Table 1 Primer pairs used for QPCR experiments
Gene symbol | Forward sequence | Reverse Sequence | Optimal primer conc F/R (fmol) |
|---|---|---|---|
3' Actin | GCTCTGGCTCCTAGCACCAT | AGCCACCGATCCACACAGAGT | 300/300 |
HspB8 | GGGCCTGCTCATCATCGA | GAGGAAGCTCGTTGTTGAAGCT | 300/300 |
Nme1 | CGCAGAACTGGATCTATGAGTGA | CCCCTGCCTGTGAGAACAA | 300/300 |
Zfp101 | GGATGAAATCCTGTTCCCATACAT | TGTCCTGGATTAGATACTGTATTTTGATA | 900/900 |
Fos | ATTGTCGAGGTGGTCTGAATGTT | AACGTTTTCATGGAAAACTGTTAATG | 300/300 |
Rln | AAGCACTCGCAAACAAAATTACAT | CCTAAGCGACCTTCGTCTTCTG | 900/900 |
HspA5 | CCTCAGAGTGGAGTTGAAAATGCTA | GACCCCAAGACATGTGAGCAA | 300/900 |
Ldlr | ACCTGGCTCGGTTTTCATTCT | AGAGTATCACCCCAGCCTAACCT | 900/900 |
Scd1 | GACCAGTCAAAGTGCAAGACTACCTA | AAGGTTTCCTGCAATGGTTTTC | 300/300 |
Nrp1 | CGGTAACAACAGGAATCATGTACAA | TTACCCAAATGAAACCAAGAGAAGA | 300/300 |
Mtap1B | CCGTTGCACCTTTCGTAGCT | AGCCAATGCAAGACAAAGGAA | 100/100 |
Alcam | GGACACATATCTTGCCCAATCAG | ATCCTATGGTGCTCCTAACTCTCAA | 300/900 |
HNRPDL | TTGTAAAAGACTTTGTACTCTAGATCAGAGA | TGGCAGCTATATAGACTTCCAGAGA | 300/900 |
Tnrc6a | ATGTTGGACACCGTAACCTAAGC | TATGGACATCAACACACACCGAAT | 300/300 |
VEGF | ATGCTCCCCGGGGTGTAT | CATAGGAGTCCTTCTGACCCATTC | 600/600 |