Figure 1

Effect of ethanol fixation on RNA quality and quantity. A) RNA quality (RIN scores) of the tissue sections in the presence (n = 24) and absence (n = 24) of ethanol fixation; B) RNA quantity (ng) of the tissue sections in the presence (n = 24) and absence (n = 24) of ethanol fixation; C) RIN scores of the tissue sections over four time points in the presence (n = 6 per time point) and absence (n = 6 per time point) of ethanol fixation and D) RNA quantity (ng) of the tissue sections over four time points in the presence (n = 6 per time point) and absence (n = 6 per time point) of ethanol fixation. Error bars indicate the corresponding SD. The large errors of the experiments were due to the fact that each tested group consisted of three different tissue types (normal, adenoma and carcinoma) which had the different RNA quality and quantity.