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Figure 5 | BMC Genomics

Figure 5

From: Depletion of the non-coding regulatory 6S RNA in E. coli causes a surprising reduction in the expression of the translation machinery

Figure 5

During stationary growth the basal ppGpp level is increased in 6S RNA deficient strains. NTPs extracted after in vivo labelling from two independent experiments were separated by thin layer chromatography. Lanes 1 and 3: extracts from ssrS- strain, lanes 2 and 4: extracts from the wild-type MC4100, lanes 5 and 6: extract from the relA+ strain MG1655. The sample in lane 6 had been treated with serine hydroxamate to induce the stringent control in order to produce high levels of ppGpp and pppGpp, which served as mobility markers.

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BMC Genomics

ISSN: 1471-2164

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