Skip to main content

Table 2 Genes assayed by QPCR in ovarian samples from Oncorhynchus mykiss and from Xenopus laevis

From: Comparative transcriptomic analysis of follicle-enclosed oocyte maturational and developmental competence acquisition in two non-mammalian vertebrates

 

-------------------Oncorhynchus mykiss-----------------

----------------------Xenopus laevis-------------------

target genes

symbol

GenBank#

LV/PV

LV/MAT

PV/MAT

LV/PV

LV/MAT

PV/MAT

symbol

GenBank#

IV/VI

IV/MAT

VI/MAT

   

------microarray ratio----

--------QPCR ratio--------

  

--------QPCR ratio--------

*Aromatase

cyp19a1a

BX083177

2.58

25

9.67

2.6

182

76

P450arom-A

NM_001085653

313

1237

3.95

*Cytochrome P450 17A1

cyp17a1

BX072662

0.84

2.46

2.92

1.15

4.7

4.5

cyp17a1

AF325435

2.41

3.77

1.56

* RNA-binding region-containing protein 39

rbm39a

BX081888

1.77

3.06

1.73

0.59

0.52

0.99

rbm39

BC077813

1.68

2.25

1.34

* A disintegrin and metalloproteinase domain 8

adam8a

BX871415

0.50

0.23

0.45

1.18

0.10

0.18

     

A disintegrin and metalloproteinase domain 13

        

adam13

NM_001087445

2.28

2.94

1.29

* A disintegrin and metalloproteinase domain 22

adam22

CA363158

1.00

0.42

0.42

0.47

0.14

0.30

     

A disintegrin and metalloproteinase domain 11

        

mdc11b

NM_001087444

1.41

1.65

1.17

* Protein kinase C delta type

prkcd

CA372310

1.02

0.52

0.51

0.77

0.09

0.12

pkc-delta2

NM_001090993

1.43

2.19

1.52

* Serum/glucocorticoid related kinase 2

sgk2

CA387850

0.99

0.24

0.24

0.01

0.0007

0.08

sgk1a

NM_001090340

2.97

1.72

0.58

* Serine protease 23

prss23

BX087643

0.53

0.26

0.49

0.21

0.07

0.35

prss23

CD255705

2.79

7.85

2.82

* Regulator of G-protein signaling 18

rgs18

BX876662

1.04

0.42

0.40

0.94

0.98

1.18

rgs18

NM_001092321

1.95

2.17

1.11

* Forkhead box O5

foxo5

BX885992

0.91

0.32

0.35

0.55

0.10

0.19

     

Forkhead box O3

        

foxo3

NM_001092949

1.94

1.85

0.95

* Cytidine monophosphate-N-acetylneuraminic acid hydroxylase

cmah

BX878414

1.28

5.88

4.58

1.26

10.57

9.26

cmah

NM_001086828

7.26

6.16

0.85

*Steroidogenic acute regulatory protein

star

BX079021

0.81

0.34

0.41

0.56

0.20

0.35

star-A

AF220437

0.23

0.02

0.10

* Growth arrest and DNA-damage-inducible protein beta

gadd45bii

CA363171

0.83

0.36

0.43

0.59

0.14

0.26

gadd45b

DT070441

2.41

2.89

1.20

*Apolipoprotein CI

apoc1

CA353171

0.27

0.14

0.53

0.26

0.13

0.52

apoc1

DQ096911

0.23

0.40

1.75

*11-beta 3-hydroxysteroid dehydrogenase

hsd11b3

CA348069

0.83

1.2

1.45

1.29

3.69

2.97

hsd11b3

BC106472

2.18

3.09

1.41

* Receptor-type tyrosine-protein phosphatase F

ptprf

CA360891

0.68

0.38

0.56

0.69

0.28

0.43

xptp-d

NM_001090381

2.71

8.97

3.31

* Dystrophin

dmd

CA377239

0.83

0.11

0.13

0.53

0.55

0.84

dmd

X99700

2.11

2.38

1.13

* Cyclin L1

ccnl1

BX863114

0.79

0.55

0.70

1.01

0.65

0.64

ccnl1

BC073707

1.40

1.66

1.19

*Transferrin receptor protein 1

tfr1

BX860777

1.36

3.07

2.26

1.47

3.41

2.50

tfr1

CA790915

1.98

7.12

3.58

* C-ETS-2 protein

ets2

CA368141

0.99

0.39

0.39

1.11

0.36

0.34

ets2b

X52635

1.40

1.96

1.40

* ETS translocation variant 5

etv5

BX870637

0.98

0.71

0.74

0.59

0.62

1.18

     

XER81

        

xer81

AF057565

1.38

1.69

1.22

* Gap junction Connexin-32.2 protein

cx32.2

BX082081

0.96

1.2

1.25

0.17

0.13

0.82

cx32

DQ096928

1.28

2.45

1.91

* Tight junction protein ZO-1

tjp1

BX872029

0.87

0.48

0.55

0.85

0.45

0.54

tjp1

BC088825

2.36

2.84

1.21

* Claudin-11

cldn11

BX301535

0.77

0.37

0.48

0.43

0.16

0.39

cldn11

CB591807.1

1.90

2.66

1.40

*Tescalcin

tesc

BX877446

0.71

2.34

3.27

0.84

13.19

13.90

tesc

NM_001095029

2.44

2.34

0.96

* 7-dehydrocholesterol reductase

dhcr7

BX884545

0.87

0.48

0.55

1.17

0.26

0.25

dhcr7

NM_001087087

1.96

1.79

0.91

  1. For each gene, official name, official symbol, GenBank accession number, and expression ratio among studied stages deduced from microarray and QPCR analyses are indicated. Target gene names are bolded when their expression profile during late oogenesis is similar in both species. Thirteen rainbow trout females were used for the transcriptomic analysis (FV, n = 3; PV, n = 4; females; Mat., n = 6) [14], while 28 females (FV, n = 6; PV, n = 14 females; Mat., n = 8) were used for QPCR, including the females used for the transcriptomic analysis.
Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us