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Table 11 Comparison of QPCR and microarray data of the 15 genes selected for verification

From: Downregulation of genes with a function in axon outgrowth and synapse formation in motor neurones of the VEGFδ/δ mouse model of amyotrophic lateral sclerosis

  

Microarray result

QPCR result

Time point

Gene Symbol

FC

P value

n

FC

P value

3 months

Zfp101

+4.35

0.01

6

No change

 
 

Fos

-2.32

0.03

6

-2.59

0.0003

 

Reelin

-1.88

0.004

6

-1.68

0.03

 

HspA5

-1.44 to -1.46

0.02 to 0.01

6

-1.34

0.06

5 months

Fos

-2.17

0.01

6

-2.13

0.0007

 

Ldlr

-2.14

0.02

6

-1.42

0.006

 

Scd1

-2.07

0.02

6

-1.19

0.01

 

Nrp1

-1.58 to -2.02

0.02 to 0.009

6

No change

 
 

Mtap1B

-2.10

0.01

6

No change

 
 

Alcam

-1.60 to -1.94

0.03 to 0.02

6

-1.43

0.01

14 months

Reelin

-2.41

0.007

3

-2.1

0.008

 

HSPA5

-1.48

0.004

3

-1.27

0.2

 

Hnrpdl

-2.20

0.005

3

-1.21

0.2

 

Tnrc6a

-1.43 to -1.57

0.004 to 0.0004

3

No change

 
 

Alcam

-1.50 to -1.65

0.04 to 0.004

3

-1.59

0.003

  1. Significant results are highlighted in bold; n = no. of transgenic mice used for QPCR verification, with an equal number of gender matched littermate controls.