Differential expression of genes in salivary glands of male Rhipicephalus (Boophilus)microplus in response to infection with Anaplasma marginale

Table 2 A. marginale infection levels in cultured BME26 tick cells after RNAi.

Experimental group	Silencing of gene expression (%) ± SD^a	*A. marginale* infection levels (% change with respect to controls)^b
59Rib	71.7 ± 31.1	8.6 ± 0.5 (-3%)
93 Meth	65.0 ± 14.4	18.0 ± 0.0 (+102%)*
100Silk	99.5 ± 0.3	7.8 ± 0.6 (-12%)*
Subolesin (4D8)	88.1 ± 7.1	7.4 ± 0.3 (-17%)*
Control	--	8.9 ± 1.4

^aTotal RNA was extracted from infected tick cells after RNAi and analyzed by real-time RT-PCR to determine gene expression silencing with respect to control cells treated with buffer only.
^bThe A. marginale infection levels were analyzed by msp4 PCR, expressed as msp4 DNA (ng) ± SD and compared between test and control ticks by Student's t test (*p < 0.05).

ISSN: 1471-2164