Strain or plasmid | Relevant characteristicsa | Reference or source |
---|---|---|
Escherichia coli | ||
DH5α | supE 44, ΔlacU 169, f 80, lacZ ΔM, recA 1, endA 1, gyrA 96, thi 1, relA 1, 5hsdR 171 | Bethesda Research Lab® |
S17.1 | thi, pro, recA, hsdR, hsdM, Rp4Tc::Mu, Km::Tn7; Tpr, Smr, Spr | [57] |
Sinorhizobium meliloti | ||
GR4 | Wild type | [58] |
QS77 | GR4 (fadD::Tn5), Kmr | [16] |
Rm1021 | SU47 expR102::ISRm 2011-1, Smr | [59] |
1021FDC5 | Rm1021 (ΔfadD::Km), Smr Kmr | This work |
1021FDCSS | Rm1021 (ΔfadD::SmSp), Smr Spr | This work |
Rm2011 | SU47expR102::ISRm 2011-1, Smr | [60] |
2011FDC | Rm2011 (ΔfadD::SmSp), Smr Spr | This work |
SmA818 | Rm2011 pSymA cured, Smr | [61] |
A818FDC | SmA818 (ΔfadD::SmSp), Smr Spr | This work |
2011rhbA62 | Rm2011 (rhbA:: Tn5lac), Smr Rifr Nmr | [30] |
2011rhbAFDC | 2011rhbA62 (ΔfadD::SmSp), Smr Spr Rifr Nmr | This work |
2011rhbE11 | Rm2011 (rhbE:: Tn5lac), Smr Rifr Nmr | [30] |
2011rhbEFDC | 2011rhbE11 (ΔfadD::SmSp), Smr Spr Rifr Nmr | This work |
2011rhrA26 | Rm2011 (rhrA:: Tn5lac), Smr Rifr Nmr | [30] |
2011rhrAFDC | 2011rhrA26 (ΔfadD::SmSp), Smr Spr Rifr Nmr | This work |
2011rhtA1 | Rm2011 (rhtA:: Tn5), Smr Rifr Nmr | [30] |
2011rhtAFDC | 2011rhtA1 (ΔfadD::SmSp), Smr Spr Rifr Nmr | This work |
G212rirA | Rm1021 (lac-, rirA::Km), Smr Kmr | O'Connell, M. |
G212rirAFDC | G212rirA (ΔfadD::SmSp), Smr Kmr | This work |
Plasmids | ||
pBSKS(+) | Cloning vector; Apr | Stratagene |
pHP45Ω | Plasmid containing Sm/Sp cassette; Apr, Smr, Spr | [62] |
pHP45Ω Km | Plasmid containing Km cassette; Apr, Kmr | [63] |
pK18mobsacB | Suicide plasmid; Kmr | [51] |
pBBRD4 | pBBR1 MCS-3 derivative containing the fadD gene of S. meliloti GR4; Tcr | [16] |
pBSDIL12 | pBSKS derivative containing the fadD gene of S. meliloti GR4; Apr | This work |
pBS12.6Km | pBSDIL12 in which the fadD gene has been deleted and interrupted with a Km cassette; Apr Kmr | This work |
pK18fadDCKm | pK18mobsacB carrying the fadD mutated version of pBS12.6Km; | This work |
pK18fadDCSS | pK18fadDCKm in which the Km cassette interrupting the fadD gene has been substituted by a Sm/Sp cassette | This work |