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Table 2 Characteristics of the sequenced loci and primers used for PCR.

From: Genome dynamics of Bartonella grahamii in micro-populations of woodland rodents

No.

Locusa

Positionb and name or sequence (5'-3') of primers

Size (bp)c

No. of allelesd

1

Bgr_05150 (cycK)

622901 GCGCTGCTTACTTTTTCCC

580

1

  

623480 TCTTTCCCCATAGATCCGC

  

2

Bgr_00610 (batR)

82636 CAATGGTGCGATCATCTACG

535

1

  

83190 CGTCTTTATCTTTTGCGCTTG

  

3

Bgr_07230 (gltA)

872963 BhCS.1137n [61]

1039

1

  

874001 CS140f [60]

  

4

Bgr_13910 (ftsZ)

1557200 Bh ftsZ 1754.n [66]

817

2 (1)

  

1558016 Bh ftsZ 965.p [66]

  

5

Bgr_00430

61980 ATGCACAGCTTTCTGGTCG

590

1

  

62569 TCCTGCAATAAAACCATTTGC

  

6

Bgr_02350 (phoH)

334448 ATCAAAACAACTTGGCTCGG

692 (158)

1

 

Bgr_02360

335139 TTCAGGCGATTTCATTGTAGG

  

7

Bgr_03900 (aldA)

473807 TGTTTTCCATTTTTGAAACGC

725 (469)

2 (1)

 

Bgr_03910 (ftsK1)

474531 CTTCTCTTGATGCACCTTTCG

  

8

Bgr_05600 (cspA)

679952 TGAATCCGAAACCTTTTGTTG

617 (245)

2 (1)

 

Bgr_05610 (carB)

680568 TTGGCTTTTCTGTTGTCGC

  

9

Bgr_06060 (pssA)

734202 TAGGCGCTCTTGGTTTGG

727 (70)

1

 

Bgr_06070

734928 TGGACGAGCCATTCTGTTATC

  

10

Bgr_06380 (uvrC)

776004 CAATCATCCGGTAAACCCC

768 (397)

1

 

Bgr_06390

776771 TGAAATGCGTATCCGAAAAAG

  

11

Bgr_11560 (maeB2)

1305872 TTTTCGTGATCGTGTTTTTCC

723 (189)

1

 

Bgr_11570 (acpP2)

1306594 GCCTGTTTTAAGGCAACGAG

  

12

Bgr_15900 (asd)

1810101 CTCCGCGATGCTCCC

666 (281)

1

 

Bgr_15910

1810766 AAATCCTTCGCCCAAAGC

  

13

Bgr_17890

2061896 CAACATTAGGGGGATTGGG

592 (245)

1

 

Bgr_17900 (dut)

2062487 AGCCGTTGCGTAGTGAGG

  

14

Bgr_18510 (pgk)

2129539 ACCCCATCACTGCTTCCTC

605 (514)

1

 

Bgr_18520 (gap)

2130143 CGCGTTTTGGTTTGGTATG

  

15

Bgr_19030 (ftsK2)

2203018 CAATAAGACGCGAACCTTGAG

880 (723)

2 (2)

 

Bgr_19040

2203897 TCCCCCTGCAATGAGAAG

  

16

Bgr_19460 (dnaJ2)

2239347 GCAAAGATTCGCTCTGGAAC

708 (201)

1

 

Bgr_19470 (cobS)

2240054 ATAGCCAGAAACCATCACACG

  

17

Bgr_19730

2273643 CAAGGATTTCGTGCCCC

811 (144)

1

 

Bgr_19740

2274453 TTATGTTTCGCGGTTGTTCTC

  
  1. a The locus_tag and gene name, if any, for the sequenced regions in B. grahamii as4aup. For intergenic regions, both the upstream and the downstream genes are shown.
  2. bGenomic positions of primers refer to the B. grahamii as4aup genome.
  3. c Size of the total PCR product refer to the B. grahamii as4aup genome. Within parenthesis is the size of the spacer region, if any.
  4. dWithin parenthesis is the number of variable sites.
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BMC Genomics

ISSN: 1471-2164

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