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Table 2 Oligonucleotide sequences.

From: Complementary transcriptomic, lipidomic, and targeted functional genetic analyses in cultured Drosophila cells highlight the role of glycerophospholipid metabolism in Flock House virus RNA replication

Name/description

Sequence (5' → 3')

Cct1-for

*GAGATGGCCGAGAAGTTGAG

Cct1-rev

*GGATTCCCTCGATTCTCACA

Cct2-for

*ACTTGGGCCTGAGACTGCTA

Cct2-rev

*CTTTCGGCATTCTGTCCATT

Act5C-for

ATGTGTGACGAAGAAGTTGCT

Act5C-rev

GTCCCAGTTGGTCACGATACC

FHV1-RNAi-for

TAATACGACTCACTATAGGG ATGACTCTAAAAGTTATTC

FHV1-RNAi-rev

TAATACGACTCACTATAGGG TCTGCTAGCGATAAAC

S2-RNAi-T7

TAATACGACTCACTATAGGG AGACCACGGGCGGGT

  1. The T7 RNA polymerase promoter is in bold italics type for FHV1 RNAi-for, FHV1 RNAi-rev, and S2-RNAi-T7 oligonucleotides, and was also added to the 5' end of the Cct1 and Cct2 oligonucleotides (asterisks) that were used to generate dsRNAs.
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BMC Genomics

ISSN: 1471-2164

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