Figure 5

Genome-wide re-distribution of Gcn5 when cells are exposed to KCl stress. Average gene analysis shows the average gene distribution of Gcn5-myc enrichment in the absence (left panel) and presence of KCl (right panel). (A) Average Gcn5-myc localisation is shown for groups of up-regulated, down-regulated and unchanged genes. (B) Gcn5-myc is preferentially enriched in highly expressed genes in the absence and presence of KCl stress. Genes are grouped according to their expression level as described in Methods section. (C) Gcn5-myc is preferentially enriched on long genes under KCl stress but is under-represented on such genes in the absence of stress. Genes are grouped according to their ORF length. (D) Gcn5 redistributes between groups of genes with different Gcn5-binding patterns in response to KCl stress. The graphs show the average distribution of Gcn5 in 5 different K-means clusters that were selected to reveal gene groups with different patterns of Gcn5 localisation throughout the genes. The shift of Gcn5 association between groups in response to KCl stress is shown.