1199.PubMedView Article
- Cancello R, Henegar C, Viguerie N, Taleb S, Poitou C, Rouault C, Coupaye M, Pelloux V, Hugol D, Bouillot JL, Bouloumié A, Barbatelli G, Cinti S, Svensson PA, Barsh GS, Zucker JD, Basdevant A, Langin D, Clément K: Reduction of macrophage infiltration and chemoattractant gene expression changes in white adipose tissue of morbidly obese subjects after surgery-induced weight loss. Diabetes. 2005, 54: 2277-2286. 10.2337/diabetes.54.8.2277.PubMedView Article
- Wang B, Wood IS, Trayhurn P: Dysregulation of the expression and secretion of inflammation-related adipokines by hypoxia in human adipocytes. Pflügers Archiv Eur J Physiol. 2007, 455: 479-492. 10.1007/s00424-007-0301-8.View Article
- Rohwer N, Welzel M, Daskalow K, Pfander D, Wiedenmann B, Detjen K, Cramer T: Hypoxia-inducible factor 1alpha mediates anoikis resistance via suppression of alpha5 integrin. Cancer Res. 2008, 68: 10113-10120. 10.1158/0008-5472.CAN-08-1839.PubMedView Article
- Aoki K, Sun YJ, Aoki S, Wada K, Wada E: Cloning, expression, and mapping of a gene that is upregulated in adipose tissue of mice deficient in bombesin receptor subtype-3. Biochem Biophys Res Commun. 2002, 290: 1282-1288. 10.1006/bbrc.2002.6337.PubMedView Article
- Bommer GT, Jäger C, Dürr EM, Baehs S, Eichhorst ST, Brabletz T, Hu G, Fröhlich T, Arnold G, Kress DC, Göke B, Fearon ER, Kolligs FT: DRO1, a gene down-regulated by oncogenes, mediates growth inhibition in colon and pancreatic cancer cells. J Biol Chem. 2005, 280: 7962-7975. 10.1074/jbc.M412593200.PubMedView Article
- Al-Mosawi ZS, Al-Saad KK, Ijadi-Maghsoodi R, El-Shanti HI, Ferguson PJ: A splice site mutation confirms the role of LPIN2 in Majeed syndrome. Arthritis Rheum. 2007, 56: 960-964. 10.1002/art.22431.PubMedView Article
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.