Figure 1

MiRNome analysis of childhood CNS GCTs. (A) Overall survival rates of GCTs of different histological subtypes. In total, 161 patients were followed up for up to 20 years. These were then subjected to Kaplan-Meier survival analysis. Numbers in parentheses are case numbers of each tumor subtype. Vertical lines indicate the censored survival observations. (B) Principal component analysis (PCA) using the filtered miRNAs (p < 0.05 and fold change ≧2). Each spot represents a single array. (C) A heat map shows the miRNAs enriched in the different prognostic groups. MiRNAs in red showed increased expression, while those in blue showed decreased. (D-E) Validation of miRNA array results by real-time PCR. The mean expression levels of the target miRNAs are compared to that of the U6 small nuclear RNA control. Results are expressed as the mean ± standard deviation (SD) (E). The miRNAs' array hybridization signals are also shown (D).