Table 2 Quantitative (Real-Time) RT-PCR information
From: Functional genomics of mountain pine beetle (Dendroctonus ponderosae) midguts and fat bodies
Feature | Tentative I.D. | Primer pair (Forward/Reverse) | Amplicon Length (bp) | Tm (°C) | Amplification Efficiency (%) |
|---|---|---|---|---|---|
Contig1127 | P450 (CYP6) | ACTTCCCGCTGGATACAGACAT/GGATAAGACATCGTCTGGATTGTTG | 101 | 76 | 90.91 |
Contig1103 | P450 (CYP6) | AATGACTGCTTCGGTGCTGAA/ATTCCATGTCCCTACGATTGTGT | 113 | 79 | 90.80 |
DPG001G12 | P450 (CYP4) | CCGTAATCCCACAATGTTCGA/CCAAGGCAAAGTCTAGGTCCAT | 117 | 78 | 92.41 |
Contig64 | P450 (CYP6) | GCAAGAGGAATCAACCGCTAA/CTATGCTGCCTCAGCTCGTTATT | 128 | 79 | 86.46 |
Contig608 | P450 (CYP6CR1) | GAGGAACCACATAGTTGTCATGGA/CAAAAGGGAGGCGGATGTTA | 141 | 73 | 93.3 |
Contig160 | P450 (CYP6) | AATGATTGCTTCGGCACTGAA/GGATTTGAGTAATTCCATGCTCCTA | 113 | 76 | 105.64 |
MPB029F09 | P450 (CYP6) | ACTGGTAACGGACTACGATCACTTT/TGAACGCAATACTTTCCATTCG | 115 | 75 | 89.16 |
MPB019E07 | HMGR | CCAATCACCCGTGGGAAGT/CGAAGTGGAGGTTGCTGTTCA | 82 | 81 | 95.45 |
Contig126 | GGPPS | TGAACGTGCCCAAAGAGAATT/TCGGCTAGTTTAGCTCGGATATTT | 101 | 77 | 92.12 |